LOCUS       sequence-264842-        8150 bp    DNA     circular SYN 14-APR-2025
DEFINITION  W2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the 
            CAMERA2m system for cellular recording in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Tang W, Liu DR
  TITLE     Rewritable multi-event analog recording in bacterial and mammalian 
            cells.
  JOURNAL   Science. 2018 Feb 15. pii: science.aap8992. doi: 
            10.1126/science.aap8992.
  PUBMED    29449507
REFERENCE   2  (bases 1 to 8150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992."
COMMENT     Sequence Label: pWT065a
FEATURES             Location/Qualifiers
     source          1..8150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        71..102
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             164..850
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(899..4297,4298..4300,4301..4897,4898,4899..4901,
                     4902..4903,4904..4906,4907..4999)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(899..922,923..925,926..4999)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5012..5260
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5273..5293
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5302..5319
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5342..5359)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5348..5572
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5643..5659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5643..5659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5656..5678)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5667..5683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5691..5697,5698..5715,5716..5721))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5736..5757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5874..5891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6045..6633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6125..6144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6804..7595,7596..7664))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7427..7446
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7665..7769)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7848..7867)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggtaagatc aaagggggta agatcaaagg ggcgcgcccg cgtgctagcc cgggctcgag
       61 atctagactc tagagggtat ataatggaag ctcgaattcc agcttggcat tccggtactg
      121 ttggtaaaaa gcttggcatt ccggtactgt tggtaaagcc accatgagct cagagactgg
      181 cccagtggct gtggacccca cattgagacg gcggatcgag ccccatgagt ttgaggtatt
      241 cttcgatccg agagagctcc gcaaggagac ctgcctgctt tacgaaatta attggggggg
      301 ccggcactcc atttggcgac atacatcaca gaacactaac aagcacgtcg aagtcaactt
      361 catcgagaag ttcacgacag aaagatattt ctgtccgaac acaaggtgca gcattacctg
      421 gtttctcagc tggagcccat gcggcgaatg tagtagggcc atcactgaat tcctgtcaag
      481 gtatccccac gtcactctgt ttatttacat cgcaaggctg taccaccacg ctgacccccg
      541 caatcgacaa ggcctgcggg atttgatctc ttcaggtgtg actatccaaa ttatgactga
      601 gcaggagtca ggatactgct ggagaaactt tgtgaattat agcccgagta atgaagccca
      661 ctggcctagg tatccccatc tgtgggtacg actgtacgtt cttgaactgt actgcatcat
      721 actgggcctg cctccttgtc tcaacattct gagaaggaag cagccacagc tgacattctt
      781 taccatcgct cttcagtctt gtcattacca gcgactgccc ccacacattc tctgggccac
      841 cgggttgaaa agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga
      901 taaaaagtat tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac
      961 cgatgaatac aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc
     1021 gattaaaaag aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac
     1081 tcgcctgaaa cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt
     1141 acaagaaatt tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga
     1201 agagtccttc cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat
     1261 agtagatgag gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct
     1321 agttgactca actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat
     1381 aaagttccgt gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga
     1441 caaactgttc atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa
     1501 tgcaagtggc gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct
     1561 agaaaacctg atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat
     1621 agcgctctca ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc
     1681 caaattgcag cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat
     1741 tggagatcag tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct
     1801 atctgacata ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat
     1861 caaaaggtac gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca
     1921 actgcctgag aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta
     1981 tattgacggc ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa
     2041 gatggatggg acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca
     2101 gcggactttc gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat
     2161 acttagaagg caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa
     2221 aatcctaacc tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt
     2281 cgcatggatg acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt
     2341 cgataaaggt gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt
     2401 accgaacgaa aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa
     2461 tgaactcacg aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg
     2521 agaacagaag aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa
     2581 gcaattgaaa gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg
     2641 ggtagaagat cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa
     2701 agataaggac ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac
     2761 tcttaccctc tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct
     2821 gttcgacgat aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt
     2881 gtcgcggaaa cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt
     2941 tctaaagagc gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt
     3001 aaccttcaaa gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga
     3061 acatattgcg aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa
     3121 agtagtggat gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga
     3181 gatggcacgc gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa
     3241 gagaatagaa gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga
     3301 aaatacccaa ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat
     3361 gtatgttgat caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt
     3421 accccaatcc tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa
     3481 gaaccgaggg aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta
     3541 ttggcggcag ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa
     3601 agctgagagg ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt
     3661 ggaaacccgc caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa
     3721 atacgacgag aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt
     3781 ggtgtcggac ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca
     3841 ccatgcgcac gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc
     3901 gaagctagaa agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat
     3961 cgcgaaaagc gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat
     4021 tatgaatttc tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt
     4081 aattgaaacc aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac
     4141 ggtgagaaaa gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac
     4201 cggagggttt tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg
     4261 taaaaaggac tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc
     4321 tgtcctagta gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga
     4381 attattgggg ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct
     4441 tgaggcgaaa ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag
     4501 tctgtttgag ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa
     4561 ggggaacgaa ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta
     4621 cgagaagttg aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca
     4681 caaacattat ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct
     4741 agctgatgcc aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat
     4801 acgtgagcag gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc
     4861 cgcattcaag tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt
     4921 gctagacgcg acactgattc accaatccat cacgggatta tatgaaactc ggatagattt
     4981 gtcacagctt gggggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga
     5041 gaccggtaag caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga
     5101 agtcattggg aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac
     5161 cgacgagaat gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt
     5221 catacaggat agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa
     5281 gaagaggaaa gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     5341 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5401 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5461 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5521 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5581 gcggaaagaa ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca
     5641 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5701 gccggaagca taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt
     5761 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5821 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5881 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5941 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6001 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6061 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6121 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6181 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6241 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6301 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6361 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6421 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6481 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6541 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6601 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6661 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6721 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6781 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6841 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6901 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6961 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7021 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7081 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7141 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7201 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7261 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7321 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7381 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7441 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7501 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7561 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7621 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7681 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7741 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     7801 gacggatcgg gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct
     7861 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     7921 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     7981 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     8041 cattgaaggc cctatcgata ggtaccgagc tcttacgcga gatcaaaggg ggtaagatca
     8101 aagggggtaa gatcaaaggg gcgcgagatc aaagggggta agatcaaagg
//
