LOCUS       sequence-266967-       10233 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Positive control when used in combination with pBWH-p53. Negative 
            control when used in combination with pBWH-Lam. Expression of 
            Gal4AD-largeT hybrid protein. Homology regions for recombination 
            with pB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, 
            Beltran-Sastre V, Serrano L, Maurer SP
  TITLE     rec-YnH enables simultaneous many-by-many detection of direct 
            protein-protein and protein-RNA interactions.
  JOURNAL   Nat Commun. 2018 Sep 14;9(1):3747. doi: 10.1038/s41467-018-06128-x.
  PUBMED    30217970
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-09-14"; volume: "9(1):3747. doi"; pages: " 
            10.1038/s41467-018-06128-x"
COMMENT     Sequence Label: pAWH-largeT
FEATURES             Location/Qualifiers
     source          1..10233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1475
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1557..1898
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1904..1923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1904..1922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1929..1938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1941..1967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2025..2049
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2169..2189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3943..4064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3980..3999)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4034..4053
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(4071..4095)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4543..4730
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4598..4622)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4847..5941)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5942..6347)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(6389..6405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6389..6405)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6402..6424)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6413..6429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6437..6443,6444..6461,6462..6467))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6482..6503
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6558..6591
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6669..6702
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6889..6906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7060..7648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7140..7159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7819..8610,8611..8679))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8442..8461
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8680..8784)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8852..8870
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8908..8930)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9030..9049
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      9066..10230
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg
     1981 aggccagtga attccacccg ggtgggcatc gatacgggat ctccacaagt ttgtacaaaa
     2041 aagcaggctt aggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     2101 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
     2161 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
     2221 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     2281 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
     2341 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
     2401 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
     2461 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat ccattttctg
     2521 ttattgagga aagtttgcca ggtgggttaa aggagcatga ttttaatcca gaagaagcag
     2581 aggaaactaa acaagtgtcc tggaagcttg taacagagta tgcaatggaa acaaaatgtg
     2641 atgatgtgtt gttattgctt gggatgtact tggaatttca gtacagtttt gaaatgtgtt
     2701 taaaatgtat taaaaaagaa cagcccagcc actataagta ccatgaaaag cattatgcaa
     2761 atgctgctat atttgctgac agcaaaaacc aaaaaaccat atgccaacag gctgttgata
     2821 ctgttttagc taaaaagcgg gttgatagcc tacaattaac tagagaacaa atgttaacaa
     2881 acagatttaa tgatcttttg gataggatgg atataatgtt tggttctaca ggctctgctg
     2941 acatagaaga atggatggct ggagttgctt ggctacactg tttgttgccc aaaatggatt
     3001 cagtggtgta tgacttttta aaatgcatgg tgtacaacat tcctaaaaaa agatactggc
     3061 tgtttaaagg accaattgat agtggtaaaa ctacattagc agctgctttg cttgaattat
     3121 gtggggggaa agctttaaat gttaatttgc ccttggacag gctgaacttt gagctaggag
     3181 tagctattga ccagttttta gtagtttttg aggatgtaaa gggcactgga ggggagtcca
     3241 gagatttgcc ttcaggtcag ggaattaata acctggacaa tttaagggat tatttggatg
     3301 gcagtgttaa ggtaaactta gaaaagaaac acctaaataa aagaactcaa atatttcccc
     3361 ctggaatagt caccatgaat gagtacagtg tgcctaaaac actgcaggcc agatttgtaa
     3421 aacaaataga ttttaggccc aaagattatt taaagcattg cctggaacgc agtgagtttt
     3481 tgttagaaaa gagaataatt caaagtggca ttgctttgct tcttatgtta atttggtaca
     3541 gacctgtggc tgagtttgct caaagtattc agagcagaat tgtggagtgg aaagagagat
     3601 tggacaaaga gtttagtttg tcagtgtatc aaaaaatgaa gtttaatgtg gctatgggaa
     3661 ttggagtttt agattggcta agaaacagtg atgatgatga tgaagacagc caggaaaatg
     3721 ctgataaaaa tgaagatggt ggggagaaga acatggaaga ctcagggcat gaaacaggca
     3781 ttgattcaca gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc
     3841 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     3901 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     3961 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4021 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatga acccagcttt
     4081 cttgtacaaa gtggtaagta actaagatcc atcgagctcg agctgcagat gaatcgtaga
     4141 tactgaaaaa ccccgcaagt tcacttcaac tgtgcatcgt gcaccatctc aatttctttc
