LOCUS       sequence-267698-       10831 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of intradomain insertion of truncated human cytosine 
            deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA 
            glycosylase inhibitor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S2 1058 AIDx no UGI
FEATURES             Location/Qualifiers
     source          1..10831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        187..566
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        567..770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     721..741
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     815..834
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        815..833
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      869..878
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4049..4069
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4085..4105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5732..5779
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5780..5803
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5813..5869
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6374..6391)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6380..6604
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6650..7078
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6737..6756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6947..6968
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7087..7107)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7092..7421
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7272..7407
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7334..7353
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7488..8282
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7542..7561)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8152..8171
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8456..8577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8493..8512)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8547..8566
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8626..8642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8626..8642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8639..8661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8650..8666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8674..8680,8681..8698,8699..8704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8719..8740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8857..8874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9028..9616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9108..9127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9787..10578,10579..10647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10410..10429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10648..10752)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(10827..10831,1..15))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
       61 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      121 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      181 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      241 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      301 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      361 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      421 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      481 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      541 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      601 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      661 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      721 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
      781 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      841 aagctggcta gcgtttaaac ttaagcttgc caccatggat aaaaaatata gcattggtct
      901 ggcgattggc accaactccg tgggctgggc tgtgattacc gatgaataca aggttccgag
      961 caagaagttt aaggtgctgg gtaacaccga ccgtcacagc atcaagaaga acctgattgg
     1021 cgctctgctg tttgacagcg gcgagaccgc ggaagccacc cgcctgaagc gcaccgcgcg
     1081 tcgccgttat acccgtcgta agaaccgcat ttgctatctg caagaaatct tcagcaacga
     1141 gatggcgaaa gtggatgaca gctttttcca ccgcctggaa gagagctttc tggtggagga
     1201 agataagaag cacgagcgtc acccgatctt cggtaacatt gtggacgagg tggcctatca
     1261 cgaaaagtat ccgaccatct accacctgcg taaaaaactg gtggatagca ccgacaaagc
     1321 tgatctgcgc ctgatctatc tggctctggc gcacatgatc aaattccgcg gccacttcct
