LOCUS       sequence-267521-        9568 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian expression of SPARK protease component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Sanchez MI, Ting AY
  TITLE     Directed evolution improves the catalytic efficiency of TEV 
            protease.
  JOURNAL   Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7.
  PUBMED    31819267
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7."
COMMENT     Sequence Label: CMV:eGFP-p2A-HA-beta-2Ar-uTEV1-Delta(220-242)
FEATURES             Location/Qualifiers
     source          1..9568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(230..232,233..235,236..946)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(230..232,233..235,236..946)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(275..296)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(536..555)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     883..904
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             956..1012
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1013..1039
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1046..1070
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2298..2322)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3041..3629
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3094..3114)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3512..3523)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3701..3934
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4012..4133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4049..4068)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4103..4122
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4173..4308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4328..4347)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4329..4347)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4357..4374)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4357..4373)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4366..4388)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4515..4970
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4602..4621)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4812..4833
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4996..5100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5101..5169,5170..5961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5319..5338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6132..6720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6621..6640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6874..6891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7008..7029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7044..7049,7050..7067,7068..7074)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7082..7098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7087..7109
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7106..7122
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7106..7122
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7141..7161
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7143..7161
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7189..7415
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7416..7596
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7643..7768
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8261..8494
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8679..8723
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9031..9148
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9265..9568
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactgtcg ggatccacca tggtgagcaa
      241 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      301 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      361 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      421 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      481 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      541 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      601 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      661 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      721 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      781 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      841 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      901 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggaa gcggagctac
      961 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac cttacccata
     1021 tgacgttcca gattacgcta ccggtccaac tttgtacaaa aaagttggca tgggtgaaaa
     1081 acccgggacc agggtcttca agaagtcgag ccctaactgc aagctcaccg tgtacttggg
     1141 caagcgtgac tttgtggatc acttggacaa agtggatcct gtcgatggtg tggtgcttgt
     1201 ggatcctgac tacttgaagg accggaaagt gtttgtgacc ctcacctgtg ccttccgcta
     1261 tggccgagaa gacctggatg tactgggcct gtctttccgc aaagatctgt tcatcgccac
     1321 ctaccaggcc ttccccccca tgcccaaccc acctcggccc cccacccgcc tacaggaccg
     1381 actgctgaag aagttgggcc agcacgccca cccctttttt ttcacaatac cccagaattt
     1441 gccttgctcc gtcacactgc agccaggacc ggaggacaca gggaaggcct gtggagtaga
     1501 ctttgagatt cgagccttct gtgccaaatc tatagaagaa aaaagccaca aaaggaactc
     1561 cgtgcggctt atcatcagaa aggtacagtt tgctcctgag acacccggcc cccagccatc
     1621 agctgaaacc acacgccact tcctcatgtc tgaccggagg tccctgcacc tagaggcttc
     1681 cctggacaaa gagctgtact accatgggga acccctcaat gtcaacgtcc acgtcaccaa
     1741 caattctgcc aaaaccgtca agaagatcag agtgtctgtg agacagtatg ccgacatttg
     1801 cctcttcagc accgcgcagt acaagtgtcc tgtggctcag cttgaacaag atgaccaggt
     1861 gtctcccagt tccacattct gcaaggtgta caccataacc ccgctgctca gtgacaaccg
     1921 agagaagcgt ggccttgccc ttgatgggca actcaagcac gaagacacca acctggcttc
     1981 cagcaccatt gtgaaggagg gagccaacaa ggaggtgctg ggaatcctag tatcctacag
     2041 ggtcaaggtg aagctggtgg tgtctcgagg cggggatgtc tccgtggagc tacctttcgt
     2101 cctaatgcac cccaagcccc acgaccacat cacccttccc cgaccccagt cagccccccg
     2161 ggaaatagac atccctgtgg ataccaacct cattgaattc gataccaact atgccacaga
     2221 cgacgacatc gtgtttgagg actttgcgag gcttcggctg aaggggatga aggatgacga
     2281 ctgtgatgac cagttctgcc caactttctt gtacaaagtt ggcattatag ctagcggtgg
     2341 aagtggatca ggcagcggtg gatctggaga aagcttgttt aagggaccac gtgattacaa
     2401 cccgatatcg agcaccattt gtcatttgac gaatgaatct gatgggcaca caacatcgtt
     2461 gtatggtatt ggatttggtc ccttcatcat tacaaacaag cacttgttta gaagaaataa
     2521 tggaacactg ttggtccaat cactacatgg tgtattcaag gtcaagaaca ccacgacttt
     2581 gcaacaacac ctcattgatg ggagggacat gataattatt cgcatgccta aggatttccc
     2641 accatttcct caaaagctga aatttagaga gccacaaagg gaagagcgca tatgtcttgt
     2701 gacaaccaac ttccaaacta agagcatgtc tagcatggtg tcagacacta gttgcacatt
     2761 cccttcatct gatggcatat tctggaagca ttggattcaa accaaggatg ggcagtgtgg
     2821 caatccatta gtatcaacta gagatgggtt cattgttggt atacactcag catcgaattt
     2881 caccaacaca aacaattatt tcacaagcgt gccgaaaaac ttcatggaat tgttgacaaa
     2941 tcaggaggcg cagcagtggg ttagtggttg gcgattaaat gctgactcag tattgtgggg
     3001 tggccataaa gttttcatgg tgtaaacgcg ttaagtcgac aatcaacctc tggattacaa
     3061 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3121 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3181 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3241 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3301 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3361 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3421 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3481 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3541 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3601 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     3661 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     3721 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     3781 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3841 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3901 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     3961 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     4021 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4081 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4141 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4201 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4261 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     4321 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     4381 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     4441 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4501 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4561 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4621 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4681 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4741 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4801 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4861 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4921 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4981 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5041 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5101 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5161 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5221 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5281 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5341 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5401 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5461 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5521 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5581 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5641 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5701 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5761 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5821 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5881 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5941 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6001 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6901 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6961 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7021 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7081 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     7141 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     7201 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     7261 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     7321 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     7381 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     7441 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7501 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7561 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7621 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7681 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7741 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7801 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7861 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7921 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7981 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     8041 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     8101 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8161 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8221 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8281 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8341 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8401 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8461 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8521 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8581 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8641 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8701 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8761 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8821 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8881 caaccccgag agcgctgccg tctggaccga tggctgtgtg gaagtgctcg ccgatagtgg
     8941 aaacagacgc cccagcactc gtcctagggc aaaggatctg cagtaatgag aattctagat
     9001 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     9061 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     9121 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     9181 ttggcgccgg ctcgaggggg cccggggtta ttaatagtaa tcaattacgg ggtcattagt
     9241 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     9301 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     9361 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     9421 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     9481 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     9541 ctacgtatta gtcatcgcta ttaccatg
//
