LOCUS       sequence-265223-       10846 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses HA tagged UBE3A in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, 
            Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, 
            Brodsky JL, Sobol RW
  TITLE     UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 
            Ubiquitin Ligase.
  JOURNAL   J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824.
  PUBMED    28003368
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824."
COMMENT     Sequence Label: pLVX-HA-UBE3A-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2824..2848
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2872..2898
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5540..5564)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5596..6170,6171..6173,6174..6182)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5763..5780)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5990..6009
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6202..6801
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6202..6221)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6698..6718
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6815..7403
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6868..6888)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7286..7297)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7610..8243
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8371..8387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8371..8387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8384..8406)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8395..8411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8419..8425,8426..8443,8444..8449))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8464..8485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8602..8619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8773..9361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8853..8872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9532..10323,10324..10392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10155..10174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10393..10497)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10545..10679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10582..10601)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10636..10655
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactaga
     2821 tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat gtacccatac
     2881 gatgttccag attacgctga gaagctgcac cagtgttatt ggaaatcagg agaacctcag
     2941 tctgacgaca ttgaagctag ccgaatgaag cgagcagctg caaagcatct aatagaacgc
     3001 tactaccacc agttaactga gggctgtgga aatgaagcct gcacgaatga gttttgtgct
     3061 tcctgtccaa cttttcttcg tatggataat aatgcagcag ctattaaagc cctcgagctt
     3121 tataagatta atgcaaaact ctgtgatcct catccctcca agaaaggagc aagctcagct
     3181 taccttgaga actcgaaagg tgcccccaac aactcctgct ctgagataaa aatgaacaag
     3241 aaaggcgcta gaattgattt taaagatgtg acttacttaa cagaagagaa ggtatatgaa
     3301 attcttgaat tatgtagaga aagagaggat tattcccctt taatccgtgt tattggaaga
     3361 gttttttcta gtgctgaggc attggtacag agcttccgga aagttaaaca acacaccaag
     3421 gaagaactga aatctcttca agcaaaagat gaagacaaag atgaagatga aaaggaaaaa
     3481 gctgcatgtt ctgctgctgc tatggaagaa gactcagaag catcttcctc aaggataggt
     3541 gatagctcac agggagacaa caatttgcaa aaattaggcc ctgatgatgt gtctgtggat
     3601 attgatgcca ttagaagggt ctacaccaga ttgctctcta atgaaaaaat tgaaactgcc
     3661 tttctcaatg cacttgtata tttgtcacct aacgtggaat gtgacttgac gtatcacaat
     3721 gtatactctc gagatcctaa ttatctgaat ttgttcatta tcgtaatgga gaatagaaat
     3781 ctccacagtc ctgaatatct ggaaatggct ttgccattat tttgcaaagc gatgagcaag
     3841 ctaccccttg cagcccaagg aaaactgatc agactgtggt ctaaatacaa tgcagaccag
     3901 attcggagaa tgatggagac atttcagcaa cttattactt ataaagtcat aagcaatgaa
     3961 tttaacagtc gaaatctagt gaatgatgat gatgccattg ttgctgcttc gaagtgcttg
     4021 aaaatggttt actatgcaaa tgtagtggga ggggaagtgg acacaaatca caatgaagaa
     4081 gatgatgaag agcccatccc tgagtccagc gagctgacac ttcaggaact tttgggagaa
     4141 gaaagaagaa acaagaaagg tcctcgagtg gaccccctgg aaactgaact tggtgttaaa
     4201 accctggatt gtcgaaaacc acttatccct tttgaagagt ttattaatga accactgaat
     4261 gaggttctag aaatggataa agattatact tttttcaaag tagaaacaga gaacaaattc
     4321 tcttttatga catgtccctt tatattgaat gctgtcacaa agaatttggg attatattat
     4381 gacaatagaa ttcgcatgta cagtgaacga agaatcactg ttctctacag cttagttcaa
     4441 ggacagcagt tgaatccata tttgagactc aaagttagac gtgaccatat catagatgat
     4501 gcacttgtcc ggctagagat gatcgctatg gaaaatcctg cagacttgaa gaagcagttg
     4561 tatgtggaat ttgaaggaga acaaggagtt gatgagggag gtgtttccaa agaatttttt
     4621 cagctggttg tggaggaaat cttcaatcca gatattggta tgttcacata cgatgaatct
     4681 acaaaattgt tttggtttaa tccatcttct tttgaaactg agggtcagtt tactctgatt
     4741 ggcatagtac tgggtctggc tatttacaat aactgtatac tggatgtaca ttttcccatg
     4801 gttgtctaca ggaagctaat ggggaaaaaa ggaacttttc gtgacttggg agactctcac
     4861 ccagttctat atcagagttt aaaagattta ttggagtatg aagggaatgt ggaagatgac
     4921 atgatgatca ctttccagat atcacagaca gatctttttg gtaacccaat gatgtatgat
     4981 ctaaaggaaa atggtgataa aattccaatt acaaatgaaa acaggaagga atttgtcaat
     5041 ctttattctg actacattct caataaatca gtagaaaaac agttcaaggc ttttcggaga
     5101 ggttttcata tggtgaccaa tgaatctccc ttaaagtact tattcagacc agaagaaatt
     5161 gaattgctta tatgtggaag ccggaatcta gatttccaag cactagaaga aactacagaa
     5221 tatgacggtg gctataccag ggactctgtt ctgattaggg agttctggga aatcgttcat
     5281 tcatttacag atgaacagaa aagactcttc ttgcagttta caacgggcac agacagagca
     5341 cctgtgggag gactaggaaa attaaagatg attatagcca aaaatggccc agacacagaa
     5401 aggttaccta catctcatac ttgctttaat gtgcttttac ttccggaata ctcaagcaaa
     5461 gaaaaactta aagagagatt gttgaaggcc atcacgtatg ccaaaggatt tggcatgctg
     5521 taaaagggtg ggcgcgccga cccagctttc ttgtacaaag tggttgatct agttctagag
     5581 cggccgcgga tcccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     5641 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5701 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5761 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5821 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5881 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5941 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     6001 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     6061 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     6121 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa
     6181 cccacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc
     6241 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6301 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6361 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6421 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6481 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6541 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6601 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6661 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6721 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6781 acccgcaagc ccggtgccta gacgcgtctg gaacaatcaa cctctggatt acaaaatttg
     6841 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6901 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6961 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7021 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7081 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7141 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7201 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     7261 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7321 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7381 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     7441 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7501 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     7561 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     7621 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7681 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     7741 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     7801 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     7861 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     7921 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     7981 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     8041 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8101 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8161 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8221 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8281 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc
     8341 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8401 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8461 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8521 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8581 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8641 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8701 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9181 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9841 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10141 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10501 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag
    10561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    10681 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    10741 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10801 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
