LOCUS       sequence-264181-        3057 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep78 N-terminally 
            fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetBl-CLIP-Cep78
FEATURES             Location/Qualifiers
     source          1..3057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgataca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttctgag
      601 tcacacttag cagccctggt aggcaacccc gcagccacgg cagcagtgaa tacggcactg
      661 gatggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggattcggat
      721 gtggggccgt accttggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttgaaggaat tcggtaccat gatcgactcc
      901 gtgaagctgc gccgcgacag cgcggcggac ttcttctccc actacgagta cctgtgcgcg
      961 ctgcagaact ccgtgccgct gcccgccgtg cgcgcctgtc tccgggaggg cgtgctggat
     1021 ttcaacgccg acggcctccg cggggtggac tgggcgcctc tgctgagcac cctcaagatc
     1081 aataaagacc tgcccttggt ctccatcaag agcttcttcc agccctggct gggggacaca
     1141 ggttctgaca tgaataaatt ttgcagaagt cgtgttcctg cgataagata caaagatgtg
     1201 accttccagt tgtgtaaagc tcttaaaggc tgtttaagta tatcaagtgt gctaaagaac
     1261 ctggagctaa atggactaat tctgagagag agggatttaa ctattctagc aaagggattg
     1321 aataaatcgg cttctttggt gcacctgtct cttgcaaatt gtccaattgg agatggaggt
     1381 ttagaaatta tttgtcaagg tataaggagc tctatcactc ttaagacagt caacttcaca
     1441 ggatgtaatc tgacatggca gggagcagat cacatggcca agatcttaaa gtatcagacc
     1501 atgagaaggc atgaagaaac ctgggctgag agtcttcgct ataggagacc tgatcttgac
     1561 tgtatggctg gcttaagacg tatcacactg aattgcaaca cacttattgg tgacctaggt
     1621 gcatgtgctt ttgcagactc tctcagtgag gatttatggc tgagagctct tgacctgcaa
     1681 cagtgcggcc tcaccaatga aggagcaaag gctttgctag aggcccttga aaccaataca
     1741 actctggtcg ttctggatat aagaaaaaat ccactcattg atcattctat gatgaaagca
     1801 gttatcaaaa aagtcctcca gaatggaagg agtgccaaat cagagtacca gtggataact
     1861 tctccatcag tgaaggaacc atccaaaact gctaaacaga aaaggagaac tataattcta
     1921 ggaagtggtc acaaaggaaa agctactatt agaattgtag gattggctac aaagaaacct
     1981 gtaagtagtg gcagaaaaca ctcccttggt aaagaatatt atgcgcccgc acctcttcca
     2041 cctggtgtgt ctggtttctt gccgtggcgt actgcagaac gtgcaaaaag acacaggggt
     2101 ttcccattaa tcaaaacacg tgatatatgt aatcagttgc agcaaccagg ttttcctgtg
     2161 actgtgacag tagagagtcc ttcatcctct gaagttgaag aggttgatga ttcttcagag
     2221 agtgttcatg aagtgcctga gaaaactagt atagaacaag aagcattaca ggaaaaactg
     2281 gaggagtgcc taaagcagtt aaaggaagaa agagtgataa ggcttaaggt tgataaacga
     2341 gtcagtgagc tggaacatga aaatgcccag ttaagaaata taaatttctc tttgtctgaa
     2401 gcccttcatg cacagtcatt gacaaatatg atcctggatg atgaaggtgt tttgggcagc
     2461 attgagaatt cttttcagaa gtttcatgct ttcttggatc tccttaaaga tgctgggctt
     2521 gggcagcttg ccacaatggc tgggatagat cagtcagatt ttcaattact aggtcatccc
     2581 cagatgactt ctactgttag taatccacct aaagaagaaa agaaggcgct tgaagatgaa
     2641 aaaccagaac cgaagcagaa tgccctaggg caaatgcaaa atatccaggt ttctatttgt
     2701 atgcagtcag cttacaatga aggaacacta atgaagtttc agaaaattac aggtgatgct
     2761 agaattcctt tgcctctcga ctcctttcct gtcccagttt ctactccaga gggcttagga
     2821 acttccagca acaacctagg agtcccagct actgagcagc ggcaggagtc ttttgaagga
     2881 ttcattgcta gaatgtgttc tccttcacca gatgcgactt ctggaactgg aagtcaaaga
     2941 aaagaagagg agttgtccag aaatagcaga tcttcttcag agaaaaagac caaaacaggt
     3001 gaatatacca aaaaacactc tgataagcaa caccctggaa aggacctgca ttcctag
//
