LOCUS       sequence-26551-e         819 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Howarth M, Takao K, Hayashi Y, Ting AY
  TITLE     Targeting quantum dots to surface proteins in living cells with 
            biotin ligase.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 May 24. 102(21):7583-8.
  PUBMED    15897449
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 May 24. 102(21):7583-8."
COMMENT     Sequence Label: pDisplay-AP-CFP-TM
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     161..223
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             224..250
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             278..322
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(377..398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(638..657)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggngnanta gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag cttggtaccg agctcggatc cactagtaac
      121 ggccgccagt gtgctggaat tcggcttggg gatatccacc atggagacag acacactcct
      181 gctatgggta ctgctgctct gggttccagg ttccactggt gactatccat atgatgttcc
      241 agattatgct ggggcccagc cggccagatc tggcggcggc ctgaacgaca tcttcgaggc
      301 ccagaagatc gagtggcacg agggcgcgcc gatggtgagc aagggcgagg agctgttcac
      361 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca ggttcagcgt
      421 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      481 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      541 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      601 cgaaggctac gtccaggagc gtaccatctt cttcaaggac gacggcaact acaagacccg
      661 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      721 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactnct cagccacaac
      781 gtctatatca ccgccganaa ncaaaaaacg gcatcaggc
//
