LOCUS       sequence-264351-        9705 bp    DNA     circular SYN 14-APR-2025
DEFINITION  a retroviral vector to express lysyl oxidaseL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9705)
  TITLE     CLEZARDIN Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQXCINeo-LOXL2-BC
FEATURES             Location/Qualifiers
     source          1..9705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..585
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             586..761
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    824..1023
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1224..1640
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1570..1592
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1608..1624
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1677..1980
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1981..2184
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2181..2205
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4797..5259
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4853..4870)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5080..5099
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5273..6067
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5327..5346)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5937..5956
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6484..6909
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7012..7034)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7170..7190)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7175..7504
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7355..7490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7526..7545
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7626..7643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7797..8385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7877..7896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8556..9347,9348..9416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9179..9198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9417..9521)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9589..9607
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcaata aaagagccca
      601 caacccctca ctcggcgcgc cagtcttccg atagactgcg tcgcccgggt acccgtattc
      661 ccaataaagc ctcttgctgt ttgcatccga atcgtggtct cgctgttcct tgggagggtc
      721 tcctctgagt gattgactac ccacgacggg ggtctttcat ttgggggctc gtccgggatt
      781 tggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt
      841 atctgtgtct gtccgattgt ctagtgtcta tgtttgatgt tatgcgcctg cgtctgtact
      901 agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac
      961 ccggccgcaa ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct
     1021 gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg
     1081 agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc
     1141 gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     1201 ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag
     1261 gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac
     1321 gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg
     1381 gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc
     1441 atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc
     1501 ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc
     1561 cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
     1621 tcactccttc tctaggcgcc ggaattgaag atctggggga tcgatcctct agagtccgtt
     1681 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     1741 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     1801 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     1861 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     1921 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     1981 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2041 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2101 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2161 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2221 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa
     2281 cggtgcattg gaacggacct gcaggaattg atccgcggcc gcaccggtag gcctcgtacg
     2341 cttaattaac ggatccaaga cagggatgga gaggcctctg tgctcccacc tctgcagctg
     2401 cctggctatg ctggccctcc tgtcccccct gagcctggca cagtatgaca gctggcccca
     2461 ttaccccgag tacttccagc aaccggctcc tgagtatcac cagccccagg cccccgccaa
     2521 cgtggccaag attcagctgc gcctggctgg gcagaagagg aagcacagcg agggccgggt
     2581 ggaggtgtac tatgatggcc agtggggcac cgtgtgcgat gacgacttct ccatccacgc
     2641 tgcccacgtc gtctgccggg agctgggcta cgtggaggcc aagtcctgga ctgccagctc
     2701 ctcctacggc aagggagaag ggcccatctg gttagacaat ctccactgta ctggcaacga
     2761 ggcgaccctt gcagcatgca cctccaatgg ctggggcgtc actgactgca agcacacgga
     2821 ggatgtcggt gtggtgtgca gcgacaaaag gattcctggg ttcaaatttg acaattcgtt
     2881 gatcaaccag atagagaacc tgaatatcca ggtggaggac attcggattc gagccatcct
     2941 ctcaacctac cgcaagcgca ccccagtgat ggagggctac gtggaggtga aggagggcaa
     3001 gacctggaag cagatctgtg acaagcactg gacggccaag aattcccgcg tggtctgcgg
     3061 catgtttggc ttccctgggg agaggacata caataccaaa gtgtacaaaa tgtttgcctc
     3121 acggaggaag cagcgctact ggccattctc catggactgc accggcacag aggcccacat
     3181 ctccagctgc aagctgggcc cccaggtgtc actggacccc atgaagaatg tcacctgcga
     3241 gaatgggcta ccggccgtgg tgagttgtgt gcctgggcag gtcttcagcc ctgacggacc
     3301 ctcgagattc cggaaagcgt acaagccaga gcaacccctg gtgcgactga gaggcggtgc
     3361 ctacatcggg gagggccgcg tggaggtgct caaaaatgga gagtggggga ccgtctgcga
     3421 cgacaagtgg gacctggtgt cggccagtgt ggtctgcaga gagctgggct ttgggagtgc
     3481 caaagaggca gtcactggct cccgactggg gcaagggatc ggacccatcc acctcaacga
     3541 gatccagtgc acaggcaatg agaagtccat tatagactgc aagttcaatg ccgagtctca
     3601 gggctgcaac cacgaggagg atgctggtgt gagatgcaac acccctgcca tgggcttgca
     3661 gaagaagctg cgcctgaacg gcggccgcaa tccctacgag ggccgagtgg aggtgctggt
     3721 ggagagaaac gggtcccttg tgtgggggat ggtgtgtggc caaaactggg gcatcgtgga
     3781 ggccatggtg gtctgccgcc agctgggcct gggattcgcc agcaacgcct tccaggagac
     3841 ctggtattgg cacggagatg tcaacagcaa caaagtggtc atgagtggag tgaagtgctc
     3901 gggaacggag ctgtccctgg cgcactgccg ccacgacggg gaggacgtgg cctgccccca
