LOCUS       sequence-267044-        9270 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Neutrophil specific label.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Oehlers SH, Cronan MR, Scott NR, Thomas MI, Okuda KS, Walton EM, 
            Beerman RW, Crosier PS, Tobin DM
  TITLE     Interception of host angiogenic signalling limits mycobacterial 
            growth.
  JOURNAL   Nature. 2015 Jan 29;517(7536):612-5. doi: 10.1038/nature13967. Epub 
            2014 Nov 24.
  PUBMED    25470057
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13967"; journalName: "Nature"; date: "2015-01-29- 
            29"; volume: "517"; issue: "7536"; pages: "612-5"
COMMENT     Sequence Label: p5E LyzC
FEATURES             Location/Qualifiers
     source          1..9270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6476..6492
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6477..6493
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6527..6543)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6527..6543)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(6580..6598)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    6599..6722
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6795..6813)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6818..6834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6818..6834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6936..6955)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6947..7756
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7014..7033)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7903..8491
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8392..8411
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8645..8662
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8821..8848)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(8940..9026)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     9075..9097
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9089..9106
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9090..9106
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9146..9241
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9243..9261
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtaccctgat cactggtgta gtgaactcat caagggttaa tgagcagttg cagactgaac
       61 tgtgaaggtg gagttggttt atctctgttt gcaaagttat ttcattttac ttggttataa
      121 acagcaggca tgtgcagaag gggggtttgg gtgcttgagt acctgctctt tttttctctc
      181 gaagagaaag taccccataa aataaaaagt gccccctcgt ttgtgcacgg atcccctata
      241 cgcaacacca tccaacgctc tttagcgtcg cgattaaccc gtgccctaac cgttaaccaa
      301 attggttaac aagcaccagt tttgccttca attttttttt ctaaatgagc aactaacttt
      361 ctgtggtaaa gtaattgccc tgtgtgccat actgctgcta aggagtagat gatgtctgca
      421 gcgcagaatg atgacgcatg tcactcaaag attatttaaa agtcacatga aatccgacat
      481 aaccgttcac actgcggtca cattgcaaaa cattagatct tcacattaat gaaaaatttg
      541 acatcctggt tctatattgt atttgcaaga agttattcaa ttcaagattt ccaaatgtac
      601 aaaaatgaaa tgtgaaaatg tgtgaatatg ctgtctttca ctaataatgg catcctttaa
      661 aaataagagc aaggaaaata tctatattta ttgtaaacct tttcatcttc tgcatttgat
      721 gaattgttga acatctgagc agtgtgaaat cttctaacaa atggcctgat gtaatctgac
      781 catattttta acttaaaaca tctttttagc cttgagttta ctatgttggg cagtttgatc
      841 atcttttacc acaactttct tatctgaatc cattgccaac gttttgttgc cgatcatctg
      901 tgcagttcga attgacccta acgtgaccac caacaaggat tgaaggctca agtgtacccg
      961 tttttcttgt aataatcatc ttgcccacat tgtaaaaaat gctgggttcc acacaattcc
     1021 ttcatgttgt cccaacacaa gtcgattaaa ttaatttatt aaatggattg aacataaaac
     1081 aattcagtta tgccccccaa aaaatcaaga gctgtgttga tttagctcat tttaaactat
     1141 taggtttaac aagcagcaaa aaaaaaatat atatatatat atatatttca atacagacat
     1201 cagaaacaca tctcaatata gcagttcttc aaatttctgc ccttggtttg accacagact
     1261 gtatttgact cttttcttta ttttatagtc acttcacatg ttctaaactg ataataaaac
     1321 acaataatat tcagctgttc actaagtttt gacacatcat ctttctttca ttcctaaaaa
     1381 tgacctgtaa acactacaat gaacacgtct ttgctaaaac cgtatatgca gagcaaatga
     1441 attaaaaaaa aatgtaaagg tcctgtgaag tgctttgaaa tgtgcatttt ttatttgatg
     1501 tttgatgtaa tcttaaccaa aacacagaga gagggtggaa cattgcatag ctcctcccct
     1561 ttaaaaaaac agccagtagt gttttgtttt accacagtga gagtggttga gctcaagcgc
     1621 atcaaattaa aagcaaatga gaagcatctt gaagggggcg gggcttgtca gacactagag
     1681 agaatttgat tggttatgat gtaatgagaa actgaagtgt gacatgaata aaaccattga
