LOCUS       sequence-263653-        9563 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of nucleo-cytoplasmic mTagBFP2 in P-cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0761
FEATURES             Location/Qualifiers
     source          1..9563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2526..2981)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2663..2684)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2875..2894
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3107..3129
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3121..3138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3122..3138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3148..3167
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3148..3166
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3191..3207
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3192..3208
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5329..6039
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      6063..6250
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6118..6142)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6325..7246
                     /label=bleMX6
                     /note="yeast selectable marker conferring phleomycin 
                     resistance"
     promoter        6325..6668
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6669..7043
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVSDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVDEEQD"
     terminator      7049..7246
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7303..7323)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7303..7321)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7342..7358)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7342..7358)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7355..7377)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7366..7382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7390..7396,7397..7414,7415..7420))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7435..7456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7573..7590)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7744..8332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7824..7843)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8503..9294,9295..9363))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9126..9145
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9364..9468)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9536..9554
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg gggccttacg aagttaaatt ataggcacag acatctttgg cattggaaag
      241 atttagtaac aatcataatg tagcacttct taaaaaatct aattttagaa caaaaaggct
      301 taaaaaagaa tggttccgct cttttaaaaa atagtaaaat gtacaatagc aaggttttcg
      361 taggtcgacg gacataacaa ggggaaagag aaataaagca tgtaaacgtc aataaaacat
      421 ctttgattct ggatcaatca tatgccaaat gaagaaaaat agcatttata acaacatgaa
      481 accatatttg agacctttta acgatgaaaa tttattaaat aaaagataaa aaagaaagaa
      541 accgttcaag ttcaatgaaa atttcgtcgt gaagtagaaa ccaagataat ggaaaggctt
      601 cgcaaagcca caaattttcc aatagtcatt ataactgaag attaaggaca gtatactagt
      661 aaaaatcaag tgaattgtgc ctaggatagg tagctaacgt gactgccact gaacctgtgt
      721 agcgtattag atccatcaag atcccttctg ctcatatcat tgctgatctg taaattgacc
      781 gcatcaaatt tgatctcatt tatatcgaga ttgatctata ttattttgcc aatttgcagt
      841 atataaactt atttgattaa gtttttggtc tccttcgcca cgtcttcatt ttgttagttt
      901 aaattttttt gactaactta gaaagggaag tttttaaaaa cgttgaaata ctttttcgat
      961 ttaaaaatat atatttaatt aattttctgt tccaaaatgg ttgctcctca tctttggtta
     1021 cgtgctgaaa ccaaaccttt agaagagcgc agtgctttga ctcctagaac tgccaagata
     1081 ctcgccgatg ctggatttca aattaccatt gagcgctctt cccaacgtgc tttcaaggac
     1141 aaagaatttg agcgccttgg tttccccatg gttcctgagg gctcatggcg acatgcacct
     1201 aaggacgcgt acattattgg tctcaaagag cttccggaaa atgataacag tcctttgaag
     1261 catactcata ttcaatttgc tcattgctac aaaaaccagg aaggttggcg tgaagtcttg
     1321 tcccgattcc ccgctggcaa tggtcttcta tatgatttgg aatttttgca agatgacaat
     1381 ggcagacgtg ttgccgcttt tggttaccac gctggttttg ctggatcagc tatctcatgt
     1441 ttagtttggg cacatcaatt attgcatcct aacaaacaat tccctgctat ccgtccgttc
     1501 cccaacgaaa aatctttggt tcgccatgtt gctcgtcaag tacgtttagc tttgaaaaag
     1561 aataacaacc agtatcctcg tattctggtt attggggctc ttggtcgttg tggtactgga
     1621 gcgtgcgatt tagcatcaaa gattggtatt cccttcgata acattcttcg ctgggacatt
     1681 aatgaaacta agaagggtgg tccctttacc gaaattacgg aatcagacat atttgttaac
     1741 tgcatttatc tgagcatgcc tattcccaaa ttctgtaccg tcgagtcgtt aaatgtacct
     1801 aaccgtaaat tgcgtgttgt ttgtgatgtt agctgtgaca ctaccaatcc taacaatcct
     1861 attccaattt ataatgtaaa cactacattt gatcatccta cagttgaggt taaaggtgtc
     1921 acaactcctc ctcctttaga ggtcatttct attgatcatc ttcctacttt attgccaagg
     1981 gaatcttccg aagcatttag tgaagctcta attcctagtc ttttggcttt gaaggacgtc
     2041 gacaatgctc ccgtttgggt cagagctaaa aaactttatg aaaccatggt tcaaaagctt
     2101 taaaataaat gttatttaat ttcaaagttc cgggtccttt atattgtttc accaaattat
     2161 tgaatattat tttctcaaaa attaacttta gaatacaatt tagacggcat tttcgatgct
     2221 ctggacattt tttccacgta cttgttaaat tgtagcggtg ttgcataata ctaccttttg
     2281 atttgttatt cacgttacaa tatatatttg actaatttaa atatgataat atacacattt
     2341 ctctgcaaac ctcctttata cttttcgccg gtcattgatt aataattaag aaacaggatt
     2401 tcatcctgca tttgccactt acctatatgg taaatataaa tcttgaaact ttccccaata
     2461 tttattgctt gctagggcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2521 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2581 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2641 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2701 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2761 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2821 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2881 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2941 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg
     3001 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     3061 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     3121 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc
     3181 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagaagcat
     3241 gcacgctgct cacttacaag ataacctttt gttgaaaagg aagatttatt aactatatat
     3301 gattgaagat tttttctttt caaaggatat ataagttctg caaagtttcc taaaaaaatc
     3361 tttctttttt gtttcttaca ccttttgaca tttatattgg agcctaacgt tttgccgctt
     3421 tgatatcatt tatccgttga ttttagaaaa aaaagttagg aagagttgtt accgattttt
     3481 tccttctttt tatgccaata ttcacttcat cagatttaat ttggctactt tttttttcgt
