LOCUS       sequence-266874-       10458 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Agrobacterium T-DNA overexpression cassette comprising constitutive 
            TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene
            in F. solani genome. Replace eYFP w/ XhoI BamHI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Nielsen MR, Holzwarth AKR, Brew E, Chrapkova N, Kaniki SEK, 
            Kastaniegaard K, Sorensen T, Westphal KR, Wimmer R, Sondergaard TE, 
            Sorensen JL
  TITLE     A new vector system for targeted integration and overexpression of 
            genes in the crop pathogen Fusarium solani.
  JOURNAL   Fungal Biol Biotechnol. 2019 Dec 11;6:25. doi: 
            10.1186/s40694-019-0089-2. eCollection 2019.
  PUBMED    31890232
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2019-12-11"; volume: "6:25. doi"; pages: " 
            10.1186/s40694-019-0089-2. eCollection 2019"
COMMENT     Sequence Label: pSHUT4-eYFP
FEATURES             Location/Qualifiers
     source          1..10458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        517..732
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             733..1536
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             1850..2644
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             2943..4091
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4217..4241
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(5307..5559)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(5571..6284,6285..6287))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5616..5637)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5965..5984
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6224..6245
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             7422..8216
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVGDRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7476..7495)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8086..8105
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9332..9356
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9495..10126
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 catatatatt tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa
       61 gtaaaatgta aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa
      121 ttcggcatta atacctgaga gcaggaagag caagataaaa ggtagtattt gttggcgatc
      181 cccctagagt cttttacatc ttcggaaaac aaaaactatt ttttctttaa tttctttttt
      241 tactttctat ttttaattta tatatttata ttaaaaaatt taaattataa ttatttttat
      301 agcacgtgat gaattcgtaa tcatgagcaa aaggccagga tcacactgcc tttgctgagc
      361 tggatcaata gagtaacaaa agagtggtaa ggcctcgtta aaggacaagg acctgagcgg
      421 aagtgtatcg tacagtagac ggagtatact aggtatagtc tatagtccgt ggaattaatt
      481 ctcatgtttg acagcttatc atcgataagc tagcttttca attcatcatt ttttttttat
      541 tctttttttt gatttcggtt tccttgaaat ttttttgatt cggtaatctc cgaacagaag
      601 gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa
      661 gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa
      721 cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct
      781 gttgctgcca agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg
      841 gatgttcgta ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt
      901 ttactaaaaa cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg
      961 ctaaaggcat tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac
     1021 attggtaata cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca
     1081 gacattacga atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg
     1141 gcagaagaag taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag
     1201 ggctccctat ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa
     1261 gattttgtta tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat
     1321 tggttgatta tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag
     1381 tatagaaccg tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga
     1441 ctatttgcaa agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg
     1501 gaagcatatt tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca
     1561 tgtatactaa actcacaaat tagagcttca atttaattat atcagttatt acccattgaa
     1621 aaaggaagag tatgagtatt caacatttcc gtgtcgccca taattgtggt ttcaaaatcg
     1681 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
     1741 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
     1801 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
     1861 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
     1921 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
     1981 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
     2041 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
     2101 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
     2161 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
