LOCUS       sequence-265766-        1000 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Conjugative vector derived from pSHDY. Constitutive expression of 
            the repressor tetR, as well as aTc-inducible expression of mVenus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Behle A, Saake P, Germann AT, Dienst D, Axmann IM
  TITLE     Comparative Dose-Response Analysis of Inducible Promoters in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2020 Apr 17;9(4):843-855. doi: 
            10.1021/acssynbio.9b00505. Epub 2020 Mar 17.
  PUBMED    32134640
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00505"; journalName: "ACS Synth Biol"; date: 
            "2020-04-17- 17"; volume: "9"; issue: "4"; pages: "843-855"
COMMENT     Sequence Label: pSHDY-PL03-mVenus_tetR
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..41
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      880..951
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      967..994
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ggtacgcaat tggcatgcgc attccctatc agtgatagag attgacatcc ctatcagtga
       61 tagatataat ggccacttac tagagtagtg gaggttacta gatggctagc aaaggagagg
      121 aactgtttac cggtgtggta cccattttag tagaattgga tggtgatgtg aacggccaca
      181 agttcagcgt ttccggggaa ggcgaagggg atgcaaccta tggaaagcta actttgaaac
      241 tcatttgcac taccggtaaa ctccccgtcc cctggcctac gttagtcacg accctcggtt
      301 atggtctgca atgttttgcc cgttatcccg atcatatgaa acaacacgat ttctttaaat
      361 ctgccatgcc agagggatat gttcaagaac ggacaatttt ctttaaggat gatggtaatt
      421 ataaaacccg tgcagaagtc aaatttgaag gtgatacact cgtgaatcgc atcgaactaa
      481 aaggtattga ctttaaggag gatggaaaca ttctcggcca taagttagaa tacaattaca
      541 acagccataa tgtgtacatt actgcggata aacaaaaaaa cggtatcaaa gccaacttta
      601 aaattcgaca taacatcgaa gatggtggcg ttcagttagc ggatcactac caacaaaata
      661 ctcccattgg agacggacca gtcctgctgc ctgataacca ttatctgtct tatcaatcca
      721 aactgtccaa ggatccgaat gaaaagcggg atcacatggt tctgttggag tttgtgaccg
      781 cggcaggtat tactctcgga atggacgaac tatacaaaag gcctgccgcc aacgacgaaa
      841 actacgctgc atcagtttaa tagtactaga gccaggcatc aaataaaacg aaaggctcag
      901 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      961 cacactggct caccttcggg tgggcctttc tgcgtttata
//
