LOCUS       sequence-265868-        9272 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses LbCas12a RVRR variant in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Toth E, Varga E, Kulcsar PI, Kocsis-Jutka V, Krausz SL, Nyeste A, 
            Welker Z, Huszar K, Ligeti Z, Talas A, Welker E
  TITLE     Improved LbCas12a variants with altered PAM specificities further 
            broaden the genome targeting range of Cas12a nucleases.
  JOURNAL   Nucleic Acids Res. 2020 Feb 28. pii: 5763098. doi: 
            10.1093/nar/gkaa110.
  PUBMED    32107556
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Feb 28. pii: 5763098. doi: 10.1093/nar/gkaa110."
COMMENT     Sequence Label: pTE3646
FEATURES             Location/Qualifiers
     source          1..9272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        144..523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        524..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     678..698
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     772..791
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        772..790
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      826..835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             838..858
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             883..4563
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMRGWDKDVETDRRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPRVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             4564..4611
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4618..4698
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4772..4789)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4778..5002
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5048..5476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5135..5154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5345..5366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5485..5505)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5490..5819
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5670..5805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5732..5751
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5886..6680
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5940..5959)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6550..6569
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6854..6975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6891..6910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6945..6964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7024..7040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7024..7040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7037..7059)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7048..7064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7072..7078,7079..7096,7097..7102))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7117..7138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7255..7272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7426..8014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7506..7525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8185..8976,8977..9045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8808..8827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9046..9150)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9225..9244)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
       61 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
      121 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      181 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      241 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      301 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      361 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      421 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      481 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      541 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      601 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
      781 tcactatagg gagacccaag ctggctagcg tttaaactta agcttgccac catggcccca
      841 aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccagcaagct ggagaagttt
      901 acaaactgct actccctgtc taagaccctg aggttcaagg ccatccctgt gggcaagacc
      961 caggagaaca tcgacaataa gcggctgctg gtggaggacg agaagagagc cgaggattat
     1021 aagggcgtga agaagctgct ggatcgctac tatctgtctt ttatcaacga cgtgctgcac
     1081 agcatcaagc tgaagaatct gaacaattac atcagcctgt tccggaagaa aaccagaacc
     1141 gagaaggaga ataaggagct ggagaacctg gagatcaatc tgcggaagga gatcgccaag
     1201 gccttcaagg gcaacgaggg ctacaagtcc ctgtttaaga aggatatcat cgagacaatc
     1261 ctgccagagt tcctggacga taaggacgag atcgccctgg tgaacagctt caatggcttt
     1321 accacagcct tcaccggctt ctttgataac agagagaata tgttttccga ggaggccaag
     1381 agcacatcca tcgccttcag gtgtatcaac gagaatctga cccgctacat ctctaatatg
     1441 gacatcttcg agaaggtgga cgccatcttt gataagcacg aggtgcagga gatcaaggag
     1501 aagatcctga acagcgacta tgatgtggag gatttctttg agggcgagtt ctttaacttt
     1561 gtgctgacac aggagggcat cgacgtgtat aacgccatca tcggcggctt cgtgaccgag
     1621 agcggcgaga agatcaaggg cctgaacgag tacatcaacc tgtataatca gaaaaccaag
     1681 cagaagctgc ctaagtttaa gccactgtat aagcaggtgc tgagcgatcg ggagtctctg
     1741 agcttctacg gcgagggcta tacatccgat gaggaggtgc tggaggtgtt tagaaacacc
     1801 ctgaacaaga acagcgagat cttcagctcc atcaagaagc tggagaagct gttcaagaat
     1861 tttgacgagt actctagcgc cggcatcttt gtgaagaacg gccccgccat cagcacaatc
     1921 tccaaggata tcttcggcga gtggaacgtg atccgggaca agtggaatgc cgagtatgac
     1981 gatatccacc tgaagaagaa ggccgtggtg accgagaagt acgaggacga tcggagaaag
     2041 tccttcaaga agatcggctc cttttctctg gagcagctgc aggagtacgc cgacgccgat
     2101 ctgtctgtgg tggagaagct gaaggagatc atcatccaga aggtggatga gatctacaag
     2161 gtgtatggct cctctgagaa gctgttcgac gccgattttg tgctggagaa gagcctgaag
