LOCUS       sequence-265267-        6919 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses Cherry-ICP0 fusion protein with a RING finger deletion 
            (FXE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Newhart A, Rafalska-Metcalf IU, Yang T, Negorev DG, Janicki SM
  TITLE     Single-cell analysis of Daxx and ATRX-dependent transcriptional 
            repression.
  JOURNAL   J Cell Sci. 2012 Nov 15;125(Pt 22):5489-501. doi: 
            10.1242/jcs.110148. Epub 2012 Sep 12.
  PUBMED    22976303
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-11-15"; volume: "125(Pt 22):5489-501. doi"; 
            pages: " 10.1242/jcs.110148. Epub 2012 Sep 12"
COMMENT     Sequence Label: Cherry-ICP0FxE-C3
FEATURES             Location/Qualifiers
     source          1..6919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1260
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(698..716)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     966..985
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3647..3768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3684..3703)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3738..3757
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3775..4230)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3912..3933)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4124..4143
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4257..4361
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4363..4720
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4363..4554
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4386..4406)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4571..4706
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4755..5549
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4755..5549
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4809..4828)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5419..5438
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5737..5756)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5781..5828
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6157..6745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6646..6665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1261 tactcagatc tcgacatgga gccccgcccc ggagcgagta cccgccggcc tgagggccgc
     1321 ccccagcgcg agcccgcccc ggatgtctgg gtgtttccct gcgaccgaga cctgccggac
     1381 agcagcgact ctgaggcgga gaccgaagtg ggggggcggg gggacgccga ccaccatgac
     1441 gacgactccg cctccgaggc ggacagcacg gacacggaac tgttcgagac ggggctgctg
     1501 gggccgcagg gcgtggatgg gggggcggtc tcggggggga gccccccccg cgaggaagac
     1561 cccggcagtt gcgggggcgc cccccctcga ggaattccaa ttcggggcaa cacctgcccg
     1621 ctgtgcaacg ccaagctggt gtacctgata gtgggcgtga cgcccagcgg gtcgttcagc
     1681 accatcccga tcgtgaacga cccccagacc cgcatggagg ccgaggaggc cgtcagggcg
     1741 ggcacggccg tggactttat ctggacgggc aatcagcggt tcgccccgcg gtacctgacc
     1801 ctgggggggc acacggtgag ggccctgtcg cccacccacc cggagcccac cacggacgag
     1861 gatgacgacg acctggacga cgcagactac gtaccgcccg ccccccgccg gacgccccgc
     1921 gcccccccac gcagaggcgc cgccgcgccc cccgtgacgg gcggggcgtc tcacgcagcc
     1981 ccccagccgg ccgcggctcg gacagcgccc ccctcggcgc ccatcgggcc acacggcagc
     2041 agtaacacca acaccaccac caacagcagc ggcggcggcg gctcccgcca gtcgcgagcc
     2101 gcggcgccgc ggggggcgtc tggcccctcc gggggggttg gggttggggt tggggttgtt
     2161 gaagcggagg cggggcggcc gaggggccgg acgggccccc ttgtcaacag acccgccccc
     2221 cttgcaaaca acagagaccc catagtgatc agcgactccc ccccggcctc tccccacagg
     2281 ccccccgcgg cgcccatgcc aggctccgcc ccccgccccg ggccccccgc gtccgcggcc
     2341 gcgtcgggac ccgcgcgccc ccgcgcggcc gtggccccgt gcgtgcgagc gccgcctccg
     2401 gggcccggcc cccgcgcccc ggcccccggg gcggagccgg ccgcccgccc cgcggacgcg
     2461 cgccgtgtgc cccagtcgca ctcgtccctg gctcaggccg cgaaccaaga acagagtctg
     2521 tgccgggcgc gtgcgacggt ggcgcgcggc tcgggggggc cgggcgtgga gggtgggcac
     2581 gggccctccc gcggcgccgc cccctccggc gccgccccgc tcccctccgc cgcctctgtc
     2641 gagcaggagg cggcggtgcg tccgaggaag aggcgcgggt cgggccagga aaacccctcc
     2701 ccccagtcca cgcgtccccc cctcgcgccg gcaggggcca agagggcggc gacgcacccc
     2761 ccctccgact cagggccggg ggggcgcggc cagggtgggc ccgggacccc cctgacgtcc
     2821 tcggcggcct ccgcctcttc ctcctctgcc tcttcctcct cggccccgac ccccgcgggg
     2881 gccgcctctt ccgccgccgg ggccgcgtcc tcctccgctt ccgcctcctc gggcggggcc
     2941 gtcggtgccc tgggagggag acaagaggaa acctccctcg gcccccgcgc tgcttctggg
     3001 ccgcgggggc cgaggaagtg tgcccggaag acgcgccacg cggagacttc cggggccgtc
     3061 cccgcgggcg gcctcacgcg ctacctgccc atctcggggg tctctagcgt ggtcgccctg
     3121 tcgccttacg tgaacaagac tatcacgggg gactgcctgc ccatcctgga catggagacg
     3181 gggaacatcg gggcgtacgc ggtcctggtg gaccagacgg gaaacatggc gacccggctg
     3241 cgggccgcgg tccccggctg gagccgccgc accctgctcc ccgagaccgc gggtaaccac
     3301 gtgatgcccc ccgagtaccc gacggccccc gcgtcggagt ggaacagcct ctggatgacc
     3361 cccgtgggga acatgctgtt cgaccagggc accctagtgg gcgccctgga cttccgcagc
     3421 ctgcggtctc ggcacccgtg gtccggggag cagggggcgt cgacccggga cgagggaaaa
     3481 caataagaat tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact
     3541 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3601 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     3661 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3721 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg
     3781 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     3841 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     3901 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     3961 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     4021 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     4081 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4141 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4201 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     4261 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     4381 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4441 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4501 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4561 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4621 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4681 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     4741 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4801 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4861 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4921 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4981 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5041 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5101 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5161 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5221 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5281 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5341 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5401 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5461 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5521 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5581 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5641 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5701 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     5761 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     5821 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     5881 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     5941 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     6001 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6061 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6841 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
     6901 gcccatatat ggagttccg
//