     3541 aacctactat agttatctca ttatagaatg aaatgaaact ctattattat gtagttaaaa
     3601 tcaatgataa gcactgtatt aaagtttaag agaaaatcgc gaattccttt tgatgtcctt
     3661 ttctcaaagg atacaaacac gatttataac acctttgctt cagacgtttg attgatatgc
     3721 ctattgtgat atacgaaaca tcagcatcag gttatagccg tgcaacattt tctgataaat
     3781 gatgaggaaa acagatgcta ttgtcttgct catgttggtt ctctgggttg agataatgaa
     3841 catattggta tataaaactt gcacacggat caacgagaat tgcttacttt tattgctctt
     3901 acattttatt gcttttattt tatataacat tgattgctct cttcttttct tgtactaaaa
     3961 gaaatacaaa catacaaata tggcttacat gttttacttc gcagaattat aattaattaa
     4021 atacccaagt tattcgttag catggttgag tatattgttt aataatagcg ctatttaagt
     4081 cgtaacttta taggataagt attaccagta ctaatacgtg taagaagtta cctagtgcta
     4141 ttgttaacaa aatataagct cgttaatgtg agtaacttgt tttattttct acttgaaatt
     4201 tttaacaaac aaaccagtta aaaaaaattg atctggatca atactccagc tatttggaaa
     4261 gaattgactt acgcgtaaat gtgtacgcgt taatgtttac tgatttcgtt ttcgggttta
     4321 gagaagtaaa ggatggaatt agattacagg aaagagtatt tttataaatt atatataaag
     4381 gaagatatga ctaacggcta gtgtaccaat tccataatat accgagtgca attcaaacca
     4441 aactctcgta tataggctgc gatttataaa gtaaaacttc agcgcatcag cacaattgtt
     4501 cacgagtaca aaatctaaaa agaaattttc tttgcaatgc gctcatcaat taatataaac
     4561 gaccgcgata tatgaagtca aacagtcgtt aaacaccaaa atttctttac gtatttactt
     4621 cgcagattac ccatcaaaaa gatattctat ggatttatta aagataaaga attcgctttt
     4681 gatgcctcaa attggaatac tatgctttag ataatcaaat gggctgaact ctttgaccga
     4741 ccgaaaatgg atctctatgc tgaggcttct cacggtaggt atataaagag cctactgccc
     4801 ttcttcaata tgccaaataa atacaaacaa ggttctatac tcaacagtaa tttttcttac
     4861 tcatttaatt acgaaccttg ctctgttttc gcaagttgtt ttcaagtttt caagcttcgc
     4921 gcgggatatc tggcgagcga tcaaaggtgt ttccaatagc tttaaccttt tcgtttcctg
     4981 tcctgtattt ttaaataata cattttccgt gtctattact tttattttgg aacaatattg
     5041 aagttcaaat tgtacacgac tttggcaatc ttaggaaaat cttggcatat acaattccaa
     5101 tttttgctct atccaaatat caagactacc atcatcactg aatttatact cttttgtttg
     5161 cgcatcgtct ttcggctttc tttttcacct tctttaatat tttgaaattc tatataggtc
     5221 tttgtgtacc atttaatttt tgtatcttac tttcttttat atcatattta ttagttactt
     5281 atacgttgag tttaccgtcg actctagagg atccccgggt taattaacat ggtgtctaag
     5341 ggcgaagagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     5401 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     5461 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     5521 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     5581 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     5641 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     5701 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     5761 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     5821 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     5881 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     5941 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     6001 tactgcgacc tccctagcaa actggggcac aagcttaatt aaggcgcgcc acttctaaat
     6061 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     6121 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     6181 ctttcctgta ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca
     6241 cctctaccgg cagatccgct agggataaca gggtaatata gatctgttta gcttgcctcg
     6301 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
     6361 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
     6421 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
     6481 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
     6541 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
     6601 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
     6661 aaacaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     6721 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     6781 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     6841 tgtcggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     6901 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     6961 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     7021 tcgtggacga ggagcaggac tgatcagtac tgacaataaa aagattcttg ttttcaagaa
     7081 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
     7141 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
     7201 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata
     7261 ctaacgccgc catccagttt aaacgagctc cagcttttgt tccctttagt gagggttaat
     7321 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7381 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7441 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7501 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7561 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7621 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7681 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7741 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7801 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7861 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7921 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7981 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8041 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8101 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8161 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8221 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8281 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8341 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8401 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8461 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8521 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8581 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8641 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8701 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8761 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8821 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8881 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8941 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9001 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9061 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9121 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9181 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9241 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9301 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9361 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9421 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9481 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     9541 gcgtatcacg aggccctttc gtc
//
