LOCUS       sequence-266860-        8664 bp    DNA     circular SYN 14-APR-2025
DEFINITION  A shuttle vector for heterologous HA production with Corynebacterium
            glutamicum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Hoffmann J, Altenbuchner J
  TITLE     Hyaluronic acid production with Corynebacterium glutamicum: effect 
            of media composition on yield and molecular weight.
  JOURNAL   J Appl Microbiol. 2014 Sep;117(3):663-78. doi: 10.1111/jam.12553. 
            Epub 2014 Jun 20.
  PUBMED    24863652
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/jam.12553";
            journalName: "J Appl Microbiol"; date: "2014-09"; volume: "117"; 
            issue: "3"; pages: "663-78"
COMMENT     Sequence Label: pJOE7706.1
FEATURES             Location/Qualifiers
     source          1..8664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3072..3163)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(3182..3209)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3301..3387)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3599..3616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3600..3616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(3637..3708)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3915..4628)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVYGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(3960..3981)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4309..4328
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4568..4589
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(4635..4652)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4693..4715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4704..4720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4728..4734,4735..4750,4751..4756))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        4986..5063
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5064..6146
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5083..5102)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     primer_bind     complement(6161..6180)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6162..6180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6389..6580
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6574..6596)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6682..6824
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6737..6756
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6839..6856)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7010..7598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7090..7109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7706..8521)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7706..8521)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8429..8448
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8513..8532
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 cgcgtagatc tttgggagca gtccttgtgc gcttacgagg tgagccggtg gggaaccgtt
       61 atctgcctat ggtgtgagcc cccctagaga gcttcaagag caatcagccc gacctagaaa
      121 ggaggccaag agagagaccc ctacgggggg aaccgttttc tgcctacgag atggcacatt
      181 tactgggaag ctttacggcg tcctcgtgga agttcaatgc ccgcagactt aagtgctcta
      241 ttcacggtct gacgtgacac gctaaattca gacatagctt cattgattgt cggccacgag
      301 ccagtctctc cctcaacagt cataaaccaa cctgcaatgg tcaagcgatt tcctttagct
      361 ttcctagctt gtcgttgact ggacttagct agtttttctc gctgtgctcg ggcgtactca
      421 ctgtttgggt ctttccagcg ttctgcggcc tttttaccgc cacgtcttcc catagtggcc
      481 agagcttttc gccctcggct gctctgcgtc tctgtctgac gagcagggac gactggctgg
      541 cctttagcga cgtagccgcg cacacgtcgc gccatcgtct ggcggtcacg catcggcggc
      601 agatcaggct cacggccgtc tgctccgacc gcctgagcga cggtgtaggc acgctcgtag
      661 gcgtcgatga tcttggtgtc ttttaggcgc tcaccagccg cttttaactg gtatcccaca
      721 gtcaaagcgt ggcgaaaagc cgtctcatca cgggcggcac gccctggagc agtccagagg
      781 acacggacgc cgtcgatcag ctctccagac gcttcagcgg cgctcggcag gcttgcttca
      841 agcgtggcaa gtgcttttgc ttccgcagtg gcttttcttg ccgcttcgat acgtgcccgt
      901 ccgctagaaa actcctgctc atagcgtttt ttaggttttt ctgtgcctga gatcatgcga
      961 gcaacctcca taagatcagc taggcgatcc acgcgattgt gctgggcatg ccagcggtac
     1021 gcggtgggat cgtcggagac gtgcagtggc caccggctca gcctatgtga aaaagcctgg
     1081 tcagcgccga aaacgcgggt catttcctcg gtcgttgcag ccagcaggcg catattcggg
     1141 ctgctcatgc ctgctgcggc atacaccgga tcaatgagcc agatgagctg gcatttcccg
     1201 ctcagtggat tcacgccgat ccaagctggc gctttttcca ggcgtgccca gcgctccaaa
     1261 atcgcgtaga cctcggggtt tacgtgctcg attttcccgc cggcctggtg gctcggcaca
     1321 tcaatgtcca ggacaagcac ggctgcgtgc tgcgcgtgcg tcagagcaac atactggcac
     1381 cgggcaagcg attttgaacc aactcggtat aacttcggct gtgtttctcc cgtgtccggg
     1441 tctttgatcc aagcgctggc gaagtcgcgg gtcttgctgc cctggaaatt ttctctgccc
     1501 aggtgagcga ggaattcgcg gcggtcttcg ctcgtccagc cacgtgatcg cagcgcgagc
     1561 tcgggatggg tgtcgaacag atcagcggaa aatttccagg ccggtgtgtc aatgtctcgt
     1621 gaatccgcta gagtcatttt tgagcgcttt ctcccaggtt tggactgggg gttagccgac
     1681 gccctgtgag ttaccgctca cggggcgttc aacatttttc aggtattcgt gcagcttatc
     1741 gcttcttgcc gcctgtgcgc tttttcgacg cgcgacgctg ctgccgattc ggtgcaggtg
     1801 gtggcggcgc tgacacgtcc tgggcggcca cggccacacg aaacgcggca tttacgatgt
     1861 ttgtcatgcc tgcgggcacc gcgccacgat cgcggataat tctcgctgcc gcttccagct
     1921 ctgtgacgac catggccaaa atttcgctcg ggggacgcac ttccagcgcc atttgcgacc
     1981 tagccgcctc cagctcctcg gcgtggcgtt tgttggcgcg ctcgcggctg gctgcggcac
     2041 gacacgcatc tgagcaatat tttgcgcgcc gtcctcgcgg gtcaggccgg ggaggaatca
     2101 ggccaccgca gtaggcgcaa ctgattcgat cctccactac tgtgcgtcct cctggcgctg
     2161 ccgagcacgc agctcgtcag ccagctcctc aagatccgcc acgagagttt ctaggtcgct
     2221 cgcggcactg gcccagtctc gtgatgctgg cgcgtccgtc gtatcgagag ctcggaaaaa
     2281 tccgatcacc gtttttaaat cgacggcagc atcgagcgcg tcggactcca gcgcgacatc
     2341 agagagatcc atagctgatg attcgggcca attttggtac ttcgtcgtga aggtcatgac
     2401 accattataa cgaacgttcg ttaaagtttt tggcggaaaa tcacgcggca cgaaaatttt
     2461 cacgaagcgg gactttgcgc agctcagggg tgctaaaaat tttgtatcgc acttgatttt
     2521 tccgaaagac agattatctg caaacggtgt gtcgtatttc tggcttggtt tttaaaaaat
     2581 ctggaatcga aaatttgcgg ggcgaccgag aagtttttta caaaaggcaa aaactttttc
     2641 gggatcgaca gaaataaaac gatcgacggt acgcaacaaa aaagcgtcag gatcgccgta
     2701 gagcgattga agaccgtcaa ccaaagggga agcctccaat cgacgcgacg cgcgctctac
     2761 ggcgatcctg acgcagattt ttagctatct gtcgcagcgc cctcagggac aagccacccg
