LOCUS       sequence-265877-       10044 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Mohler PJ, Gramolini AO, Bennett V
  TITLE     The ankyrin-B C-terminal domain determines activity of ankyrin-B/G 
            chimeras in rescue of abnormal inositol 1,4,5-trisphosphate and 
            ryanodine receptor distribution in ankyrin-B (-/-) neonatal 
            cardiomyocytes.
  JOURNAL   J Biol Chem. 2002 Mar 22. 277(12):10599-607.
  PUBMED    11781319
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Mar 22. 277(12):10599-607."
COMMENT     Sequence Label: AnkyrinG-190-GFP
FEATURES             Location/Qualifiers
     source          1..10044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5984..5993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5990..5992,5993..5995,5996..6709)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5990..5992,5993..5995,5996..6706)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6035..6056)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6296..6315)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6643..6664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6832..6953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6869..6888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6923..6942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6960..7415)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7097..7118)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7309..7328
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7442..7546
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7548..7905
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7548..7739
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7571..7591)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7756..7891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7940..8734
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7940..8734
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7994..8013)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8604..8623
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8922..8941)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8966..9013
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9342..9930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9831..9850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcatggct catgccgcct cccagttaaa
      661 gaaaaacagg gatttagaaa tcaatgcgga agaagagacc gagaaaaaaa agaagcaccg
      721 taaacggtcc cgggatcgca agaaaaagtc ggatgccaat gcaagttact tgagagcagc
      781 tcgggcgggg cacctggaaa aggcccttga ctacatcaaa aatggagtgg acgtcaacat
      841 ctgtaaccag aatgggttga atgcgctcca tcttgcttcc aaagaaggcc atgtggaagt
      901 ggtctccgag ctgctgcaga gagaagccaa cgtcgatgca gccacaaaga aaggaaacac
      961 agccttacac atcgcatcct tggctggaca agcggaagtg gtcaaggtct tggttacgaa
     1021 cggagcgaac gtcaacgcac agtctcagaa tggcttcacg ccgctgtata tggcggccca
     1081 ggagaaccac ctggaagtcg tcaggtttct tctggacaat ggtgccagcc aaagcctggc
     1141 tacagaggat ggcttcacgc cattggccgt ggctcttcaa caaggtcacg accaagtcgt
     1201 atccctcctg ctcgagaacg acacgaaggg aaaagtgcgc ctcccagccc tccacatcgc
     1261 ggcccggaaa gatgacacca aagcggcagc tctgctccta cagaacgaca ccaatgcaga
     1321 catagagtca aagatggtgg tgaatagagc aactgagagt ggcttcacct cactccacat
     1381 agctgcccac tacgggaata tcaatgtggc cacgttgctg ttaaaccgag cggctgccgt
     1441 ggacttcacc gcacggaatg acatcactcc tttgcatgtt gcctcgaaac ggggaaacgc
     1501 aaatatggta aagctgttgc tcgaccgggg tgcgaagatc gacgccaaga ccagggacgg
     1561 tctgaccccg ctgcactgcg gggcgagaag tggccacgag caggtggtag agatgttgct
     1621 ggacagagcc gcccccatcc tttcgaaaac caagaatgga ctgtccccac tgcacatggc
     1681 cacacaagga gaccatttaa actgcgtcca actcctcctc cagcacaacg tgcccgtgga
     1741 tgacgtcacc aacgactacc tgaccgccct ccacgtggcg gcccactgcg gccattacaa
     1801 agttgccaag gttcttttgg ataagaaagc taaccccaat gccaaagccc tgaatggctt
     1861 cacccctctc catatcgcct gcaaaaagaa ccgaatccga gtaatggaac tccttttgaa
     1921 gcacggtgca tctattcaag ccgtaaccga gtcgggcctt accccaatcc atgtcgctgc
     1981 cttcatggga catgtaaata tcgtgtcaca gctaatgcat catggagcct ccccaaacac
     2041 caccaacgtg agaggagaaa cagcattgca tatggcggct cggtcggggc aagcagaagt
