LOCUS       sequence-266181-        1561 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  A photoactivatable GEPI variant originated from ecliptic pHluorin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Lee S, Song YK, Baker BJ
  TITLE     Engineering Photoactivatability in Genetically Encoded Voltage and 
            pH Indicators.
  JOURNAL   Front Cell Neurosci. 2019 Oct 29;13:482. doi: 
            10.3389/fncel.2019.00482. eCollection 2019.
  PUBMED    31736711
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Neurosci."; date: "2019-10-29"; volume: "13:482. doi"; pages: " 
            10.3389/fncel.2019.00482. eCollection 2019"
COMMENT     Sequence Label: PA-Bongwoori-R3
FEATURES             Location/Qualifiers
     source          1..1561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             768..1484
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Miesenbock et al., 1998)"
                     /label=ecliptic pHluorin
                     /note="responds to a drop in pH with a gradual loss 
                     (eclipsing) of excitation at 475 nm, accompanied by a 
                     reduction of excitation at 395 nm"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFHTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     complement(798..826)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1421..1441
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1529..1546)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 taatacgact cactataggg agacccaagc tggctagcac catggaggga ttcgacggtt
       61 cagattttag tcctccagct gatttagttg gcgttgacgg tgcagtcatg cggaacgtcg
      121 ttgacgtcac gataaatggt gacgtcactg ctccgccgaa agctgcgcca agaaaaagtg
      181 aatcggtaaa gaaagttcat tggaatgatg tagaccaagg accgagtgaa aaaccagaga
      241 caagacagga ggaacgaata gatatacccg agatttcagg tctatggtgg ggcgagaatg
      301 aacatggagt ggacgatggg agaatggaga tacctactac tggtgtaggt cgcgtccagt
      361 ttcgcattcg agcagtgatt gatcatctag ggatgcgagt ctttggagtc ttcctaattt
      421 tcttggacat catcctcatg atcattgatc tcagtcttat cgataaaagt gaatcttcac
      481 aatcctttta tgacgggatg gacttggctc tttcttgtta tttcatgctg gatttaggat
      541 taaggatatt tgcctacggg cccaagcttt tcttcaccaa cccttgggag gtggctgatg
      601 gtttgattat cgttgtcaca ttcgtcgtca cgatatttta cactgtgtta gatgaatacg
      661 gtaccgaaac aggagccgat ggtttggggc agttggttgt gttggccaga ttgctgcgtg
      721 tggttatctt agcaagaata ttttattcca ggcaacaagg ggatcccatg agtaaaggag
      781 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      841 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      901 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttct
      961 cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat gactttttca
     1021 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     1081 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     1141 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     1201 ataacgatca ccaggtgtac atcatggcag acaaacaaaa gaatggaatc aaagctaact
     1261 tcaaaattag acacaacatt gaagatggag gcgttcaact agcagaccat tatcaacaaa
     1321 atactccaat tggcgatggg cccgtccttt taccagacaa ccattacctg tttcacactt
     1381 ctactctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1441 cagctgatgg gattacacat ggcatggatg aactatacaa ataatctaga actcgagtct
     1501 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     1561 g
//
