LOCUS       sequence-267108-        5817 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Part Plasmid for dCas9 NLS Stop, Part 3b(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM766
FEATURES             Location/Qualifiers
     source          1..5817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4168..4188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(4226..4260)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4331..4990)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4905..4924
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5172..5760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5661..5680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgttctttc ctgcgttatc ccctgattct gtggataacc gtagtcggtc tcattctgac
       61 aagaagtatt ctatcggact ggccatcggg actaatagcg tcgggtgggc cgtgatcact
      121 gacgagtaca aggtgccctc taagaagttc aaggtgctcg ggaacaccga ccggcattcc
      181 atcaagaaaa atctgatcgg agctctcctc tttgattcag gggaaaccgc tgaagcaacc
      241 cgcctcaagc ggactgctag acggcggtac accaggagga agaaccggat ttgttacctt
      301 caagagatat tctccaacga aatggcaaag gtcgacgaca gcttcttcca taggctggaa
      361 gaatcattcc tcgtggaaga ggataagaag catgaacggc atcccatctt cggtaatatc
      421 gtcgacgagg tggcctatca cgagaaatac ccaaccatct accatcttcg caaaaagctg
      481 gtggactcaa ccgacaaggc agacctccgg cttatctacc tggccctggc ccacatgatc
      541 aagttcagag gccacttcct gatcgagggc gacctcaatc ctgacaatag cgatgtggat
      601 aaactgttca tccagctggt gcagacttac aaccagctct ttgaagagaa ccccatcaat
      661 gcaagcggag tcgatgccaa ggccattctg tcagcccggc tgtcaaagag ccgcagactt
      721 gagaatctta tcgctcagct gccgggtgaa aagaaaaatg gactgttcgg gaacctgatt
      781 gctctttcac ttgggctgac tcccaatttc aagtctaatt tcgacctggc agaggatgcc
      841 aagctgcaac tgtccaagga cacctatgat gacgatctcg acaacctcct ggcccagatc
      901 ggtgaccaat acgccgacct tttccttgct gctaagaatc tttctgacgc catcctgctg
      961 tctgacattc tccgcgtgaa cactgaaatc accaaggccc ctctttcagc ttcaatgatt
     1021 aagcggtatg atgagcacca ccaggacctg accctgctta aggcactcgt ccggcagcag
     1081 cttccggaga agtacaagga aatcttcttt gaccagtcaa agaatggata cgccggctac
     1141 atcgacggag gtgcctccca agaggaattt tataagttta tcaaacctat ccttgagaag
     1201 atggacggca ccgaagagct cctcgtgaaa ctgaatcggg aggatctgct gcggaagcag
     1261 cgcactttcg acaatgggag cattccccac cagatccatc ttggggagct tcacgccatc
     1321 cttcggcgcc aagaggactt ctaccccttt cttaaggaca acagggagaa gattgagaaa
     1381 attctcactt tccgcatccc ctactacgtg ggacccctcg ccagaggaaa tagccggttt
     1441 gcttggatga ccagaaagtc agaagaaact atcactccct ggaacttcga agaggtggtg
     1501 gacaagggag ccagcgctca gtcattcatc gaacggatga ctaacttcga taagaacctc
     1561 cccaatgaga aggtcctgcc gaaacattcc ctgctctacg agtactttac cgtgtacaac
     1621 gagctgacca aggtgaaata tgtcaccgaa gggatgagga agcccgcatt cctgtcaggc
     1681 gaacaaaaga aggcaattgt ggaccttctg ttcaagacca atagaaaggt gaccgtgaag
     1741 cagctgaagg aggactattt caagaaaatt gaatgcttcg actctgtgga gattagcggg
     1801 gtcgaagatc ggttcaacgc aagcctgggt acctaccatg atctgcttaa gatcatcaag
     1861 gacaaggatt ttctggacaa tgaggagaaa gaggacatcc ttgaggacat tgtcctgact
     1921 ctcactctgt tcgaggaccg ggaaatgatc gaggagaggc ttaagaccta cgcccatctg
     1981 ttcgacgata aagtgatgaa gcaacttaaa cggagaagat ataccggatg gggacgcctt
     2041 agccgcaaac tcatcaacgg aatccgggac aaacagagcg gaaagaccat tcttgatttc
     2101 cttaagagcg acggattcgc taatcgcaac ttcatgcaac ttatccatga tgattccctg
     2161 acctttaagg aggacatcca gaaggcccaa gtgtctggac aaggtgactc actgcacgag
     2221 catatcgcaa atctggctgg ttcacccgct attaagaagg gtattctcca gaccgtgaaa
     2281 gtcgtggacg agctggtcaa ggtgatgggt cgccataaac cagagaacat tgtcatcgag
     2341 atggccaggg aaaaccagac tacccagaag ggacagaaga acagcaggga gcggatgaaa
     2401 agaattgagg aagggattaa ggagctcggg tcacagatcc ttaaagagca cccggtggaa
