LOCUS       sequence-263852-         961 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal
            hexa-Histidine tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Ranaghan MJ, Durney MA, Mesleh MF, McCarren PR, Garvie CW, Daniels 
            DS, Carey KL, Skepner AP, Levine B, Perez JR
  TITLE     The Autophagy-Related Beclin-1 Protein Requires the Coiled-Coil and 
            BARA Domains To Form a Homodimer with Submicromolar Affinity.
  JOURNAL   Biochemistry. 2017 Dec 26;56(51):6639-6651. doi: 
            10.1021/acs.biochem.7b00936. Epub 2017 Dec 14.
  PUBMED    29185708
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.7b00936"; journalName: "Biochemistry"; date: 
            "2017-12-26- 26"; volume: "56"; issue: "51"; pages: "6639-6651"
COMMENT     Sequence Label: BPV00605 pET21GG2-His(6)-BECN(1-265)
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             95..112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             119..136
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             143..163
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gagccatcat catcaccatc atggatccct
      121 ggttccgcgt ggtagcgcta gcgaaaatct gtattttcag ggaatggaag gtagcaaaac
      181 cagcaataat agcaccatgc aggttagctt tgtttgtcag cgttgtagcc agccgctgaa
      241 actggatacc agctttaaaa tcctggatcg tgttaccatt caagaactga ccgcaccgct
      301 gctgaccacc gcacaggcaa aaccgggtga aacccaagaa gaagaaacca attcaggcga
      361 agaaccgttt attgaaacac cgcgtcagga tggtgttagc cgtcgtttta ttccgcctgc
      421 acgtatgatg agcaccgaaa gcgcaaatag ctttaccctg attggtgaag caagtgatgg
      481 tggcaccatg gaaaatctga gccgtcgtct gaaagttacc ggtgacctgt ttgatatcat
      541 gagcggtcag accgatgttg atcatccgct gtgtgaagaa tgtaccgata ccctgctgga
      601 tcagctggat acccaactga atgttaccga aaatgaatgc cagaactata aacgctgtct
      661 ggaaattctg gaacaaatga acgaagatga tagcgaacag ctgcagatgg aactgaaaga
      721 actggcactg gaagaagaac gtctgatcca agaactggaa gatgtggaaa aaaaccgtaa
      781 aattgtggcc gagaatctgg aaaaagttca ggcagaagcc gaacgcctgg atcaagaaga
      841 ggcacagtat cagcgtgaat atagcgaatt taaacgtcag cagctggaac tggacgatga
      901 actgaaaagc gttgaaaatc agatgcgtta tgcacagacc cagctggaca aactgaaata
      961 a
//
