LOCUS       sequence-267251-        3793 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway compatible middle entry clone contains human full length 
            NFR2F2 gene with stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, 
            Lawson ND
  TITLE     Genomic Characterization of Endothelial Enhancers Reveals a 
            Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene 
            Expression.
  JOURNAL   Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075.
  PUBMED    32065070
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075."
COMMENT     Sequence Label: pME-NR2F2
FEATURES             Location/Qualifiers
     source          1..3793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      669..678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1552..1569
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     protein_bind    complement(1920..2019)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2036..2055)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2037..2055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2060..2076)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2060..2076)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2178..2197)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2189..2998
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2256..2275)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3145..3733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3634..3653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgc caccatggca atggtagtca gcacgtggcg cgacccccag gacgaggtgc
      721 ccggctcaca gggcagccag gcctcgcagg cgccgcccgt gcccggcccg ccgcccggcg
      781 ccccgcacac gccacagacg cccggccaag ggggcccagc cagcacgcca gcccagacgg
      841 cggccggtgg ccagggcggc cctggcggcc cgggtagcga caagcagcag cagcagcaac
      901 acatcgagtg cgtggtgtgc ggagacaagt cgagcggcaa gcactacggc cagttcacgt
      961 gcgagggctg caagagcttc ttcaagcgca gcgtgcggag gaacctgagc tacacgtgcc
     1021 gcgccaaccg gaactgtccc atcgaccagc accatcgcaa ccagtgccag tactgccgcc
     1081 tcaaaaagtg cctcaaagtg ggcatgagac gggaagcggt gcagaggggc aggatgccgc
     1141 cgacccagcc gacccacggg cagttcgcgc tgaccaacgg ggatcccctc aactgccact
     1201 cgtacctgtc cggatatatt tccctgctgt tgcgcgcgga gccctatccc acgtcgcgct
     1261 tcggcagcca atgcatgcag cccaacaaca tcatgggtat cgagaacatt tgcgaactgg
     1321 ccgcgaggat gctcttcagc gccgtcgagt gggcccggaa catccccttc ttccccgacc
     1381 tgcagatcac ggaccaggtg gccctgcttc gcctcacctg gagcgagctg tttgtgttga
     1441 atgcggcgca gtgctccatg cccctccacg tcgccccgct cctggccgcc gccggcctgc
     1501 atgcttcgcc catgtccgcc gaccgggtgg tcgcctttat ggaccacata cggatcttcc
     1561 aagagcaagt ggagaagctc aaggcgctgc acgttgactc agccgagtac agctgcctca
     1621 aggccatagt cctgttcacc tcagatgcct gtggtctctc tgatgtagcc catgtggaaa
     1681 gcttgcagga aaagtctcag tgtgctttgg aagaatacgt taggagccag taccccaacc
     1741 agccgacgag attcggaaag cttttgcttc gcctcccttc cctccgcacc gtctcctcct
     1801 cagtcataga gcaattgttt ttcgtccgtt tggtaggtaa aacccccatc gaaaccctca
     1861 tccgggatat gttactgtcc ggcagcagtt ttaactggcc gtatatggca attcaataaa
     1921 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     1981 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     2041 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     2101 tctctgatgt tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc
     2161 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     2221 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     2281 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     2341 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     2401 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     2461 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     2521 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     2581 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     2641 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     2701 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     2761 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     2821 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     2881 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     2941 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     3001 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     3061 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     3121 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3181 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3241 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3301 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3361 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3421 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3481 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3541 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3601 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3661 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3721 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3781 ttttgctcac atg
//