     1741 tccatttagg tggaactgac aaactacaag ctttacatgt ttatgtgttt atcatcatct
     1801 aaacgcaaag tttatctctg ttttggagca cacaagctta tagatatctt aaaaacgaat
     1861 aatactgata ctaacatcta aaaacgttat tttattttca tgggaccttt aaatattctg
     1921 ccgttttatc ttcacacaag accgttttca tgccaacttt gctttgtctc agttcttctg
     1981 ctttggaaac aaaatttaac gctgctgcgt gtaaaagttt caattctttt cactcgtcac
     2041 agtacaaaga cacggaagtg tcgtgatgga agaaaaaaac tgggtgggaa aaaaatggaa
     2101 aacactcagt gataggaatt tttttttttt tttggttttt gcgtttttct cgcaaagata
     2161 tttgcatttc cttgcaaaat tgttgttcca caaacacttc ctgttcactt catacatgtc
     2221 aacccttccg tttttgccgg tgtggtattt taccactcta tcccgctatc atcctatcaa
     2281 taaacatttt cccatatttc tctcatattt taaatcttga aagcacattg aaattaatat
     2341 ataaatgttt gcattcgctt tatttccatt aaagctgttt gtcgatcatc agcctttata
     2401 tgcaacctct gaatgcatgt ataagcaagg gaacgcaaaa actttaaaat atttattttt
     2461 ctatcacttt tttttcttcc acccattttt ccttcaccat cacatccctt aggtctccgt
     2521 aacacagcat catgggctaa atttttttct tctgtgattc atatcgttct cagttgagtg
     2581 taaataatcc tgttgtaata actgatattt gcaggttcat atcaacacaa accactgagc
     2641 cgatttattg tttctcatat tttgctataa acccttcatc tactctatga aaaaaacaac
     2701 acaagaaatg attcatcgtt tttctggaat caatcataag tacagatcta aatggtttat
     2761 tgcttcatga gacaatatag ttttctccgt ggcaacctca gtgtagtagc acaacaaaca
     2821 agaggttgat catggggaaa atgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     2881 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     2941 gtgtgtgtgt gtgtgtgttg tgttagcttt ggtcagagga ggctgctgtg tttggccaga
     3001 gctctgctgg ccagggtcag aatcctgctt gttgaagagc ctcttcatca tgtggatcca
     3061 gaaactgaag atttgctgca acaggtgatc cacgcggagt ttaaagactg cacagtcctg
     3121 tttctcagtc aaaacccact cagtgtcctg cacacggaca ggtcagaaac cacactgaga
     3181 cacgtcctgt tattaatagt caagtttgag ggatttatga tgtggtgttg ttttcttcag
     3241 ggtgctggtt ttggacaagg gacaagctgt gaagtttgat gctccttcca ctctcctcca
     3301 gcaggggccg ctgttcagcc aataagatca gacctgctct tacactacag ttaatattga
     3361 caaatatttc agcgtatcca tttcacatca tattttagga acagtaaaac acagtattta
     3421 ttaatcagtt atgagtattt taagttaatt tgaatattaa tatcaatcag tttttagtta
     3481 atgaaggtta aattacaaca aacagatttt gaaatattct agcaagttgt gaggattact
     3541 ttcaattttt acatttcagc tgattttcgt tttgtttata tatatatata tattaaaata
     3601 tratkatytw crttttgttt atatatatat atatattaaa atataattat ctacattttg
     3661 atttgtagtg attaattttt attgatatta attttatatt attattagaa gtagtaaatt
     3721 aagttttaat tataacaaac aaattttaag tattgtaagt tgtgagggtt actttgactt
     3781 tgtttacatt cctttcagct actttacatt ttgttattta tttatttgta ttccttttat
     3841 atttatttgc taatataatt atgtagattt ttataatgtg ttattttaat tttttaaatt
     3901 tttttaatgt taaatgtata ttgtatattt agatttatta ttaagttgat tagtatgatg
     3961 tatgaattta ttactgaagg ttttttaaca attaattgaa gcaaaacaat atttaataag
     4021 cacagacata ccagtttaca gagtaactcc agtaaaggac tggtttattt atttcatttt
     4081 tcgccataca tttgattaga agagtttgac ttgggttgat tatagcaaat agtggaacac
     4141 aaacagattt tgaaatattc ttgcaaattt atttattaaa tccattcaaa tattgtcatt
     4201 ttttgtactg tgtaatttat attgatttgc tcatacaata tttacatttc gcttaaacaa
     4261 tacactatta tttatattta aaaatagcta tatataaatc aaatgtatag ctctttttta
     4321 cacaattaat caaagctaaa atatttgtca cacaaataca gtaagccact tcacaatgat
     4381 atcttcaaaa tctgtgcttg actgaatcat ttcatttatt gttttgtaat tcacattata
     4441 ccttacgttc tccaggcaca gtttgaattg ttgataaatg aagataatac atctgattta
     4501 attagtctcc tttgtttata ataactttaa tctaatttga tagtaataat tccagcaata
     4561 catccattac aataaaaatc gcttttttat tttgcacatt agtttgccgg tatttgttta
     4621 aagtttcacc atacttttaa atcatgtgct atttgaaaag ggctgttatg actggaatta
     4681 aatgtttttt tttatatcag tgttttagat tgaagtacaa tatttatagg taaaattcac
     4741 cagcatatgc ttaaagatag ccatgctcca ggtccttgtg tccttctaag gtctgtttgt
     4801 ggtttaagct gtttgttgac acacgcttgt cttctccatt tttctgtgaa gttattgtca
     4861 gtttattgca cactgggttg cattaagctg cagttttttc acaacagttt tccacattgt
     4921 aacagtcagt ctccaaatta ttccatcagt cctcaaattt agtcatttga ggccatgaaa
     4981 acacttaaaa ctacatgaag gtagtttggc attaggtttt agacttgttc ttgtttggca
     5041 tattgcaacc tgtgtaaaaa tgatttgatt tttatttttt tttttttttt tcagaaagaa
     5101 acggtccaaa aagagttatg tgaacattta tccatgtgtt taatgttttt taaattaaaa
     5161 aaaaacattt ttattaaacc acttgtgact aaaccttttt tagttattct attttgtgac
     5221 gagtctgaat ggccaaagta ctcttgaatc caaaatcttt atagtaaaat gtttaatctt
