LOCUS       sequence-267241-        6812 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses split GFP fragment (GFP1-10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Kondo S, Takahashi T, Yamagata N, Imanishi Y, Katow H, Hiramatsu S, 
            Lynn K, Abe A, Kumaraswamy A, Tanimoto H
  TITLE     Neurochemical Organization of the Drosophila Brain Visualized by 
            Endogenously Tagged Neurotransmitter Receptors.
  JOURNAL   Cell Rep. 2020 Jan 7;30(1):284-297.e5. doi: 
            10.1016/j.celrep.2019.12.018.
  PUBMED    31914394
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.12"; journalName: "Cell Rep"; date: 
            "2020-01-7- 7"; volume: "30"; issue: "1"; pages: "284-297.e5"
COMMENT     Sequence Label: pBFv-UAS3-GFP1-10
FEATURES             Location/Qualifiers
     source          1..6812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(179..296,373..466,558..1164,1219..1352,
                     1413..1573,1647..1672))
                     /codon_start=1
                     /product="tryptophan oxygenase"
                     /label=vermilion
                     /note="selectable marker, required to synthesize the brown 
                     eye pigment in Drosophila"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
     protein_bind    1944..2038
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    2070..2164
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        2193..2431
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2378..2399
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(3682..3702)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3682..3700)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3721..3737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3721..3737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3734..3756)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3745..3761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3769..3775,3776..3793,3794..3799))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3814..3835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3952..3969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4203..4222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4882..5673,5674..5742))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5505..5524
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5743..5847)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5873..6328)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6010..6031)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6222..6241
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6455..6477
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6469..6486
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6470..6486
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6496..6515
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6496..6514
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6722..6791
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 aagcttggat ttattttgtt atgttatatg tattatatgt cagacataaa gaaaaggaac
       61 acatcaaatg tgataacaaa gactaaacaa gtaattttat tacaccaaaa cgacaaaaca
      121 gtaggcagaa caaacaacgc atagccaaac attgacgaat tggataccct gccgattgtc
      181 agacactttt gttgatcagt ttcttgcgaa tggtctcgtc cagcggtgga atcgcctcgc
      241 ggggaatcag aaaagtggac agattgaaca gatccagaaa caccttgtac cgatcactga
      301 aaccaaaaaa aaacaaaggg agaacagttt gagttcattg atccccgata taatcacatc
      361 tgcgatgatc acctgagagt ggagcgcaga tattgatatc cagacgagcc accagtgccc
      421 aactgttggg atccaatcat gcgttgcacc atgatcacgt gattgtctgc ggcgggaata
      481 gaaagtattt ggttaggaaa accagtctta aacataagat atatttataa aagagtatca
      541 aagaatgcaa tacttacatc tccacttggt tattaacgag tcgatgtcca tgagcagggt
      601 gagcaactgg tgtggttggc tgaacctggg ttcatcccta tagaaggtga tcatgatggc
      661 tccctgaagg gcacgatggc taaaccggcg atccccacga cgcaccagtg catcgtgcac
      721 tgccggatca aagatggagc gatacacctc gcgtcgcttc tcaatgtcca tgaggcggta
      781 gtttttcgcc ttctccacgg gctcctccat ggcgctctgt acctgcgcct ccaggaatcg
      841 atcgacgctc tcctgaaact tggcccagaa gttgaagcca ctctcctcca gtccgggcgt
      901 cctctccagc catcgctgca ctagctccag tagcgaggga tctttctccg agttgcgaat
      961 cgagttccgc gcctcctcgt cgctaaagac atccgagtac ttctggttgt atctcacccg
     1021 ctgctctgtc agaactccca gcttgttctc gatcaaacgg aactgcagcg actgaaaacc
     1081 agatgcgggt gccaggtact tgcggaagtc catgaagtct agcggggtca tggtctccag
     1141 aatgggcact tggtccacca ggagctgtac aaaggaagtt ataaacggat tttggtaaga
     1201 gattcagaaa gcactcactt ttagaatcag aaccactcgg ttcagtcgct tgacaatctc
     1261 cagcgtcttg gtttcatcga tgacctctgc atccaacatg tctcgtatgg agtcgaactc
     1321 aaagatgatc tgcttgaacc aaagctcgta ggctgtggcg aaggtactta aatgccattg
     1381 agtgttgtca tcaaagttgt aaacctactc accctggtgc gtgatgatga acagatgctc
     1441 atcgtgcacg ggtcgcttgt cctcctcgga cagcatacac tgggcatcca gcagtttgtc
     1501 cagcatcaga tactctccat agattttgcc cacttccgtg gttaatggca ccgccgaatc