     4201 atttatacat cgttttgcct tcttttatgt aactatactc ctctaagttt caatcttggc
     4261 catgtaacct ctgataacag ggtaattggc aaacagctat tatgggtatt atgggtgttt
     4321 aaactaggga taacagggta atacattcaa gcgcattagt aatgagtgaa tataactata
     4381 acggtcctaa cgagggctta ttcagaagct ttggacttct tcgccagagg tttggtcaag
     4441 tctccaatca aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag
     4501 ggtcaaatcg ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt
     4561 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
     4621 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg
     4681 ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg ccggtcgaaa
     4741 ttcccctacc ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt
     4801 catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt
     4861 tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat
     4921 caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt
     4981 cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag
     5041 ggttgacctt attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa
     5101 atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac
     5161 ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa
     5221 taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat
     5281 gttgaacctt caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata
     5341 atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat
     5401 gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt
     5461 gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac
     5521 ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg
     5581 gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt
     5641 gaagttcttt acggattttt agtaaacctt gttcaggtct aacactacct gtaccccatt
     5701 taggaccacc cacagcacct aacaaaacgg catcagcctt cttggaggct tccagcgcct
     5761 catctggaag tgggacacct gtagcttcga tagcagcacc accaattaaa tgattttcga
     5821 aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg
     5881 ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca
     5941 ttagaatggt atatccttga aatatatata tatattgctg aaatgtaaaa ggtaagaaaa
     6001 gttagaaagt aagacgattg ctaaccacct attggaaaaa acaataggtc cttaaataat
     6061 attgtcaact tcaagtattg tgatgcaagc atttagtcat gaacgcttct ctattctata
     6121 tgaaaagccg gttccggcgc tctcaccttt cctttttctc ccaatttttc agttgaaaaa
     6181 ggtatatgcg tcaggcgacc tctgaaatta acaaaaaatt tccagtcatc gaatttgatt
     6241 ctgtgcgata gcgcccctgt gtgttctcgt tatgttgagg aaaaaaataa tggttgctaa
     6301 gagattcgaa ctcttgcatc ttacgatacc tgagtattcc cacagttggg ggatctcgac
     6361 tctagctaga ggatcaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
     6421 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6481 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6541 acctgtcgtg ccagctgata acttcgtata atgtatgcta tacgaagtta ttaggtctga
     6601 agaggagttt acgtccagcc aagctagctt ggctgcaggt cgagcggccg cgatccggaa
     6661 cccttaatat aacttcgtat aatgtatgct atacgaagtt atcagctgca ttaatgaatc
     6721 ggccaacgcg ataacagggt aatacattca agcgcattag taatgagtga atataactat
     6781 aacggtccta aggtagcgaa aggaatcacc agttacatat atggtgatag taactataac
     6841 ggtcctaacg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7621 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7741 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7801 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7861 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7921 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7981 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8041 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8101 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8161 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8221 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8281 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8341 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8401 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8461 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8521 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8581 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8641 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8701 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8761 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     8821 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     8881 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8941 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     9001 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     9061 ccataacgca tttaagcata aacacgcact atgccgttct tctcatgtat atatatatac
     9121 aggcaacacg cagatatagg tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt
     9181 gcattttcgg aagcgctcgt tttcggaaac gctttgaagt tcctattccg aagttcctat
     9241 tctctagcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
     9301 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
     9361 taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc
     9421 cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta
     9481 cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat
     9541 tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga
     9601 gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca
     9661 ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt
     9721 tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc
     9781 aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga
     9841 cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag
     9901 taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa
     9961 aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa
    10021 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta
    10081 caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc
    10141 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg
    10201 ttctacaaaa tgaagcacag atgcttcgtt gct
//
