LOCUS       sequence-265783-       13422 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses 24xSunTag-2xIFP2-CAAX  (24xGCN4_v4-2xIFP2-CAAX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13422)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 13422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pHRdSV40-UP-24xGCN4-2xIFP2-CAAX-24xPP7
FEATURES             Location/Qualifiers
     source          1..13422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(207..226)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(440..459)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     559..581
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(619..637)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        705..809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(810..878,879..1670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1028..1047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1841..2429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2330..2349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2583..2600
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2670..2690)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2675..3004
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2855..2990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4138..4203
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4333..4353
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4778..4912
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4815..4834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4869..4888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5080..5713
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5760..5885
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6382..6615
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6800..6844
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7129..7246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7309..7519
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7378..7513
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7635..7691
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7707..7763
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7779..7835
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7851..7907
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7923..7979
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7995..8051
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8067..8123
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8139..8195
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8211..8267
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8283..8339
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8355..8411
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8427..8483
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8499..8555
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8571..8627
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8643..8699
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8715..8771
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8787..8843
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8859..8915
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8931..8987
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9003..9059
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9075..9131
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9147..9203
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9291..9347
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9411..10370
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="ARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADG
                     HSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLDW
                     PAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQTV
                     RELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLRL
                     TADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGGQ
                     LWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     CDS             10386..11345
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="ARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADG
                     HSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLDW
                     PAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQTV
                     RELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLRL
                     TADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGGQ
                     LWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     LTR             13063..13296
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(13321..13339)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(13322..13339)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
       61 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
      121 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
      181 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      241 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      301 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      361 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      421 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
      481 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      541 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
      601 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      661 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2581 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2641 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     2701 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     2761 atgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2821 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     2881 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     2941 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3001 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     3061 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     3121 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     3181 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     3241 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     3301 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     3361 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     3421 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     3481 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     3541 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     3601 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     3661 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     3721 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     3781 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     3841 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     3901 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     3961 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     4021 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     4081 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     4141 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     4201 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     4261 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     4321 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     4381 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     4441 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     4501 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     4561 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     4621 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     4681 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     4741 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     4801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     4921 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     4981 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     5041 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     5101 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     5161 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     5221 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     5281 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     5341 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     5401 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     5461 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     5521 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5581 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5641 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5701 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     5761 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     5821 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     5881 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     5941 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     6001 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     6061 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     6121 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     6181 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     6241 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     6301 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     6361 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     6421 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     6481 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     6541 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     6601 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     6661 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     6721 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     6781 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     6841 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     6901 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     6961 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     7021 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     7081 agtgaacgga tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa
     7141 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     7201 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     7261 ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattcaggc aggcagaagt
     7321 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7381 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7441 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     7501 ttttttggag gcctaggcta cgcgtgaatt cgttgcatat ggccaccatg actgcagttg
     7561 gtagctcgga tccgatccat ttggcgcgcc gattactcgg tccgggtgga tctggaggtg
     7621 gaggttctgg aggagaagaa cttttgagca agaattatca tcttgagaac gaagtggctc
     7681 gtcttaagaa aggttctggc agtggagaag aactgctttc aaagaattac cacctggaaa
     7741 atgaggtagc tagactgaaa aaggggagcg gaagtgggga ggagttgctg agcaaaaatt
     7801 atcatttgga gaacgaagta gcacgactaa agaaagggtc cggatcgggt gaggagttac
     7861 tctcgaaaaa ttatcatctc gaaaacgaag tggctcggct aaaaaagggc agtggttctg
     7921 gagaagagct attatctaaa aactaccacc tcgaaaatga ggtggcacgc ttaaaaaagg
     7981 gaagtggcag tggtgaagag ctactatcca agaattatca tcttgagaac gaggtagcgc
     8041 gtttgaagaa gggttccggc tcaggagagg aactgctctc gaagaactat catcttgaaa
     8101 atgaggtcgc tcgattaaaa aagggatcgg gcagtggtga ggaactactt tcaaagaatt
     8161 accacctcga aaacgaagta gctcgattaa agaaaggttc agggtcgggt gaagaattac
     8221 tgagtaaaaa ttatcatctg gaaaatgagg tagcgagact aaaaaagggg agtggttctg
     8281 gcgaagagtt gctatcgaaa aattatcatc ttgagaacga agttgctagg ctcaaaaagg
     8341 gctcaggctc aggcgaggag ttgctctcga aaaactacca cttggaaaat gaggtcgcga
     8401 ggttgaaaaa ggggagcggg tcgggcgagg agttattgag caaaaactat catttagaga
     8461 acgaagtcgc gcgcttaaag aaaggctcgg gctcgggcga agaactctta tcgaagaact
     8521 accacctcga aaatgaggtc gccaggttga aaaagggcag tggcagcggg gaggaactct
     8581 tgagcaagaa ctaccacttg gagaatgagg tcgcgagatt gaagaaaggg tcggggagcg
     8641 gcgaggaatt gctcagcaag aattatcatt tggagaacga agtcgccagg ctcaagaaag
     8701 gctcggggtc gggggaggaa ttgttgagta aaaactacca cttggaaaat gaagtcgcca
     8761 ggctcaaaaa agggagtggg agcggcgaag agttattgag caaaaattac cacttggaga
     8821 acgaagtggc aaggctcaag aaagggagcg gcagcgggga ggagctctta tcgaagaact
     8881 accacttaga gaatgaagtc gcccgcttga agaaaggctc ggggagcggg gaagagctct
     8941 tgagcaagaa ctaccacttg gaaaatgagg tggcgcgctt gaagaaaggg agcgggagcg
     9001 gggaagagtt actatctaag aattatcatc tcgagaacga ggtggctcga ctaaagaagg
     9061 gctccggcag tggggaggaa ctcctgtcga agaactatca tcttgaaaat gaggttgcaa
     9121 gacttaaaaa ggggtccgga tcaggtgagg aactactcag taagaattac cacctggaaa
     9181 acgaagttgc acgtttgaag aaaggatcag gatcaggcga agaactgctc tcaaaagatt
     9241 atcatttgga aaatgaggtt gcacgtttaa aaaagggaag tggcagtggt gaggaacttc
     9301 tgtcgaaaaa ttatcatctc gagaatgaag tagcccgact taaaaagggt tctggctcgg
     9361 gtcagcggcc gcaccggtac cccgcgggtg gtggtggtag cggtggtggt gctcgggacc
     9421 ctcaaccttt cttccctcct ctgtatctcg gaggaccaga gatcactact gagaactgcg
     9481 agcgggaacc tatacatata ccaggttcta ttcaacctca tggagcactg ctcacagccg
     9541 atggacattc tggcgaggtg ctgcaagttt cactcaacgc agccactttc ctgggccagg
     9601 aaccaactgt tctgcgggga cagaccctgg cagccctgct gccagaccag tggcctgctc
     9661 tgcagactgc cctgcctcca ggctgccagg acgccctgca gtatcgcgcc accctggatt
     9721 ggcccgccgc cggacacctg agcctgaccg tgcatagggt ggccgagctg ctgattctgg
     9781 agttcgagcc tactgaggca tgggactcta ttggacccca cgctctgaga aatgcaatgt
     9841 tcgctctgga gagtgctcct aatctccggg cactggctga agtcgcaacc caaacagtcc
     9901 gggaactgtc aggtttcgac cgggtgatgc tgtacaagtt tgcaccagac gcaacaggag
     9961 aggttattgc cgaagcaagg cgcgagggca tgcaggctta cctcgggcat aggtttcccg
    10021 catccaccac ccctgcacaa gctagggccc tctacacaag acacctgctc cggctgaccg
    10081 cagacaccag ggctgcagca gtgcccctcg accccgtgct gaatccccag acaaatgctc
    10141 ctacacctct gggcggagct gtcctcagag ctacatcccc aatgcacatg cagtacctga
    10201 ggaatatggg agtgggctcc tccctgagcg tcagcgtcgt ggtcggcggc cagctgtggg
    10261 gactgattgt ctgccaccat cagacaccct acgtgctgcc accagatctg cggaccaccc
    10321 tggagtatct ggggaggctc ctgtccctgc aggtgcagag gaaagaagcc cgtacgggag
    10381 gtggtgctcg ggaccctcaa cctttcttcc ctcctctgta tctcggagga ccagagatca
    10441 ctactgagaa ctgcgagcgg gaacctatac atataccagg ttctattcaa cctcatggag
    10501 cactgctcac agccgatgga cattctggcg aggtgctgca agtttcactc aacgcagcca
    10561 ctttcctggg ccaggaacca actgttctgc ggggacagac cctggcagcc ctgctgccag
    10621 accagtggcc tgctctgcag actgccctgc ctccaggctg ccaggacgcc ctgcagtatc
    10681 gcgccaccct ggattggccc gccgccggac acctgagcct gaccgtgcat agggtggccg
    10741 agctgctgat tctggagttc gagcctactg aggcatggga ctctattgga ccccacgctc
    10801 tgagaaatgc aatgttcgct ctggagagtg ctcctaatct ccgggcactg gctgaagtcg
    10861 caacccaaac agtccgggaa ctgtcaggtt tcgaccgggt gatgctgtac aagtttgcac
    10921 cagacgcaac aggagaggtt attgccgaag caaggcgcga gggcatgcag gcttacctcg
    10981 ggcataggtt tcccgcatcc accacccctg cacaagctag ggccctctac acaagacacc
    11041 tgctccggct gaccgcagac accagggctg cagcagtgcc cctcgacccc gtgctgaatc
    11101 cccagacaaa tgctcctaca cctctgggcg gagctgtcct cagagctaca tccccaatgc
    11161 acatgcagta cctgaggaat atgggagtgg gctcctccct gagcgtcagc gtcgtggtcg
    11221 gcggccagct gtggggactg attgtctgcc accatcagac accctacgtg ctgccaccag
    11281 atctgcggac caccctggag tatctgggga ggctcctgtc cctgcaggtg cagaggaaag
    11341 aagccggagg tagcggcgca ggaggtgcag gaagcggtgc tgagcccggt ggttctggtg
    11401 gcaagaagaa aaagaagaag agcaagacca agtgcgtgat catgtaaact agtcctgcag
    11461 gtttaaacga attcgccctt cgcgggatcc taaggtacct aattgcctag aaaggagcag
    11521 acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc
    11581 tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca
    11641 gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg
    11701 ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc
    11761 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc
    11821 gctggctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag
    11881 cagacgatat ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct
    11941 cgctggctgc agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga
    12001 gcagacgata tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc
    12061 tcgctggctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg
    12121 agcagacgat atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg
    12181 ctcgctggct gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag
    12241 gagcagacga tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg
    12301 gctcgctggc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
    12361 ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg
    12421 ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa
    12481 aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat
    12541 gggctcgctg gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga
    12601 aaggagcaga cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata
    12661 tgggctcgct ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag
    12721 aaaggagcag acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat
    12781 atgggctcgc tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta
    12841 gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca
    12901 tatgggctcg ctggctgcag tattcccggg ttcattagat ctcgcgaagg gcgaattcgc
    12961 ggccgcgatc atattcgaag atcttaatta aggtaccttt aagaccaatg acttacaagg
    13021 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
    13081 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
    13141 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    13201 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    13261 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
    13321 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
    13381 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tc
//