     1561 atcgtgatcg tttctgtatg ggtttgaatt gaatcgcaga actgaagatc gattggcatt
     1621 cctggacagc acgtgctggt gctcacccgt ttcctgcata gggacagctc atggtgcaca
     1681 gctcagatca gatcgtgact cctcgagcgg cggatgctgg cgaactgatc tccgccagcg
     1741 gaccggagat gagaccccag cgaaccgata acagagcgag agagctccag ttccgactga
     1801 ttgcacagtc ggtgatctgg gcgatgggca ctgccagata ggctgggaat tatcaatcac
     1861 ttgaggtgaa agtgcggcgc acacaaatcc aagcttgata tcgaattccc tgcagggcca
     1921 accaacaacc ttaagctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
     1981 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     2041 gagactctag cgagcacgcg tctgcaggtc ggagtactgt cctccgagcg gagtactgtc
     2101 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     2161 cgagcggaga ctctagcgag ccatatggag cgccggagta taaatagagg cgcttcgtct
     2221 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
     2281 aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga
     2341 atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga
     2401 agtaattatt gaatacaaga agagaactct gaatagggaa ttggggcgcg cccaaaatgt
     2461 ccaaaggaga agagctgttc actggagtgg taccaatact tgtggagttg gacggagatg
     2521 tgaacggaca caaattttca gtccgcgggg agggggaagg ggatgctact attggcaagc
     2581 tgacgctcaa attcatctgt accaccggaa aactccctgt accctggccc acactggtga
     2641 caactctgac ttacggcgtg caatgtttta gccgataccc agaccacatg aagaggcacg
     2701 actttttcaa aagcgcaatg cctgaaggat acgtacagga aaggaccatt tcttttaaag
     2761 acgacgggaa gtacaaaacc cgggcagtgg tgaagtttga gggcgatacc ctcgtcaata
     2821 ggatcgaatt gaagggaact gacttcaaag aagatggcaa catcctgggt cacaagcttg
     2881 agtataactt taactcccac aacgtgtata ttacagccga caaacagaag aatggaatta
     2941 aggctaactt cactgtcaga cacaatgtcg aagatggctc cgtgcagctc gccgatcact
     3001 atcaacagaa tactcctatc ggggacggcc cagtcctgct gcccgacaac cactacctga
     3061 gtacccagac tgttctgagc aaagatccga acgagaagta agcggccgcg cgtcacgcca
     3121 cttcaacgct cgatgggagc gtcattggtg ggcggggtaa ccgtcgaaat cagtgtttac
     3181 gcttccaatc gcaacaaaaa attcactgca acactgaaaa gcatacgaaa acgatgaaga
     3241 ttgtacgaga aaccataaag tattttatcc acaaagacac gtatagcaga aaagccaagt
     3301 taactcggcg ataagttgtg tacacaagaa taaaatcggc cagattcagt gttgtcagaa
     3361 ataagaaaac cccactatgt ttttctttgc cttttctttc tcccagcgat cattcatttc
     3421 gtggtgaaag aacggggtca ttgcacggag tttcgactgc gggaaagcag agctgccgtt
     3481 cacttcgtct ataattagcg ctttctattt tccccgattc gggccgctgc tgcgcttttc
     3541 cgcctgctgt ttgtggcaag tgtagcagca ggctgtgcac gcagtgtgta gggataacag
     3601 ggtaatgacg tcgcatgcac ttggctttcc accgttggta tcgattctct gggacgatga
     3661 gtcgagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     3721 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3781 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3841 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3901 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4801 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4861 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4921 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4981 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5041 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5101 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5161 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5221 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5281 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5341 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5401 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5461 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5521 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5581 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5641 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5701 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5761 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5821 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     5881 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     5941 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     6001 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     6061 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     6121 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     6181 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     6241 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     6301 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     6361 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     6421 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     6481 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctc gacatgcccg
     6541 ccgtgaccgt cgagaacccg ctgacgctgc cccgcgtatc cgcacccgcc gacgccgtcg
     6601 cacgtcccgt gctcaccgtg accaccgcgc ccagcggttt cgagggcgag ggcttcccgg
     6661 tgcgccgcgc gttcgccggg atcaactacc gccacctcga cccgttcatc atgatggacc
     6721 agatgggtga ggtggagtac gcgcccgggg agcccaaggg cacgccctgg cacccgcacc
     6781 gcggcttcga gaccgtgacc tacatcgtcg ac
//
