LOCUS       sequence-264129-        3471 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep97 N-terminally 
            fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetDBl-CLIP-Cep97
FEATURES             Location/Qualifiers
     source          1..3471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgataca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttctgag
      601 tcacacttag cagccctggt aggcaacccc gcagccacgg cagcagtgaa tacggcactg
      661 gatggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggattcggat
      721 gtggggccgt accttggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttaatggcgg tggcgcgcgt ggacgcggct
      901 ttgcctcccg gagaaggatc agtggtcaat tggtcaggac agggactaca gaaattaggt
      961 ccaaatttac cctgtgaagc tgatattcac actttgattc tggataaaaa tcagattatt
     1021 aaattggaaa atctggagaa atgcaaacga ttaatacagt tatcagtagc taataatcgg
     1081 ctggttcgga tgatgggtgt ggccaagctg acgttgcttc gtgtattaaa tttgcctcat
     1141 aatagcattg gctgtgtgga agggctaaag gaactagtac atctggaatg gctgaatttg
     1201 gcaggaaata atcttaaggc catggaacag atcaatagct gcacagctct acagcatctc
     1261 gatttatcag acaataatat atcccagata ggtgatctat ctaaattggt atccctgaaa
     1321 accctgcttt tacatggaaa catcatcacc tctcttagaa tggcacctgc ttacctaccc
     1381 agaagtcttg ctatactttc tttggcagaa aatgaaatcc gagacttaaa tgagatctct
     1441 tttttggcat ccttaactga attggaacag ttgtcgatta tgaacaatcc ttgtgtgatg
     1501 gcaacaccat ccatcccagg atttgactat cggccgtaca tcgtcagctg gtgcctaaac
     1561 ctcagagtcc tagatggata tgtgatttct cagaaggaaa gtttgaaagc tgaatggctc
     1621 tatagtcaag gcaaggggag agcatatcgg cctggccagc acatccagct tgtccaatat
     1681 ctggctacag tctgccccct cacttctaca ctaggtcttc aaactgcaga ggatgccaaa
     1741 ctagagaaga ttttgagcaa acagaggttt caccagaggc agttgatgaa ccaaagccaa
     1801 aatgaagagt tgtctcctct tgttcctgtt gaaacaaggg catcccttat tcctgagcat
     1861 tcaagccctg ttcaagattg ccagatatcc caggaaagtg aacccgtcat tcaagtgaat
     1921 tcttgggttg ggataaacag taatgatgat cagttatttg cggttaagaa taattttcca
     1981 gcctctgtac acactacgag atattctcga aatgatctgc acctggaaga catacagacg
     2041 gatgaggaca agttaaactg tagtcttctc tcttcagagt ctacttttat gccagttgca
     2101 tcaggactgt ctccactatc acctacagtt gagctgaggc tgcagggcat taacttgggc
     2161 ctagaagatg atggtgttgc agatgaatct gtgaaagggc tggaaagcca ggtgttggat
     2221 aaggaagagg aacagccttt atgggctgca aatgagaatt ctgttcaaat gatgagaagt
     2281 gaaatcaata cagaggtaaa tgagaaagct ggactattac cttgtcctga gccaacaata
     2341 atcagtgcta tcttgaagga tgataaccac agtcttacat tttttcctga gtcaactgag
     2401 cagaaacaat cagacataaa gaaaccagaa aatacacaac cagaaaataa agaaaccata
     2461 tctcaagcaa cttcagagaa acttcccatg attttaaccc agagatctgt tgctttggga
     2521 caagacaaag ttgcccttca gaaattaaat gatgcagcca ccaagcttca ggcctgttgg
     2581 cggggatttt atgccaggaa ctacaaccct caagccaaag atgtgcgtta cgaaatccgg
     2641 ctacgcagaa tgcaagagca cattgtctgc ttaactgatg aaataaggag attacgaaaa
     2701 gaaagagatg aagaacgtat taaaaaattt gtacaagaag aagctttcag attcctttgg
     2761 aaccaggtaa ggtctctaca ggtttggcaa cagacagtgg accagcgtct aagttcctgg
     2821 catactgatg ttcctcctat atcaagtact cttgtgccat cgaaacatcc attatttacc
     2881 caaagccagg agtcctcttg tgatcaaaat gctgattggt ttattgcttc tgatgtagct
     2941 cctcaagaga aatcattacc agaatttcca gactctggtt ttcattcctc tctaacagaa
     3001 caagttcatt cattgcagca ttctttggat tttgagaaaa gttccacaga aggcagtgaa
     3061 agctccataa tggggaattc cattgacaca gtcagatatg gcaaagaatc agatttaggg
     3121 gatgttagtg aagaacatgg tgaatggaat aaggaaagct caaataacga gcaggacaat
     3181 agtctgcttg aacagtattt aacttcagtt caacagctgg aagatgctga tgagaggacc
     3241 aattttgata cagagacaag agatagcaaa cttcacattg cttgtttccc agtacagtta
     3301 gatacattgt ctgacggtgc ttctgtagat gagagtcatg gcatatctcc tcctttgcaa
     3361 ggtgaaatta gccagacaca agagaattct aaattaaatg cagaagttca agggcagcag
     3421 ccagaatgtg attctacatt tcagctattg catgttggtg ttactgtgta a
//
