LOCUS       sequence-265878-        9272 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses impLbCas12a in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Toth E, Varga E, Kulcsar PI, Kocsis-Jutka V, Krausz SL, Nyeste A, 
            Welker Z, Huszar K, Ligeti Z, Talas A, Welker E
  TITLE     Improved LbCas12a variants with altered PAM specificities further 
            broaden the genome targeting range of Cas12a nucleases.
  JOURNAL   Nucleic Acids Res. 2020 Feb 28. pii: 5763098. doi: 
            10.1093/nar/gkaa110.
  PUBMED    32107556
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Feb 28. pii: 5763098. doi: 10.1093/nar/gkaa110."
COMMENT     Sequence Label: pVE13300
FEATURES             Location/Qualifiers
     source          1..9272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(118..909,910..978))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     741..760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(979..1083)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1158..1177)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1349..1728
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1729..1932
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1883..1903
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1977..1996
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1977..1995
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2031..2040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2043..2063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2088..5768
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMRGWDKDVETDRRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPRVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             5769..5816
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5823..5903
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5977..5994)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5983..6207
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6253..6681
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6340..6359)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6550..6571
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6690..6710)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6695..7024
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6875..7010
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6937..6956
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7091..7885
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7145..7164)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7755..7774
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8059..8180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8096..8115)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8150..8169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8229..8245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8229..8245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8242..8264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8253..8269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8277..8283,8284..8301,8302..8307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8322..8343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8460..8477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8631..9219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8711..8730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgttaaggg attttggtca ccatggtatc aaaaaggatc ttcacctaga tccttttaaa
       61 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      121 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      181 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      241 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      301 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      361 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      421 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      481 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      541 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
      601 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      661 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      721 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      781 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      841 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      901 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      961 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1021 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1081 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     1141 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     1201 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     1261 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     1321 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     1381 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1441 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     1501 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     1561 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     1621 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     1681 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     1741 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     1801 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     1861 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     1921 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     1981 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt gccaccatgg
     2041 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccagc aagctggaga
     2101 agtttacaaa ctgctactcc ctgtctaaga ccctgaggtt caaggccatc cctgtgggca
     2161 agacccagga gaacatcgac aataagcggc tgctggtgga ggacgagaag agagccgagg
     2221 attataaggg cgtgaagaag ctgctggatc gctactatct gtcttttatc aacgacgtgc
     2281 tgcacagcat caagctgaag aatctgaaca attacatcag cctgttccgg aagaaaacca
     2341 gaaccgagaa ggagaataag gagctggaga acctggagat caatctgcgg aaggagatcg
     2401 ccaaggcctt caagggcaac gagggctaca agtccctgtt taagaaggat atcatcgaga
     2461 caatcctgcc agagttcctg gacgataagg acgagatcgc cctggtgaac agcttcaatg
     2521 gctttaccac agccttcacc ggcttctttc gtaacagaga gaatatgttt tccgaggagg
     2581 ccaagagcac atccatcgcc ttcaggtgta tcaacgagaa tctgacccgc tacatctcta
     2641 atatggacat cttcgagaag gtggacgcca tctttgataa gcacgaggtg caggagatca
     2701 aggagaagat cctgaacagc gactatgatg tggaggattt ctttgagggc gagttcttta
     2761 actttgtgct gacacaggag ggcatcgacg tgtataacgc catcatcggc ggcttcgtga
     2821 ccgagagcgg cgagaagatc aagggcctga acgagtacat caacctgtat aatcagaaaa
     2881 ccaagcagaa gctgcctaag tttaagccac tgtataagca ggtgctgagc gatcgggagt
     2941 ctctgagctt ctacggcgag ggctatacat ccgatgagga ggtgctggag gtgtttagaa
     3001 acaccctgaa caagaacagc gagatcttca gctccatcaa gaagctggag aagctgttca
     3061 agaattttga cgagtactct agcgccggca tctttgtgaa gaacggcccc gccatcagca
