LOCUS       sequence-264481-        3225 bp    DNA     circular SYN 15-APR-2025
DEFINITION  pZE1-katGp-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: TJF64
FEATURES             Location/Qualifiers
     source          1..3225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             336..347
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             354..1064
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(499..517)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     767..786
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1100..1186
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1351..1939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1431..1450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2027..2121)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2151..2942,2943..3011))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2774..2793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3012..3116)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3184..3202
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tggcttttat gaaaatcaca cagtgatcac aaattttaaa cagagcacaa aatgctgcct
       61 cgaaatgagg gcgggaaaat aaggttatca gccttgtttt ctccctcatt acttgaagga
      121 tatgaagcta aaaccctttt ttataaagca tttgtccgaa ttcggacata atcaaaaaag
      181 cttaattaag atcaatttga tctacatctc tttaaccaac aatatgtaag atctcaacta
      241 tcgcatccgt ggattaattc aattataact tctctctaac gctgtgtatc gtaacggtaa
      301 cactgtagag gggagcacat tgatgcgaat tcattaaaga ggagaaaggt accatggtga
      361 gcaagggcga agaagataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      421 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      481 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      541 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      601 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      661 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      721 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      781 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      841 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      901 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
      961 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     1021 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaacccggg ggatcccatg
     1081 gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1141 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac
     1201 ctagggcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1261 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     1801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1981 gaacgaaaac tcacgttaag ggattttggt catgactagt gcttggattc tcaccaataa
     2041 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     2101 ctggatctat caacaggagt ccaagcgagc tcgtaaactt ggtctgacag ttaccaatgc
     2161 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2221 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2281 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2341 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2401 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2461 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2521 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2581 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2641 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2701 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2761 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2821 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2881 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2941 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3001 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3061 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3121 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3181 aaaaataggc gtatcacgag gccctttcgt cttcacctcg aggtg
//
