LOCUS       sequence-267015-        3273 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Cyan fluorescent tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Oehlers SH, Cronan MR, Scott NR, Thomas MI, Okuda KS, Walton EM, 
            Beerman RW, Crosier PS, Tobin DM
  TITLE     Interception of host angiogenic signalling limits mycobacterial 
            growth.
  JOURNAL   Nature. 2015 Jan 29;517(7536):612-5. doi: 10.1038/nature13967. Epub 
            2014 Nov 24.
  PUBMED    25470057
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13967"; journalName: "Nature"; date: "2015-01-29- 
            29"; volume: "517"; issue: "7536"; pages: "612-5"
COMMENT     Sequence Label: pME turquoise2
FEATURES             Location/Qualifiers
     source          1..3273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     153..172
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     412..433
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(484..583)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(599..616)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(599..615)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(608..630)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      679..765
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      857..884
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1043..1060)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1214..1802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1294..1313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1949..2758)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2672..2691
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2750..2769
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2871..2887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2871..2887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2892..2911
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2892..2910
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2928..3027
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(join(3032..3273,1..472,473..475,476..478))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3077..3098)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcttctgctt gtcggcggtg atatagacgt tgtcgctaaa gtagttgtac tccagcttgt
       61 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      121 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      181 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      241 tcatgtggtc ggggtagcgg gcgaagcact gcacgcccca ggacagggtg gtcacgaggg
      301 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
      361 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
      421 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
      481 tccagcctgc ttttttgtac aaagttggca ttataaaaaa gcattgctca tcaatttgtt
      541 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttggggcccg agcttaagac
      601 tggccgtcgt tttacaacgt cgtgactggg aaaacatcca tgctagcgtt aacgcgagag
      661 tagggaactg ccaggcatca aataaaacga aaggctcagt cggaagactg ggcctttcgt
      721 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
      781 ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
      841 aggcatcaaa ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
      901 cttcctggct agcggtacgc gtattaattg cgttgcgctc actgcccgct ttccagtcgg
      961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1621 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgtaactca
     1861 cgttaaggga ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta atgctctgcc
     1921 agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact
     1981 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     2041 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     2101 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     2161 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca
     2221 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     2281 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     2341 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     2401 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gtttttccgg
     2461 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     2521 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     2581 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaagc
     2641 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     2701 cagcatccat gttggaattt aatcgcggcc tcgacgtttc ccgttgaata tggctcataa
     2761 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     2821 tatcttgtgc aatgtaacat cagagatttt gagacacggg ccagagctgc caggaaacag
     2881 ctatgaccat gtaatacgac tcactatagg ggatatcagc tggatggcaa ataatgattt
     2941 tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc tttcttataa
     3001 tgccaacttt gtacaagaaa gctgggtaga tctacttgta gagctcgtcc atgccgagag
     3061 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     3121 ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     3181 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt
     3241 tgtggcggat cttgaagttg gccttgatgc cgt
//