     3121 caatctccaa ggatatcttc ggcgagtgga acgtgatccg ggacaagtgg aatgccgagt
     3181 atgacgatat ccacctgaag aagaaggccg tggtgaccga gaagtacgag gacgatcgga
     3241 gaaagtcctt caagaagatc ggctcctttt ctctggagca gctgcaggag tacgccgacg
     3301 ccgatctgtc tgtggtggag aagctgaagg agatcatcat ccagaaggtg gatgagatct
     3361 acaaggtgta tggctcctct gagaagctgt tcgacgccga ttttgtgctg gagaagagcc
     3421 tgaagaagaa cgacgccgtg gtggccatca tgaaggacct gctggattct gtgaagagct
     3481 tcgagaatta catcaaggcc ttctttggcg agggcaagga gacaaacagg gacgagtcct
     3541 tctatggcga ttttgtgctg gcctacgaca tcctgctgaa ggtggaccac atctacgatg
     3601 ccatccgcaa ttatgtgacc cagaagccct actctaagga taagttcaag ctgtattttc
     3661 agaaccctca gttcatgagg ggctgggaca aggatgtgga gaccgatcgg cgggccacca
     3721 tcctgagata cggctccaag tactatctgg ccatcatgga taagaagtac gccaagtgcc
     3781 tgcagaagat cgacaaggac gatgtgaacg gcaattacga gaagatcaac tataagctgc
     3841 tgcccggccc taataagatg ctgccaaggg tgttcttttc taagaagtgg atggcctact
     3901 ataaccccag cgaggacatc cagaagatct acaagaatgg cacattcaag aagggcgata
     3961 tgtttaacct gaatgactgt cacaagctga tcgacttctt taaggatagc atctcccggt
     4021 atccaaagtg gtccaatgcc tacgatttca acttttctga gacagagaag tataaggaca
     4081 tcgccggctt ttacagagag gtggaggagc agggctataa ggtgagcttc gagtctgcca
     4141 gcaagaagga ggtggataag ctggtggagg agggcaagct gtatatgttc cagatctata
     4201 acaaggactt ttccgataag tctcacggca cacccaatct gcacaccatg tacttcaagc
     4261 tgctgtttga cgagaacaat cacggacaga tcaggctgag cggaggagca gagctgttca
     4321 tgaggcgcgc ctccctgaag aaggaggagc tggtggtgca cccagccaac tcccctatcg
     4381 ccaacaagaa tccagataat cccaagaaaa ccacaaccct gtcctacgac gtgtataagg
     4441 ataagaggtt ttctgaggac cagtacgagc tgcacatccc aatcgccatc aataagtgcc
     4501 ccaagaacat cttcaagatc aatacagagg tgcgcgtgct gctgaagcac gacgataacc
     4561 cctatgtgat cggcatcgat aggggcgagc gcaatctgct gtatatcgtg gtggtggacg
     4621 gcaagggcaa catcgtggag cagtattccc tgaacgagat catcaacaac ttcaacggca
     4681 tcaggatcaa gacagattac cactctctgc tggacaagaa ggagaaggag aggttcgagg
     4741 cccgccagaa ctggacctcc atcgagaata tcaaggagct gaaggccggc tatatctctc
     4801 aggtggtgca caagatctgc gagctggtgg agaagtacga tgccgtgatc gccctggagg
     4861 acctgaactc tggctttaag aatagccgcg tgaaggtgga gaagcaggtg tatcagaagt
     4921 tcgagaagat gctgatcgat aagctgaact acatggtgga caagaagtct aatccttgtg
     4981 caacaggcgg cgccctgaag ggctatcaga tcaccaataa gttcgagagc tttaagtcca
     5041 tgtctaccca gaacggcttc atcttttaca tccctgcctg gctgacatcc aagatcgatc
     5101 catctaccgg ctttgtgaac ctgctgaaaa ccaagtatac cagcatcgcc gattccaaga
     5161 agttcatcag ctcctttgac aggatcatgt acgtgcccga ggaggatctg ttcgagtttg
     5221 ccctggacta taagaacttc tctcgcacag acgccgatta catcaagaag tggaagctgt
     5281 actcctacgg caaccggatc agaatcttcc ggaatcctaa gaagaacaac gtgttcgact
     5341 gggaggaggt gtgcctgacc agcgcctata aggagctgtt caacaagtac ggcatcaatt
     5401 atcagcaggg cgatatcaga gccctgctgt gcgagcagtc cgacaaggcc ttctactcta
     5461 gctttatggc cctgatgagc ctgatgctgc agatgcggaa cagcatcaca ggccgcaccg
     5521 acgtggattt tctgatcagc cctgtgaaga actccgacgg catcttctac gatagccgga
     5581 actatgaggc ccaggagaat gccatcctgc caaagaacgc cgacgccaat ggcgcctata
     5641 acatcgccag aaaggtgctg tgggccatcg gccagttcaa gaaggccgag gacgagaagc
     5701 tggataaggt gaagatcgcc atctctaaca aggagtggct ggagtacgcc cagaccagcg
     5761 tgaagcacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat
     5821 cctacccata cgatgttcca gattacgctt atccctacga cgtgcctgat tatgcatacc
     5881 catatgatgt ccccgactat gcctaagaat tctgcagata tccagcacag tggcggccgc
     5941 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     6001 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6061 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6121 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     6181 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     6241 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6301 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6361 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6421 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6481 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6541 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6601 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6661 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6721 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6781 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6841 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6901 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6961 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7021 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     7081 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     7141 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     7201 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     7261 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     7321 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     7381 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     7441 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     7501 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7561 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     7621 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7681 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     7741 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     7801 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     7861 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     7921 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     7981 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8041 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8101 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8161 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     8221 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8281 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8341 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8401 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8461 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8521 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8581 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8641 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8701 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8761 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8821 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8881 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8941 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9001 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9061 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9121 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9181 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9241 acggggtctg acgctcagtg gaacgaaaac tc
//
