LOCUS       sequence-264914-        3277 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Golden Gate Level 0 S module for N-terminal fluorescent protein 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Luginbuehl LH, El-Sharnouby S, Wang N, Hibberd JM
  TITLE     Fluorescent reporters for functional analysis in rice leaves.
  JOURNAL   Plant Direct. 2020 Feb 11;4(2):e00188. doi: 10.1002/pld3.188. 
            eCollection 2020 Feb.
  PUBMED    32072132
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-02-11"; volume: "4(2):e00188. doi"; pages: " 
            10.1002/pld3.188. eCollection 2020 Feb"
COMMENT     Sequence Label: pL0M-S-mTurquoise2-EC18152
FEATURES             Location/Qualifiers
     source          1..3277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1267..1284)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1377..1965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1457..1476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(3150..3254)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccatgag ctccactctg tggtctcaaa tggcgtccaa aggggaagag
      421 ctgttcacgg gcgtcgtgcc aatactcgtg gagcttgatg gggatgtcaa tggacataag
      481 ttctccgtgt ctggggaagg agagggagat gcgacctatg gtaagctgac ccttaaattt
      541 atctgcacta ccggcaaact gccggtcccc tggcccacgc tggttacaac actgtcatgg
      601 ggggtgcagt gctttgcccg gtatcccgat cacatgaaac agcacgattt cttcaagagt
      661 gctatgcctg aaggctacgt ccaagaaaga accatatttt tcaaggatga tgggaactat
      721 aaaacacgcg ccgaagtcaa gttcgagggc gacaccctcg ttaatcgcat agaactcaag
      781 ggaatcgact tcaaggagga cgggaacatt ttgggtcata agttggagta caactacttt
      841 agcgacaacg tttatatcac tgccgacaag caaaagaacg gtatcaaagc gaactttaag
      901 atccggcaca atatcgagga cggtggtgtt caactggcgg accattatca acagaacaca
      961 cctatcggcg acggacctgt cttgttgccg gataaccatt acttgtcaac tcaatcgaag
     1021 cttagtaagg atccgaatga aaaaagagat cacatggttc tgctggagtt tgtgaccgcg
     1081 gcagggataa ctttgggtat ggacgagctg tataaaggag gttgagacca cgaagtgggt
     1141 acctcttaat taactggcct catgggcctt cacttcactg cccgctttcc agtcgggaaa
     1201 cctgtcgtgc cagctgcatt aacatggtca tagctgtttc cttgcgtatt gggcgctctc
     1261 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa
     1321 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1381 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1441 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1501 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1561 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1621 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1681 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1741 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1801 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1861 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1921 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1981 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2041 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2101 atctaaagta tatatgagta aacttggtct gacagttatt atttacccac cactttggta
     2161 atttcgcctt tcacgtaatg cacaaattct tccagctgat ccgcacggct ggccagacga
     2221 tcttcttcct ggcccagata cgcctgacgc gcttccagaa tcaccggctg atactgcgcc
     2281 ggcagacgtt ccatggccca atccgccgcc acatctttcg gcgcaatttt gccggtcacc
     2341 gcgctatacc aaatacggct cagggtcagc accacgttgc gttcatcacc cgcccaatcc
     2401 ggcgggctgt tccacagggt cagggtttcg ttcagcgctt caaacaggtc ctgttccggc
     2461 accggatcaa acagttcttc cgctgccgga ccaaccagcg ccacgctatg ttcacgcgct
     2521 ttggtcagca gaatggccag atcgatatca atggtcgccg gttcaaaaat gccggccaga
     2581 atatcgttac gctgccattc gccaaactgc agctcacgtt tcgccggata acgccacgga
     2641 ataatgtcat catgcaccac gatggtcact tccaccgcac gcagaatttc gctttcaccc
     2701 gggctcgcgc tggtttccag cagatcgtta atcagcgcac gacgggtggt ttcatccaga
     2761 cgcacggtca cggtcaccag cagatcaata tcgctatgcg gtttcaggcc gccatccacc
     2821 gcgctgccat acagatgcac ggccagcagg gtcggttcca gatgacgttc aatcacgccc
     2881 accacttcag acagctgggt gctcacttcc gcaatcaccg cttcacgcat aatgttcagt
     2941 ttggttttcg gacggctgcc ctgctgggtc acatcgttgc tgctccacag catcaggcaa
     3001 cggcccaccg cataacgcgc ctgctgtttg ctcgcacgcg gcatgctctg cacgccaaaa
     3061 aagcaatcat agcacgccat aaacaccgcc accgcaccat taccgccgct acgttcggtc
     3121 agggtacggc tccaattacg gctacgcata ctcttccttt ttcaatatta ttgaagcatt
     3181 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3241 ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