     5281 acttgcagtg ttgggtataa tttgtaacaa agttgaattc catatctgct gagtgaaaaa
     5341 gtaaagtaag agattgctgt aaatatttag gttatttatt tattaataca atatagaaag
     5401 taacaaaaca caatgtaaaa aatcaataaa gaaagtaaat attaaaagta atgataaact
     5461 aatgatgtaa acaatattag caacaaagtt tgtacaattc agggagggta aagaatttca
     5521 cctaatagtc aaactctgta ttaaataata taaaatcttt gttcttagtc tgaagaggag
     5581 actgtgagag aaagcttact ggttgttaaa ttagaaaatt tgtaacttgc agattaaaat
     5641 gatttaaata attttataaa ggccatgttt gaaacaaatg taaaaacata ataaatgtta
     5701 agaatttcag atattgagat tttacaattt aaaaaaagca ataaagtaat agcagagacc
     5761 tacactctta aagtaaatta cccaactctg cttactagta acttttggaa tagttactag
     5821 tatccattga acaaatacta gaatctaatt aaattgtgtg agtgtccaat gaaaagattc
     5881 ttgtatttaa aacagtacca gtatctatat atgtacaaaa tttagtgcta gcaaaatgca
     5941 aataaatact actgcatata atggattgca ttgtaaatcg actcgccata atacatttat
     6001 ttcctatttt tacttttaac aaaaaataag ttcactgagt atttcatgtc attaaatcgc
     6061 attaaacctc ttgcatgctt tatgtacact agtgcagttc gctaaatgac caccggtgtc
     6121 actaataaca cgatcgtttc tgaattctgc attaaacgcc atttatacgt tgctcttatt
     6181 agtaaacaat ttctccatca tgagctcgac cagtattgca tatttcatcc tcttaaatcc
     6241 agagttgttt cttttaatca ctttctcttc atgataaaag cagtttttgg ttttcagaag
     6301 tttacaacaa acccagtcag ttattgcctc agcaaacact ggagcaactg cacacttgtg
     6361 aaaagcttct tgcgaaacac aaacctttct ctggacttat aaataaaggc attcagcagc
     6421 tccaccatac acctccactg gtcttgataa agcagatatc agcagtgata caatctcgag
     6481 gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cactagttct
     6541 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattca
     6601 agtttgtaca aaaaagttga acgagaaacg taaaatgata taaatatcaa tatattaaat
     6661 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat gcagtcacta
     6721 tgaaccaact acttagatgg tattagtgac ctgtagaatt cgagctctag agctgcaggg
     6781 cggccgcgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     6841 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     6901 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     6961 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     7021 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     7081 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     7141 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     7201 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     7261 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     7321 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     7381 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     7441 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     7501 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     7561 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     7621 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     7681 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     7741 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     7801 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     7861 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7921 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7981 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8041 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8101 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8161 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8221 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8281 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8341 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8401 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8461 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8521 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8581 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     8641 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     8701 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     8761 cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca
     8821 aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg
     8881 cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga
     8941 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     9001 tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct
     9061 agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc
     9121 cgagttaacg ctaccatgga gctccaaata atgattttat tttgactgat agtgacctgt
     9181 tcgttgcaac aaattgataa gcaatgcttt tttataatgc caactttgta tagaaaagtt
     9241 ggtaatacga ctcactatag ggcgaattgg
//
