LOCUS       sequence-26750-e         775 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: mId1-Venus
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..262)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDEXYK"
     primer_bind     complement(53..74)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     402..421
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctcgagtcn cttgtacngc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
       61 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc
      121 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      181 agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct
      241 tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact
      301 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      361 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      421 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      481 cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag cccagggtgg
      541 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatcagc ttcagggtca
      601 gcttgccgta ngtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
      661 cgtcgccgtc cagctcgacn naggatgggc accaccccgg tgaacagctc ctcgcccttg
      721 ctcaccatac cagatcctnn ngctgatcca gcananngcg ncncanatgc natcg
//
