LOCUS       sequence-264073-        2817 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep72 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetD-SNAP-Cep72
FEATURES             Location/Qualifiers
     source          1..2817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc accatggcgc gggctggccc tcggctggtg
      901 ctgagcgagg aggcggttcg ggcgaagagc ggcttagggc ctcaccgcga cctggctgag
      961 cttcagtcat tgtctattcc tggaacttac caagagaaga tcacccacct gggacattct
     1021 ctgatgagtt taacaggtct gaaatctttg gatctctcgc gcaactcctt ggttagtctg
     1081 gagggcattc agtacctgac tgcattggag agtctcaatc tctactacaa ctgcatctcc
     1141 tcgttggcag aagtgtttcg gctccacgcc ttaaccgagc tcgtggatgt ggacttccgg
     1201 ctgaaccccg tggtgaaggt tgagcctgac taccgccttt ttgttgtgca cctgctcccc
     1261 aagctccagc agctggacga tcgccccgtg agagcaagcg agcggaaggc ttcccgactg
     1321 cattttgcat cagaggactc actcgactcc aaagagagcg tcccagcttc tttgaaagag
     1381 ggcagaccac accaccccag agccaagtgc accgaggcct tggccaagca gagcctggtc
     1441 atggatgcgg atgacgaggc agtcctgaac ctcattgcag agtgcgagtg ggacctcggc
     1501 aggcctcccg ggagcacgag cttcagccag aaggggcgtg aggccgactc tcgtggttcc
     1561 caagaatcca gacatctgtt gagcccgcag ttggtacagt accagtgtgg ggactctggg
     1621 aagcagggcc gtgagacgag gaggagcagc tgcagagggt gctgtctgga gaagatgcct
     1681 tggagccagc tctgtggaga gcttccgcca ctgtacggag cggagccaga ggcctcccgt
     1741 gcccccaggc cacacacgta cttcacccca cacccagact ccatggatac cgaggactcg
     1801 gcctcttctc agaagttgga tttgtcagga gaaatggtgc ctggtcccct gccagccccc
     1861 ggaaagtgca ggaagcgaag aatgcctgtt ggaagattcc agacgttttc ggaccaggag
     1921 ggtttgggct gcccggagag aactcatggg tcctccgtgc ccaaggagag cctgagcaga
     1981 caggacagct cagaaagcag gaacgggagg accttgtctc agcctgaggc ctcggagact
     2041 gaggagcaga ggtctcgggg tgtgaccgac accagagagc cgtctcccgg gtcacactcg
     2101 gctctacccg ggaagaagac ggccctgcag gcggcgctcc tggagacgct cttggacctg
     2161 gtggacagga gctggggcgg ctgcaggtcc ctgcacagca acgaggcatt ccttgctcag
     2221 gcaagacaca tcttgtcatc tgttgaagaa ttcacagcag ctcaggacag ctctgcgatg
     2281 gtgggtgaag atgtcggctc cctggctctg gagagtaagt ccctgcaaag ccgccttgct
     2341 gagcagcagc agcagcacgc ccgggagatg agcgaggtga cggcggagct gcaccacaca
     2401 cacaaggagc tggatgattt gagacaacat ttagataaat ctttggaaga gaacagtagg
     2461 ttaaaatcgc ttttgttgag tatgaaaaag gaagtgaaga gtgcagacac tgcagccacg
     2521 ttaaatttgc agatcgctgg acttcaaaca agtgtgaaga ggctgtgtgg cgagattgtg
     2581 gaactgaagc agcacctgga gcactacgac aagatccagg agctcacgca gatgctgcag
     2641 gagagccaca gctccctggt cagcaccaat gaacacctgc tgcaggagct gagccaggtg
     2701 cgggcgcagc acagagccga ggtggagcag atgcactgga gctaccagga gctcaagaag
     2761 accatggccc tgtttccaca cagcagcgcc agccatggag gctgccaggc ctgctag
//