     2461 aacacccagc ttcagaatga gaagctctat ctgtactacc ttcaaaatgg acgcgatatg
     2521 tatgtggacc aagagcttga tatcaacagg ctctcagact acgacgtgga cgccatcgtc
     2581 cctcagagct tcctcaaaga cgactcaatt gacaataagg tgctgactcg ctcagacaag
     2641 aaccggggaa agtcagataa cgtgccctca gaggaagtcg tgaaaaagat gaagaactat
     2701 tggcgccagc ttctgaacgc aaagctgatc actcagcgga agttcgacaa tctcactaag
     2761 gctgagaggg gcggactgag cgaactggac aaagcaggat tcattaaacg gcaacttgtg
     2821 gagactcggc agattactaa acatgtagcc caaatccttg actcacgcat gaataccaag
     2881 tacgacgaaa acgacaaact tatccgcgag gtgaaggtga ttaccctgaa gtccaagctg
     2941 gtcagcgatt tcagaaagga ctttcaattc tacaaagtgc gggagatcaa taactatcat
     3001 catgctcatg acgcatatct gaatgccgtg gtgggaaccg ccctgatcaa gaagtaccca
     3061 aagctggaaa gcgagttcgt gtacggagac tacaaggtct acgacgtgcg caagatgatt
     3121 gccaaatctg agcaggagat cggaaaggcc accgcaaagt acttcttcta cagcaacatc
     3181 atgaatttct tcaagaccga aatcaccctt gcaaacggtg agatccggaa gaggccgctc
     3241 atcgagacta atggggagac tggcgaaatc gtgtgggaca agggcagaga tttcgctacc
     3301 gtgcgcaaag tgctttctat gcctcaagtg aacatcgtga agaaaaccga ggtgcaaacc
     3361 ggaggctttt ctaaggaatc aatcctcccc aagcgcaact ccgacaagct cattgcaagg
     3421 aagaaggatt gggaccctaa gaagtacggc ggattcgatt caccaactgt ggcttattct
     3481 gtcctggtcg tggctaaggt ggaaaaagga aagtctaaga agctcaagag cgtgaaggaa
     3541 ctgctgggta tcaccattat ggagcgcagc tccttcgaga agaacccaat tgactttctc
     3601 gaagccaaag gttacaagga agtcaagaag gaccttatca tcaagctccc aaagtatagc
     3661 ctgttcgaac tggagaatgg gcggaagcgg atgctcgcct ccgctggcga acttcagaag
     3721 ggtaatgagc tggctctccc ctccaagtac gtgaatttcc tctaccttgc aagccattac
     3781 gagaagctga aggggagccc cgaggacaac gagcaaaagc aactgtttgt ggagcagcat
     3841 aagcattatc tggacgagat cattgagcag atttccgagt tttctaaacg cgtcattctc
     3901 gctgatgcca acctcgataa agtccttagc gcatacaata agcacagaga caaaccaatt
     3961 cgggagcagg ctgagaatat catccacctg ttcaccctca ccaatcttgg tgcccctgcc
     4021 gcattcaagt acttcgacac caccatcgac cggaaacgct atacctccac caaagaagtg
     4081 ctggacgcca ccctcatcca ccagagcatc accggacttt acgaaactcg gattgacctc
     4141 tcacagctcg gaggggatga gggagctccc aagaaaaagc gcaaggtagg tagttccgga
     4201 tcctagggat cctgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     4261 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     4321 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     4381 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     4441 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     4501 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga
     4561 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     4621 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     4681 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     4741 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     4801 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     4861 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     4921 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     4981 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     5041 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg
     5101 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5161 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5221 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5281 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5341 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5401 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5461 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5521 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5581 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5641 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5701 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5761 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcac
//