     2101 ggtgcggtat ttggtacaag atggagctca ggtagaagca aaagctaagg atgaccagac
     2161 cccactccac atttcagccc gactcgggaa ggctgacata gtgcaacaac tgttacagca
     2221 aggagcgtcc ccgaatgcag caacaacttc cgggtacacc ccccttcacc tttcggccag
     2281 agaggggcat gaggatgtag ctgccttcct tctggaccat ggagcatctt tatccataac
     2341 aacaaagaag ggattcacgc ctctgcatgt ggctgcaaag tatgggaagc ttgaagtggc
     2401 aagtctcctg ctgcagaaga gtgcgtctcc tgatgccgct gggaagagcg ggctaactcc
     2461 actgcatgta gcagcacatt acgataatca gaaagtggcc cttctgcttt tggaccaggg
     2521 agcctcaccc cacgcagctg caaagaacgg ttatacgcca ctgcacatcg ccgccaagaa
     2581 gaaccagatg gacatagcca cgtccctgct ggagtacggt gctgatgcaa acgccgttac
     2641 ccggcaaggg attgcgtccg tccaccttgc agcgcaggaa gggcacgtgg acatggtgtc
     2701 gctgctcctc agtagaaatg cgaacgtcaa cttgagcaat aagagcggcc tcaccccact
     2761 ccacctggcg gctcaagaag accgagtgaa cgtggctgag gtccttgtca accaaggggc
     2821 ccacgtggat gcacagacaa agatgggcta cacgccgctc catgtgggct gtcactatgg
     2881 aaacatcaaa atagtcaatt ttctgctgca gcattctgcg aaagttaatg ccaagacaaa
     2941 gaatggatac acgccactgc accaggcagc ccagcagggc cacacacaca tcatcaacgt
     3001 cctgctccag aacaacgctt cccccaatga actcactgtg aatgggaaca cagccctggc
     3061 catcgcccga cgccttggct acatctcggt ggttgacacc ctaaaggtcg tgacagagga
     3121 aattatgacc accactacca tcacggagaa gcacaaaatg aatgtcccag aaacaatgaa
     3181 tgaagtcctc gacatgtcag acgatgaagt tggtaaagcc agcgcccccg aaaagctcag
     3241 tgatggggaa tatatctcag atggtgaaga aggtgaagat gccatcacag gggacactga
     3301 caagtatctc gggccacagg acctcaagga gctgggcgat gactccctgc ctgccgaggg
     3361 ctatgtgggc ttcagtctcg gagcccgttc tgccagcctt cgctccttca gttcggatag
     3421 gtcctacacc ctgaacagaa gctcctatgc gcgggacagc atgatgatcg aggagcttct
     3481 ggtgccatcc aaagagcagc accttccatt cacgagggag tttgactccg actccctcag
     3541 acactacagt tgggcagcag acacactgga caatgtgaac ctggtctcca gccctgtgca
     3601 ttctgggttt ctggttagct ttatggtgga cgcgagaggg ggctccatgc gaggaagccg
     3661 ccaccacggg atgcggatca tcatccctcc gcgcaagtgt acggccccca cccgcatcac
     3721 ttgccgcctg gtaaagagac ataaactggc caacccaccc cccatggtgg aaggagaggg
     3781 attagccagt aggctggtag aaatgggtcc tgcgggggca caatttttag gcccggtcat
     3841 agtggaaatc cctcattttg ggtccatgag aggaaaggag agggaactca ttgtcctccg
     3901 gagcgagaac ggagagacct ggaaggaaca ccagtttgac agcaaaaacg aagacctctc
     3961 ggagctcctc aatggcatgg acgaagaact tgacagccca gaagagttgg gcacaaagcg
     4021 catctgcaga atcatcacga aggatttccc ccagtatttc gctgtggttt cccggattaa
     4081 gcaggaaagc aaccagattg gtcccgaagg tgggattctg agcagcacca cggtccccct
     4141 cgtccaggcg tctttcccag agggtgcctt aaccaaaagg atccgtgtgg gtctgcaggc
     4201 tcagcccgta ccagaggaga ctgtgaaaaa gatccttgga aacaaagcaa cgtttagccc
     4261 aattgtcacg gtggagccga ggaggaggaa attccataag ccgatcacca tgaccatccc
     4321 ggtgccccca ccctcgggag aaggcgtgtc caatgggtac aagggggaca ccacgcccag
     4381 cctgcgactc ctctgcagca ttacaggagg gacctcgccg gctcagtggg aagacatcac
     4441 aggcacaacc ccgctgacct tcataaagga ctgtgtgtct ttcacaacca atgtttcagc
     4501 caggttctgg ctggcggact gccatcaggt gttagaaacc gtagggctag cctcgcagct
     4561 gtacagagag ttgatatgtg tcccctacat ggccaagttt gttgtttttg ccaaaacaaa
     4621 cgacccagtg gaatcctccc taaggtgctt ctgtatgaca gacgacagag tggacaaaac
     4681 cctggagcag caggagaatt tcgaggaggt tgccagaagc aaagacatcg aggttctgga
     4741 aggaaagccc atctatgttg attgctatgg aaacctggct cctctgacca aaggagggca
     4801 gcagcttgtt tttaacttct attctttcaa agaaaacaga ctgccatttt ccatcaaggt
     4861 tagagacacc agccaagagc cctgcggccg tctgtctttc ctgaaggagc cgaaaacaac
     4921 aaaaggatta ccccaaacgg ctgtttgcaa cttaaatatt actctaccgg cgcataaaaa
     4981 ggctgagaag gcagacagac gccagagctt cacatcccta gccttacgta agcgctacag
     5041 ctacttgact gagcccagca tgagtccaca gagtccttgt gagcggacgg atatcaggat
     5101 ggcaatagta gccgatcacc tgggacttag ttggacagag ctggcaaggg aactgaattt
     5161 ttcagtggat gaaatcaacc aaatacgagt ggaaaatccc aattctttaa tttctcagag
     5221 cttcatgtta ttaaaaaagt gggtgacgag agacgggaag aatgccacaa ctgatgcctt
     5281 aacttcggtc ttaacaaaaa ttaaccgaat agacatagta actctgctgg aaggaccaat
     5341 atttgattat gggaatattt caggcaccag aagctttgca gatgaaaaca atgttttcca
     5401 tgacccagtt gatggttggc agaacgagac gccaagtgga agcctagagt ccccggcgca