     2221 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
     2281 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
     2341 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
     2401 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
     2461 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
     2521 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     2581 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     2641 ttagtaccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc gacttcttcc
     2701 gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc aaggggtcgc
     2761 tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct
     2821 acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt
     2881 caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt
     2941 gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt
     3001 ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct
     3061 tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc
     3121 aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtctcg
     3181 aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga
     3241 ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg
     3301 ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata
     3361 ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt
     3421 tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg
     3481 tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac
     3541 tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct
     3601 tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga
     3661 aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg
     3721 ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga
     3781 aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc
     3841 actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt
     3901 tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca
     3961 tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg
     4021 aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt
     4081 gcaaacgcta gggccttgtg gggtcagttc cggctggatc tgctctcccg ctgacgccgt
     4141 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
     4201 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
     4261 ttaataacac attgcggacg tttttaatgt actggggtgg tttttctttt caccagtgag
     4321 acgggcaaca gcggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     4381 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     4441 gttgggtaac gccagggttt tcccagtcac gacgttgatg ccacgaattc caatctacgc
     4501 cgcattcagc agctcttcag ctcgaatctc gtgcgccaat cgcaccccgg aaaactctaa
     4561 aatagtccgc cgagagtgtg agtgtgttgt ggacgatcac gacgcgacgc gtcaacctcg
     4621 gtgtcggtct acggacggat gtttttgttg ttgcatttgt ggccattggt tggcaatgca
     4681 aaccatcttg tgcggaccga gctatcatgt tgcttgtctt gtcttgcgta caggttctgc
     4741 acaacattgc ataggagatc cgctcgatat ccttgtgcat tacgggagat cgatgcaagg
     4801 actttctgca cgccatcacg tacattacgt gccgggaaga ataacttctt ctttattcct
     4861 ttgttgtgtt tttgggagat tgcggctctt gaggctccat caagatttga ttttgttgtt
     4921 ccgcattccc cccaccacac aaaacaccac ccctaagggg ccgagaccac ggacgttttt
     4981 gtctcacttg gtgaagcacg actgccctgg tctgccatcc atcatctgga tgccggcact
     5041 cgtggccgcc cgagctacac acgcagacac ggcgccgtcg caagcgatgg tcgatttaac
     5101 gtcatgtcca tctgagctcg gcgctgcctc tccacttggt gtaggatagc tccaaaccgc
     5161 cgacgaagcg tggaccgaga acggagaagc ccatgtcagt atggagctct tttttctgtt
     5221 tagatctcgc tttgaatttc atgtggacct tttcaggacg aaattaatac actgacagag
     5281 acgagtatta agtcctcagc gggcccgatc tagtaacata gatgacaccg cgcgcgataa
     5341 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     5401 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     5461 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
     5521 caatcttaag aaactttatt gccaaatgtt tgaacgatcg gctcgagtta ttacttgtac
     5581 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     5641 tcgcgcttct cgttggggtc tttgctcagg gcggactggt agctcaggta gtggttgtcg
     5701 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     5761 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     5821 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     5881 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     5941 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     6001 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     6061 tcggggtagc gggcgaagca ctgcaggccg tagccgaagg tggtcacgag ggtgggccag
     6121 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     6181 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     6241 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgcccatgga tcctttgaag
     6301 gtggtgcgaa ctttgtagtt ctttgtaaga tactagaaag attctgtaga gaagcttgct
     6361 cagatactac ggctgatcgc gtagaggtac tgagcaaaac agatgtcagt aaggagaaga
     6421 gttgaatgaa tggaagaaga gtaggaaagg aggtatgggg gaaagatata cgtactgatg