     1381 gattgagggt gacctgaacc cggacaacag cgatgtggat aaactgttta ttcagctggt
     1441 gcagacctat aaccagctgt ttgaagagaa cccgatcaac gcgagcggcg tggacgcgaa
     1501 agctattctg agcgcgcgtc tgagcaagag ccgtcgtctg gaaaacctga tcgcgcagct
     1561 gccgggcgag aagaaaaacg gtctgtttgg caacctgatt gccctgagcc tgggtctgac
     1621 cccgaacttt aagagcaact tcgacctggc ggaggacgcg aaactgcaac tgagcaaaga
     1681 tacctatgac gacgacctgg ataacctgct ggcgcagatc ggcgatcagt atgctgacct
     1741 gtttctggcg gccaaaaacc tgagcgacgc gattctgctg agcgacatcc tgcgtgtgaa
     1801 caccgagatt accaaagcgc cgctgagcgc cagcatgatt aagcgttacg acgaacacca
     1861 ccaggacctg accctgctga aagccctggt gcgccagcag ctgccggaga agtacaaaga
     1921 aatctttttc gatcagagca aaaacggtta tgccggttat attgacggtg gcgcgagcca
     1981 ggaggagttt tataagttca ttaagccgat tctggaaaag atggacggca ccgaggagct
     2041 gctggtgaag ctgaaccgcg aagacctgct gcgcaaacag cgtacctttg ataacggtag
     2101 cattccgcac cagattcacc tgggcgaact gcacgcgatt ctgcgtcgcc aggaggactt
     2161 ctatccgttc ctgaaggata accgtgagaa aattgagaag attctgacct tccgtatccc
     2221 gtactatgtg ggcccgctgg ctcgtggtaa cagccgtttc gcttggatga cccgtaaaag
     2281 cgaggagacc attaccccgt ggaacttcga ggaagtggtg gacaaaggtg ccagcgcgca
     2341 gagctttatc gagcgcatga ccaacttcga caaaaacctg ccgaacgaaa aagtgctgcc
     2401 gaaacacagc ctgctgtatg aatacttcac cgtgtataac gaactgacca aggtgaagta
     2461 tgtgaccgaa ggtatgcgca agccggcttt tctgagcggc gagcagaaga aggcgattgt
     2521 ggacctgctg ttcaagacca accgtaaagt gaccgtgaag cagctgaagg aggactactt
     2581 caagaaaatt gaatgctttg acagcgtgga gatcagcggc gtggaggacc gcttcaacgc
     2641 gagcctgggc acctaccacg atctgctgaa gatcattaag gataaagatt ttctggacaa
     2701 cgaggaaaac gaagacatcc tggaggatat tgtgctgacc ctgaccctgt ttgaggatcg
     2761 tgaaatgatt gaagaacgcc tgaagaccta tgctcacctg ttcgatgata aagtgatgaa
     2821 acagctgaag cgtcgtcgtt ataccggttg gggccgtctg agccgcaaac tgatcaacgg
     2881 cattcgtgac aaacagagcg gcaagaccat cctggatttt ctgaaaagcg atggctttgc
     2941 gaaccgtaac tttatgcagc tgattcacga tgatagcctg accttcaagg aggacattca
     3001 gaaggcgcag gtgagcggcc agggtgacag cctgcacgag cacattgcga acctggcggg
     3061 cagcccggcg attaagaaag gcattctgca aaccgtgaaa gtggtggatg aactggtgaa
     3121 agtgatgggc cgccacaagc cggagaacat tgtgatcgag atggcccgtg agaaccagac
     3181 cacccagaaa ggccagaaga acagccgcga gcgcatgaag cgtattgaag agggtatcaa
     3241 ggagctgggc agccagatcc tgaaggaaca cccggtggaa aacacccagc tgcaaaacga
     3301 gaaactgtat ctgtactacc tgcaaaacgg ccgtgacatg tatgtggatc aggaactgga
     3361 catcaaccgc ctgagcgact acgacgtgga tgcgatcgtg ccgcagagct tcctgaaaga
     3421 cgacagcatt gataacaagg tgctgacccg tagcgacaag aaccgtggca aaagcgataa
     3481 cgtgccgagc gaggaggtgg tgaaaaagat gaaaaactat tggcgccagc tgctgaacgc
     3541 gaagctgatt acccagcgca agttcgacaa cctgaccaag gctgagcgtg gtggcctgag
     3601 cgagctggac aaggctggct ttatcaaacg ccagctggtg gagacccgcc agatcaccaa
     3661 gcacgtggcg cagatcctgg atagccgtat gaacaccaaa tacgatgaga acgataagct
     3721 gatccgcgag gtgaaagtga tcaccctgaa gagcaagctg gtgagcgatt ttcgcaagga
     3781 cttccagttc tacaaggtgc gcgaaatcaa caactaccac cacgcgcacg atgcgtatct
     3841 gaacgcggtg gtgggcaccg ctctgatcaa gaaatatccg aagctggaga gcgagttcgt
     3901 gtacggtgac tataaagtgt acgacgtgcg caaaatgatt gccaagagcg agcaggagat
     3961 tggtaaagct accgctaaat atttctttta cagcaacatc atgaacttct ttaagaccga
     4021 gatcaccctg gccaacggtg aaatccgtcc gaagaagaaa cgtaaggtgg gtagcagcgg
     4081 tagcccgaaa aagaagcgca aagtgggcag cgacgcgctg gacgatttcg atctggacat
     4141 gctgggcagc gatgcgctgg atgatttcgg cggtggcagc atggatagcc tgctgatgaa
     4201 ccgtcgcaag ttcctgtacc agttcaaaaa cgtgcgttgg gcgaagggtc gtcgtgagac
     4261 ctacctgtgc tacgtggtga agcgtcgtga cagcgcgacc agcttcagcc tggattttgg
     4321 ctatctgcgt aacaaaaacg gttgccacgt ggaactgctg tttctgcgct acattagcga
     4381 ctgggacctg gaccccggtc gctgctaccg cgtgacctgg tttaccagct ggagcccgtg
     4441 ctatgattgc gcgcgtcacg tggccgattt cctgcgcggt aacccgaacc tgagcctgcg
     4501 cattttcacc gcgcgcctgt atttctgcga ggatcgcaag gcggagccgg agggcctgcg
     4561 tcgtctgcac cgtgctggtg tgcagattgc gattatgacc tttaaggact acttttattg
     4621 ctggaacacc tttgtggaga accacgagcg caccttcaaa gcgtgggagg gtctgcacga
     4681 gaacagcgtg cgtctgagcc gccagctgcg tcgcatcctg ctgccgggcg gtagcggcgg
     4741 tagcggtggt agcggcggca gcggtggtag cggtggcagc ggtggtcgca aacgcccgct
     4801 gatcgaaacc aacggcgaaa ccggtgaaat cgtgtgggat aagggtcgcg atttcgcgac
     4861 cgtgcgcaag gtgctgagca tgccgcaggt gaacatcgtg aagaagaccg aagtgcagac
