LOCUS       sequence-264063-        1992 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Mammalian expression of the centrosomal protein CENPJ N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-CPAP
FEATURES             Location/Qualifiers
     source          1..1992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc accatgagga agctacaaaa ggaacgtaaa
      901 gtttttgaaa agtatactac agctgcaaga acttttccag ataaaaagga acgtgaagaa
      961 atacagactt taaaacagca aatagcagat ttacgggaag atttgaaaag aaaggagacc
     1021 aaatggtcaa gtacacacag ccgtctcaga agccagatac aaatgttagt cagagagaac
     1081 acagacctcc gggaagaaat aaaagtgatg gaaagattcc gactggatgc ctggaagaga
     1141 gcagaagcca tagagagcag cctcgaggtg gagaagaagg acaagcttgc gaacacatct
     1201 gttcgatttc aaaacagtca gatttcttca ggaacccagg tagaaaaata caagaaaaat
     1261 tatcttccaa tgcaaggcaa tccacctcga agatccaagt ctgcacctcc tcgtgattta
     1321 ggcaatttgg ataagggaca agctgcctct cccagggagc cacttgaacc actgaacttc
     1381 ccagatcctg aatataaaga ggaggaggaa gaccaagaca tacagggaga aatcagtcat
     1441 cctgatggaa aggtggaaaa ggtttataag aatgggtgcc gtgttatact gtttcccaat
     1501 ggaactcgaa aggaagtgag tgcagatggg aagaccatca ctgtcacttt ctttaatggt
     1561 gacgtgaagc aggtcatgcc agaccaaaga gtgatctact actatgcagc tgcccagacc
     1621 actcacacga catacccgga gggactggaa gtcttacatt tctcaagtgg acaaatagaa
     1681 aaacattacc cagatggaag aaaagaaatc acgtttcctg accagactgt taaaaactta
     1741 tttcctgatg gacaagaaga aagcattttc ccagatggta caattgtcag agtacaacgt
     1801 gatggcaaca aactcataga gtttaataat ggccaaagag aactacatac tgcccagttc
     1861 aagagacggg aatacccaga tggcactgtt aaaaccgtat atgcaaacgg tcatcaagaa
     1921 acgaagtaca gatccggtcg gataagagtt aaggacaagg agggtaatgt gctaatggac
     1981 acggagctgt ag
//