     2821 cacaacgtcg cgagggcgat cagcgacgcc gcagggggat caaaagtgct catcattgga
     2881 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2941 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3001 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3061 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3121 atgagcggat acatatttga atgtatttag aaaaataaac aaaagagttt gtagaaacgc
     3181 aaaaaggcca tccgtcagga tggccttctg cttaatttga tgcctggcag tttatggcgg
     3241 gcgtcctgcc cgccaccctc cgggccgttg cttcgcaacg ttcaaatccg ctcccggcgg
     3301 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     3361 tttcgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcatggggag
     3421 accccacact accatcggcg ctacggcgtt tcacttctga gttcggcatg gggtcaggtg
     3481 ggaccaccgc gctactgccg ccaggcaaat tctgttttat cagaccgctt ctgcgttctg
     3541 atttaatctg tatcaggctg aaaatcttct ctcatccgcc aaaacagcca agcttggctg
     3601 taaaacgacg gccagtgaat tcgagctcgg taccaggaga gcgttcaccg acaaacaaca
     3661 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt
     3721 tccctactct cgcatgggga gaccccacac taccatcggc gctacggcgt ttcacttctg
     3781 agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa ttctgtttta
     3841 tcagaccgct tctgcgttct gatttaatct gtatcaggct gaaaatcttc tctcatccgc
     3901 caaaacagcc aagcttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
     3961 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
     4021 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     4081 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
     4141 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
     4201 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     4261 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     4321 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     4381 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
     4441 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
     4501 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cgtacacgct gaacttgtgg
     4561 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     4621 cccttgctgg atccatgatg atgatgatga tgcgtaatca tggtcatatg tatatctcct
     4681 tcaattctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat tatacgagcc
     4741 gatgattaat tgtcaacagc tcatttcaga atatttgcca gaaccgttat gatgtcggcg
     4801 caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg cagtgattta
     4861 cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag cattggtgca
     4921 ccgtgcagtc gataagcccg gatcagcttg caattcgcgc gcgaaggcga agcggcattt
     4981 acgttgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag
     5041 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg
     5101 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
     5161 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg
     5221 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc
     5281 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca
     5341 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca
     5401 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg
     5461 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc
     5521 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc
     5581 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct
     5641 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga
     5701 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc
     5761 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg
     5821 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat
     5881 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt
     5941 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     6001 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca
     6061 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6121 tttcccgact ggaaagcggg cagtgagcgc aacgcaattg ccctatagtg agtcgtatta
     6181 atctagagtc gacctgcagg catgctggca ttgaccctga gtgatttttc tctggtcccg
     6241 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc
     6301 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa
     6361 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat
     6421 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
     6481 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
     6541 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     6601 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     6661 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     6721 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt
     6781 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     6841 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6901 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6961 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7021 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7081 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7141 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7201 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7261 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7321 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7381 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7441 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7501 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7561 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7621 cggggtttaa acgtgcaaca aagccgccgt cccgtcaagt cagcgtaatg ctctgccagt
     7681 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     7741 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     7801 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     7861 cgactcgccc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     7921 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaatagc ttatgcattt
     7981 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     8041 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     8101 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     8161 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga
     8221 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     8281 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     8341 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     8401 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     8461 catccatgtt ggaatttaat cgcggcctgg agcaagacgt ttcccgttga atatggctca
     8521 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
     8581 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa
     8641 atcgaacttt tgctgagttg aagg
//