     6481 cggacgaaga gagaaagaag gaaaaaagtt gtgggagggg aaggaggggg aatccttata
     6541 tggaggggca agcgagaagg cgaattagtg ggcgggctta agccctcgac cgccgccctt
     6601 atcattggac atggaggggt aatgccccca ccacgcatgt gcgggaccga cgcagaatct
     6661 gcacggcgga gtctcttcca gactgttgac ttttgggcga tgactcttgt tgctgcggcc
     6721 ttttgggtac accaacctcg ttgatcttgt ttccttggtt ctctttcgct cggagacccg
     6781 accatgaccc caccatcagt cactatcctg cctcgtcgat aaaaattttt tcttccctct
     6841 gattgttaca tagtatgttt ccacctttcc ggtggatttc ggacagtcaa actgggcatc
     6901 aacgcagtgg tgggctgctt cgtttgctgc gtgttgtact tgtttgcatt tgaaccccgc
     6961 ggtcgttcga gtccttaatt ggtccgctcc cggtcaacac ccaagcagct gtggcccggc
     7021 cgagtggcgc ctgtctggtc cacagtgaaa atcaccggtt cgaatcgtgg ctacccttgg
     7081 ccgaggttgc gatttctctg ccgtatctga caatgatcca accggccgga tgtgtgggag
     7141 ggcgcaaagg gtgtaattgg gtctaggcgt gagctgcatg tccgatggca gaaaaagaac
     7201 tactaaaact ctgtgacgtc ctcagggctg gtagctattt agcttgcccc tccctcaccc
     7261 cacctagctc aaacccaaca aaacacagca ccgaccacaa aaatctgcct cttaccacct
     7321 gctcatcacc ttttctcaca tataaagcag tgagctcagc ccgatttcca ttcctcaatt
     7381 caagtctatt aactctctca aagaggaacc caatcttcaa aatgattgaa caagatggat
     7441 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7501 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7561 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     7621 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     7681 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     7741 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     7801 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     7861 ggatggaagc cggtcttgtc gatcaggatg atctggacga agaacatcag gggctcgcgc
     7921 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     7981 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     8041 tcgactgtgg ccggctgggt gtgggggacc gctatcagga catagcgttg gctacccgtg
     8101 atattgctga agaacttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     8161 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaatca
     8221 ttccactcaa cattcaggct cctctgcgca cgtaaagtgc caaaggcaat accctgctcg
     8281 gtggaatgcc gccgggcttg tcgattttac gcacatatgc gcattcttga cttgaagcgg
     8341 aggagttctt cgttgcgggt tacagtgttt taataaaaga atggtcaaat caaactgcta
     8401 gatatacctg tcagacactc tagttgttga cccctatact cttaatacat cagacagtac
     8461 atgcatgttg catgactagt gctgaggcat taataacgac tttggcccca tgagcctcga
     8521 aataggaccc tagccagcca ccgcggagag ggatttgttt gccgatcggc ctggcctggt
     8581 ggtcaaaccc agccgtgtgg aatcgacaag catcccatgg cacttgggtt taggcccatc
     8641 ataagacaag cggttcaggc attgcgtcag cctggatcgt gagcactaga tgagcacttg
     8701 gcacgtctat cggttctcct agccgccgtt gaggaggcct tgtggaaatt ggactggtcg
     8761 gcgtcaacaa tattcagtaa aagccgagct tacgatagag tcctcggtgc atggccagat
     8821 caagacatga tcggtgggtt tcggatgact ccagtggcaa gatgactggt tcgcatgctg
     8881 atgctggggc tgaattgttt tttcgtctcc tctcctccct gggatatatt catatgcgtc
     8941 tacgatgcat cagactctgc atggaccaag ggatgaccca gcacagacgt cccatggatg
     9001 gagaagaagg atttctcatg gctgccttgt ttcaggtacg agtggtgttt tcgacgacat
     9061 gtttccatgg cttccgcagg tacacacatg tactgtaccg ttggacacca gttcgtgagc
     9121 actaggcttc gaacatggac agagtttgtg agccagcctt atcttggcct cgacctgact
     9181 ctactgcaac cgggatgtgg gttccctgct cccgtccttg gtcttcggcg ggcctctgcc
     9241 tcaacacgag gaggatcgcc tgtggcttat ggacttggtg cgctcatgat cagattgtcg
     9301 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     9361 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     9421 ttcgtccatt tgtttgttca tgccaaccac agggttccag atccgacgag caaggcaaga
     9481 ccgagcgcct ttccgacgct caccgggctg gttgccctcg ccgctgggct ggcggccgtc
     9541 tatggccctg caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc
     9601 cggcgttgtg gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt
     9661 gacacttgag gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt
     9721 cggccggcga cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg
     9781 agtttcccac agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg
     9841 aggggcgcga ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct
     9901 gacagatgag gggcgcacct attgacattt gaggggctgt ccacaggctg aaaatccagc
     9961 atttgcaagg gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt
    10021 aaaccaatat ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc
    10081 acgccgaagg ggggtgcccc cccttctcga accctcccgg cccgctaacg cgggcctccc
    10141 atccccccag ggtacgcatt gcgaataccg cttccacaaa cattgctcaa aagtatctct
    10201 ttgctatata tctctgtgct atatccctat ataacctacc catccacctt tcgctccttg
    10261 aacttggctc gaattcgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg
    10321 ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata
    10381 aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc
    10441 aacaaaaagc gtacttta
//