     5461 agcgcgaaga ataaccggtg ggttactgga ccgtctggac gacagctctg accaggttcg
     5521 ggatcccatt acctcatacc tcacaggaga agctgggaag ttcgaagcaa acggaaacca
     5581 cgcagaagtc attccagaag caaaggccaa ggcctacttt cctgaatccc aaaacgatat
     5641 agggaaacag agcatcaagg agaacttgaa accaaaaaca cacggatgtg gtcgcgctga
     5701 ggaaccagtg tcgcccctca cagcctacca gaaatctcta gaagaaacca gcaagcttgt
     5761 catagaagac gcacctaaac cctgtgtgcc tgtcggcatg aaaaagatga ccaggactcc
     5821 ggctgacggc aaagccaggc tcaacctcca ggaagaggag gggtccgcca ggtcagagcc
     5881 taagcaggga gaaggctata aggtgaaaac aaagaaggaa atccggaatg tggagaagaa
     5941 agcccaccag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa
     6001 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     6061 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     6121 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     6181 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     6241 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     6301 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     6361 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     6421 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     6481 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     6541 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     6601 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     6661 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga
     6721 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     6781 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     6841 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6901 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta
     6961 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     7021 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     7081 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     7141 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     7201 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     7261 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     7321 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     7381 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     7441 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7501 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     7561 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7621 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7681 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7741 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7801 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7861 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     7921 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     7981 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     8041 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     8101 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     8161 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     8221 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     8281 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     8341 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     8401 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     8461 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     8521 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     8581 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     8641 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     8701 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     8761 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     8821 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     8881 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     8941 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     9001 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     9061 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     9121 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     9181 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9241 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9961 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10021 gataaccgta ttaccgccat gcat
//