     2221 aagaacgacg ccgtggtggc catcatgaag gacctgctgg attctgtgaa gagcttcgag
     2281 aattacatca aggccttctt tggcgagggc aaggagacaa acagggacga gtccttctat
     2341 ggcgattttg tgctggccta cgacatcctg ctgaaggtgg accacatcta cgatgccatc
     2401 cgcaattatg tgacccagaa gccctactct aaggataagt tcaagctgta ttttcagaac
     2461 cctcagttca tgaggggctg ggacaaggat gtggagaccg atcggcgggc caccatcctg
     2521 agatacggct ccaagtacta tctggccatc atggataaga agtacgccaa gtgcctgcag
     2581 aagatcgaca aggacgatgt gaacggcaat tacgagaaga tcaactataa gctgctgccc
     2641 ggccctaata agatgctgcc aagggtgttc ttttctaaga agtggatggc ctactataac
     2701 cccagcgagg acatccagaa gatctacaag aatggcacat tcaagaaggg cgatatgttt
     2761 aacctgaatg actgtcacaa gctgatcgac ttctttaagg atagcatctc ccggtatcca
     2821 aagtggtcca atgcctacga tttcaacttt tctgagacag agaagtataa ggacatcgcc
     2881 ggcttttaca gagaggtgga ggagcagggc tataaggtga gcttcgagtc tgccagcaag
     2941 aaggaggtgg ataagctggt ggaggagggc aagctgtata tgttccagat ctataacaag
     3001 gacttttccg ataagtctca cggcacaccc aatctgcaca ccatgtactt caagctgctg
     3061 tttgacgaga acaatcacgg acagatcagg ctgagcggag gagcagagct gttcatgagg
     3121 cgcgcctccc tgaagaagga ggagctggtg gtgcacccag ccaactcccc tatcgccaac
     3181 aagaatccag ataatcccaa gaaaaccaca accctgtcct acgacgtgta taaggataag
     3241 aggttttctg aggaccagta cgagctgcac atcccaatcg ccatcaataa gtgccccaag
     3301 aacatcttca agatcaatac agaggtgcgc gtgctgctga agcacgacga taacccctat
     3361 gtgatcggca tcgatagggg cgagcgcaat ctgctgtata tcgtggtggt ggacggcaag
     3421 ggcaacatcg tggagcagta ttccctgaac gagatcatca acaacttcaa cggcatcagg
     3481 atcaagacag attaccactc tctgctggac aagaaggaga aggagaggtt cgaggcccgc
     3541 cagaactgga cctccatcga gaatatcaag gagctgaagg ccggctatat ctctcaggtg
     3601 gtgcacaaga tctgcgagct ggtggagaag tacgatgccg tgatcgccct ggaggacctg
     3661 aactctggct ttaagaatag ccgcgtgaag gtggagaagc aggtgtatca gaagttcgag
     3721 aagatgctga tcgataagct gaactacatg gtggacaaga agtctaatcc ttgtgcaaca
     3781 ggcggcgccc tgaagggcta tcagatcacc aataagttcg agagctttaa gtccatgtct
     3841 acccagaacg gcttcatctt ttacatccct gcctggctga catccaagat cgatccatct
     3901 accggctttg tgaacctgct gaaaaccaag tataccagca tcgccgattc caagaagttc
     3961 atcagctcct ttgacaggat catgtacgtg cccgaggagg atctgttcga gtttgccctg
     4021 gactataaga acttctctcg cacagacgcc gattacatca agaagtggaa gctgtactcc
     4081 tacggcaacc ggatcagaat cttccggaat cctaagaaga acaacgtgtt cgactgggag
     4141 gaggtgtgcc tgaccagcgc ctataaggag ctgttcaaca agtacggcat caattatcag
     4201 cagggcgata tcagagccct gctgtgcgag cagtccgaca aggccttcta ctctagcttt
     4261 atggccctga tgagcctgat gctgcagatg cggaacagca tcacaggccg caccgacgtg
     4321 gattttctga tcagccctgt gaagaactcc gacggcatct tctacgatag ccggaactat
     4381 gaggcccagg agaatgccat cctgccaaag aacgccgacg ccaatggcgc ctataacatc
     4441 gccagaaagg tgctgtgggc catcggccag ttcaagaagg ccgaggacga gaagctggat
     4501 aaggtgaaga tcgccatctc taacaaggag tggctggagt acgcccagac cagcgtgaag
     4561 cacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac
     4621 ccatacgatg ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat
     4681 gatgtccccg actatgccta agaattctgc agatatccag cacagtggcg gccgctcgag
     4741 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4801 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4861 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4921 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4981 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     5041 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5101 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5161 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5221 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5281 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5341 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5401 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5461 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     5521 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     5581 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5641 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5701 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     5761 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5821 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     5881 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5941 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6001 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6061 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     6121 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6181 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6241 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6301 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6361 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     6421 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     6481 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     6541 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     6601 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     6661 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     6721 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6781 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6841 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6901 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6961 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7021 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7081 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7141 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7201 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7321 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7861 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8101 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9181 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     9241 gccgcatagt taagccagta tctgctccct gc
//