     4921 cggcggtttt agcaaggaaa gcatcctgcc gaagcgcaac agcgataagc tgatcgcgcg
     4981 taagaaggac tgggacccga aaaagtatgg tggcttcgac agcccgaccg tggcgtacag
     5041 cgtgctggtg gtggccaagg tggaaaaggg caaaagcaaa aaactgaaaa gcgtgaagga
     5101 gctgctgggt attaccatta tggaacgtag cagctttgag aagaacccga tcgacttcct
     5161 ggaggcgaag ggttacaagg aagtgaagaa agatctgatc atcaaactgc cgaagtacag
     5221 cctgtttgag ctggaaaacg gccgtaagcg tatgctggcg agcgcgggtg agctgcaaaa
     5281 aggtaacgag ctggcgctgc cgagcaagta cgtgaacttt ctgtacctgg cgagccacta
     5341 tgaaaaactg aaaggcagcc cggaggacaa cgagcagaag cagctgtttg tggagcagca
     5401 caagcactat ctggacgaaa tcatcgaaca gatcagcgag ttcagcaaac gcgtgattct
     5461 ggccgacgcc aacctggaca aagtgctgag cgcctacaac aagcaccgcg ataaaccgat
     5521 tcgcgaacag gccgagaaca tcattcacct gtttaccctg accaacctgg gcgcgccggc
     5581 ggcgttcaaa tactttgata ccaccattga tcgcaaacgc tacaccagca ccaaggaagt
     5641 gctggatgcg accctgatcc accagagcat taccggtctg tacgaaaccc gcatcgatct
     5701 gagccagctg ggcggcgata gcggtggtag caagcgtccg gcggcgacca aaaaggccgg
     5761 ccaggcgaag aagaagaagg actacaagga tgacgatgac aaaggcagcg gcgccaccaa
     5821 cttcagcctg ctgaagcagg ccggtgacgt ggaagaaaac cccggcccga tggcgaagcc
     5881 gctgagccag gaggaaagca ccctgattga gcgcgccacc gcgaccatca acagcattcc
     5941 gattagcgaa gactacagcg tggcgagcgc cgctctgagc agcgatggcc gtatcttcac
     6001 cggcgtgaac gtgtaccact ttaccggcgg tccgtgcgcg gaactggtgg tgctgggcac
     6061 cgcggcggct gcggctgcgg gtaacctgac ctgcatcgtg gccattggta acgaaaaccg
     6121 cggcattctg agcccgtgcg gccgttgccg ccaggtgctg ctggacctgc accccggtat
     6181 caaagcgatt gtgaaagata gcgatggtca accgaccgct gtgggtatcc gtgaactgct
     6241 gccatccggc tatgtttggg agggctaagg taccgagctc ggatccacta gtccagtgtg
     6301 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
     6361 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6421 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6481 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6541 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6601 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     6661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6781 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6841 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6901 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6961 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7021 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     7081 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7141 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7201 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7261 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7321 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     7381 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     7441 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     7501 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     7561 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     7621 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     7681 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     7741 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     7801 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     7861 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     7921 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     7981 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     8041 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     8101 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     8161 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     8221 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     8281 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     8341 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     8401 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     8461 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8521 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8581 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     8641 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8701 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8761 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8821 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    10021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    10081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
    10801 cccgatcccc tatggtgcac tctcagtaca a
//