     3961 gggcggagtg cagtacgggg ccggagttgc ctgctcagaa accgcccctg acctggtcct
     4021 caatgcggag atggtgcagc agaccaccta cctggaggac cggcccatgt tcctgctgca
     4081 gtgtgccatg gaggagaact gcctctcggc ctcagccgcg cagaccgacc ccaccacggg
     4141 ctaccgccgg ctcctgcgct tctcctccca gatccacaac aatggccagt ccgacttccg
     4201 gcccaagaac ggccgccacg cgtggatctg gcacgactgt cacaggcact accacagcat
     4261 ggaggtgttc acccactatg acctgctgaa cctcaatggc accaaggtgg cagagggcca
     4321 caaggccagc ttctgcttgg aggacacaga atgtgaagga gacatccaga agaattacga
     4381 gtgtgccaac ttcggcgatc agggcatcac catgggctgc tgggacatgt accgccatga
     4441 catcgactgc cagtgggttg acatcactga cgtgccccct ggagactacc tgttccaggt
     4501 tgttattaac cccaacttcg aggttgcaga atccgattac tccaacaaca tcatgaaatg
     4561 caggagccgc tatgacggcc accgcatctg gatgtacaac tgccacatag gtggttcctt
     4621 cagcgaagag acggaaaaaa agtttgagca cttcagcggg ctcttaaaca accagctgtc
     4681 cccgcagtaa agaagccccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     4741 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     4801 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     4861 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     4921 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     4981 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     5041 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     5101 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     5161 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     5221 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa ccatggctga
     5281 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5341 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5401 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5461 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5521 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5581 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5641 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5701 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5761 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5821 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5881 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5941 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6001 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6061 cttctgagcg ggatcggcta gagtcgaccc ggacgggacg ctcctgcgcc tgatacagaa
     6121 cgaattgctt gcaggcatct catgagtgtg tcttcccgtt ttccgcctga ggtcactgcg
     6181 tggatggagc gctggcgcct gctgcgcgac ggcgagctgc tcaccaccca ctcgagggct
     6241 gcagcgctgc agaggccgag tgcagaactg ctccaaaggg acctcaaggc tttccgaggg
     6301 acactaggct gactccatcg agccagtgta gagataagct tatcgattag tccaatttgt
     6361 taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa
     6421 gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc agaaaaaggg
     6481 gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa
     6541 ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg
     6601 aacagggtcg accctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     6661 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     6721 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
     6781 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     6841 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     6901 tctttcattt gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac
     6961 caccgggagg taagctggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     7021 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     7081 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     7141 tagcgatagc ggagtgtaga tccggctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7201 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7261 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7321 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7381 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7441 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7501 caaaaagctt actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     7561 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     7621 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7681 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7741 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7801 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7861 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7921 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7981 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8041 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8101 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8161 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8221 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8281 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8341 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8401 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8461 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8521 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8581 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8641 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8701 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8761 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8821 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     8881 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8941 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9001 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9061 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9121 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     9181 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9241 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9301 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9361 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9421 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9481 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9541 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9601 acgaggccct ttcgtcttca agaattagct tggccattgc atacgttgta tccatatcat
     9661 aatatgtaca tttatattgg ctcatgtcca acattaccgc catgt
//
