LOCUS       sequence-265190-        8655 bp    DNA     circular SYN 14-APR-2025
DEFINITION  a retroviral vector to express lysyl oxidase (derived from 
            PCR4-TOPO-LOX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8655)
  TITLE     CLEZARDIN Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQXCINeo-LOX
FEATURES             Location/Qualifiers
     source          1..8655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..585
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             586..761
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    824..1023
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1224..1640
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1570..1592
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1608..1624
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1677..1980
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1981..2184
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2181..2205
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3747..4209
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3803..3820)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4030..4049
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4223..5017
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4277..4296)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4887..4906
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5434..5859
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(5962..5984)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6120..6140)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6125..6454
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6305..6440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6476..6495
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6576..6593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6747..7335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6827..6846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7506..8297,8298..8366))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8129..8148
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8367..8471)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8539..8557
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcaata aaagagccca
      601 caacccctca ctcggcgcgc cagtcttccg atagactgcg tcgcccgggt acccgtattc
      661 ccaataaagc ctcttgctgt ttgcatccga atcgtggtct cgctgttcct tgggagggtc
      721 tcctctgagt gattgactac ccacgacggg ggtctttcat ttgggggctc gtccgggatt
      781 tggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt
      841 atctgtgtct gtccgattgt ctagtgtcta tgtttgatgt tatgcgcctg cgtctgtact
      901 agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac
      961 ccggccgcaa ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct
     1021 gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg
     1081 agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc
     1141 gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     1201 ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag
     1261 gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac
     1321 gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg
     1381 gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc
     1441 atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc
     1501 ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc
     1561 cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
     1621 tcactccttc tctaggcgcc ggaattgaag atctggggga tcgatcctct agagtccgtt
     1681 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     1741 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     1801 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     1861 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     1921 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     1981 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2041 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2101 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2161 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2221 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa
     2281 cggtgcattg gaacggacct gcaggaattg atccgcggcc gcaccggtag gcctcgtacg
     2341 cttaattaac ggatccggtc aatctggcaa aaggagtgat gcgcttcgcc tggaccgtgc
     2401 tcctgctcgg gcctttgcag ctctgcgcgc tagtgcactg cgcccctccc gccgccggcc
     2461 aacagcagcc cccgcgcgag ccgccggcgg ctccgggcgc ctggcgccag cagatccaat
     2521 gggagaacaa cgggcaggtg ttcagcttgc tgagcctggg ctcacagtac cagcctcagc
     2581 gccgccggga cccgggcgcc gccgtccctg gtgcagccaa cgcctccgcc cagcagcccc
     2641 gcactccgat cctgctgatc cgcgacaacc gcaccgccgc ggcgcgaacg cggacggccg
     2701 gctcatctgg agtcaccgct ggccgcccca ggcccaccgc ccgtcactgg ttccaagctg
     2761 gctactcgac atctagagcc cgcgaagctg gcgcctcgcg cgcggagaac cagacagcgc
     2821 cgggagaagt tcctgcgctc agtaacctgc ggccgcccag ccgcgtggac ggcatggtgg
     2881 gcgacgaccc ttacaacccc tacaagtact ctgacgacaa cccttattac aactactacg
     2941 atacttatga aaggcccaga cctgggggca ggtaccggcc cggatacggc actggctact
     3001 tccagtacgg tctcccagac ctggtggccg acccctacta catccaggcg tccacgtacg
     3061 tgcagaagat gtccatgtac aacctgagat gcgcggcgga ggaaaactgt ctggccagta
     3121 cagcatacag ggcagatgtc agagattatg atcacagggt gctgctcaga tttccccaaa
     3181 gagtgaaaaa ccaagggaca tcagatttct tacccagccg accaagatat tcctgggaat
     3241 ggcacagttg tcatcaacat taccacagta tggatgagtt tagccactat gacctgcttg
     3301 atgccaacac ccagaggaga gtggctgaag gccacaaagc aagtttctgt cttgaagaca
     3361 catcctgtga ctatggctac cacaggcgat ttgcatgtac tgcacacaca cagggattga
     3421 gtcctggctg ttatgatacc tatggtgcag acatagactg ccagtggatt gatattacag
     3481 atgtaaaacc tggaaactat atcctaaagg tcagtgtaaa ccccagctac ctggttcctg
     3541 aatctgacta taccaacaat gttgtgcgct gtgacattcg ctacacagga catcatgcgt
     3601 atgcctcagg ctgcacaatt tcaccgtatt agaaggcaaa gcaaaactcc caatggataa
     3661 atcagtggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3721 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3781 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3841 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3901 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3961 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     4021 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     4081 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     4141 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4201 cgatgataag cttgccacaa ccatggctga acaagatgga ttgcacgcag gttctccggc
     4261 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4321 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4381 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     4441 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4501 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4561 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4621 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4681 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4741 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4801 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4861 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4921 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4981 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggatcggcta gagtcgaccc
     5041 ggacgggacg ctcctgcgcc tgatacagaa cgaattgctt gcaggcatct catgagtgtg
     5101 tcttcccgtt ttccgcctga ggtcactgcg tggatggagc gctggcgcct gctgcgcgac
     5161 ggcgagctgc tcaccaccca ctcgagggct gcagcgctgc agaggccgag tgcagaactg
     5221 ctccaaaggg acctcaaggc tttccgaggg acactaggct gactccatcg agccagtgta
     5281 gagataagct tatcgattag tccaatttgt taaagacagg atatcagtgg tccaggctct
     5341 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat agataaaata
     5401 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     5461 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     5521 gaagttcaga tcaaggtcag gaacagatgg aacagggtcg accctagaga accatcagat
     5581 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     5641 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     5701 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     5761 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     5821 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
     5881 gagacccctg cccagggacc accgacccac caccgggagg taagctggct gcctcgcgcg
     5941 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6001 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6061 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtaga tccggctgtg
     6121 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6181 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     6241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     6301 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6361 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6421 aggaggcttt tttggaggcc taggcttttg caaaaagctt actggcttaa ctatgcggca
     6481 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     6541 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6601 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6661 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7201 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7501 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7561 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7621 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7681 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7741 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7801 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7861 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7921 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7981 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8041 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8101 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     8161 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8221 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8281 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8341 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8401 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8461 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     8521 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattagct
     8581 tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca
     8641 acattaccgc catgt
//
