LOCUS       sequence-267706-       11377 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of intradomain insertion of ABEMax (wildtype Tada monomer
            coupled with evolved Tada monomer) at position 1058 of nSpyCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11377)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 11377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S2 1058 ABEMax
FEATURES             Location/Qualifiers
     source          1..11377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        137..516
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        517..720
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     671..691
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     765..784
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        765..783
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      819..828
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3999..4019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4035..4055
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6228..6275
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6276..6299
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6309..6365
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6870..6887)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6876..7100
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7146..7574
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7233..7252)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7443..7464
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7583..7603)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7588..7917
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7768..7903
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7830..7849
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7984..8778
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8038..8057)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8648..8667
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8952..9073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8989..9008)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9043..9062
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9122..9138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9122..9138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9135..9157)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9146..9162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9170..9176,9177..9194,9195..9200))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9215..9236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9353..9370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9524..10112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9604..9623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10283..11074,11075..11143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10906..10925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11144..11248)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11323..11342)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
       61 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
      121 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      181 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      241 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      301 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      361 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      421 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      481 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      541 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      601 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      661 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      721 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
      781 agggagaccc aagctggcta gcgtttaaac ttaagcttgc caccatggat aaaaaatata
      841 gcattggtct ggcgattggc accaactccg tgggctgggc tgtgattacc gatgaataca
      901 aggttccgag caagaagttt aaggtgctgg gtaacaccga ccgtcacagc atcaagaaga
      961 acctgattgg cgctctgctg tttgacagcg gcgagaccgc ggaagccacc cgcctgaagc
     1021 gcaccgcgcg tcgccgttat acccgtcgta agaaccgcat ttgctatctg caagaaatct
     1081 tcagcaacga gatggcgaaa gtggatgaca gctttttcca ccgcctggaa gagagctttc
     1141 tggtggagga agataagaag cacgagcgtc acccgatctt cggtaacatt gtggacgagg
     1201 tggcctatca cgaaaagtat ccgaccatct accacctgcg taaaaaactg gtggatagca
     1261 ccgacaaagc tgatctgcgc ctgatctatc tggctctggc gcacatgatc aaattccgcg
     1321 gccacttcct gattgagggt gacctgaacc cggacaacag cgatgtggat aaactgttta
     1381 ttcagctggt gcagacctat aaccagctgt ttgaagagaa cccgatcaac gcgagcggcg
     1441 tggacgcgaa agctattctg agcgcgcgtc tgagcaagag ccgtcgtctg gaaaacctga
     1501 tcgcgcagct gccgggcgag aagaaaaacg gtctgtttgg caacctgatt gccctgagcc
     1561 tgggtctgac cccgaacttt aagagcaact tcgacctggc ggaggacgcg aaactgcaac
     1621 tgagcaaaga tacctatgac gacgacctgg ataacctgct ggcgcagatc ggcgatcagt
     1681 atgctgacct gtttctggcg gccaaaaacc tgagcgacgc gattctgctg agcgacatcc
     1741 tgcgtgtgaa caccgagatt accaaagcgc cgctgagcgc cagcatgatt aagcgttacg
     1801 acgaacacca ccaggacctg accctgctga aagccctggt gcgccagcag ctgccggaga
     1861 agtacaaaga aatctttttc gatcagagca aaaacggtta tgccggttat attgacggtg
     1921 gcgcgagcca ggaggagttt tataagttca ttaagccgat tctggaaaag atggacggca
     1981 ccgaggagct gctggtgaag ctgaaccgcg aagacctgct gcgcaaacag cgtacctttg
     2041 ataacggtag cattccgcac cagattcacc tgggcgaact gcacgcgatt ctgcgtcgcc
     2101 aggaggactt ctatccgttc ctgaaggata accgtgagaa aattgagaag attctgacct
     2161 tccgtatccc gtactatgtg ggcccgctgg ctcgtggtaa cagccgtttc gcttggatga
     2221 cccgtaaaag cgaggagacc attaccccgt ggaacttcga ggaagtggtg gacaaaggtg
     2281 ccagcgcgca gagctttatc gagcgcatga ccaacttcga caaaaacctg ccgaacgaaa
     2341 aagtgctgcc gaaacacagc ctgctgtatg aatacttcac cgtgtataac gaactgacca
     2401 aggtgaagta tgtgaccgaa ggtatgcgca agccggcttt tctgagcggc gagcagaaga
     2461 aggcgattgt ggacctgctg ttcaagacca accgtaaagt gaccgtgaag cagctgaagg
     2521 aggactactt caagaaaatt gaatgctttg acagcgtgga gatcagcggc gtggaggacc
     2581 gcttcaacgc gagcctgggc acctaccacg atctgctgaa gatcattaag gataaagatt
     2641 ttctggacaa cgaggaaaac gaagacatcc tggaggatat tgtgctgacc ctgaccctgt
     2701 ttgaggatcg tgaaatgatt gaagaacgcc tgaagaccta tgctcacctg ttcgatgata
     2761 aagtgatgaa acagctgaag cgtcgtcgtt ataccggttg gggccgtctg agccgcaaac
     2821 tgatcaacgg cattcgtgac aaacagagcg gcaagaccat cctggatttt ctgaaaagcg
     2881 atggctttgc gaaccgtaac tttatgcagc tgattcacga tgatagcctg accttcaagg
     2941 aggacattca gaaggcgcag gtgagcggcc agggtgacag cctgcacgag cacattgcga
     3001 acctggcggg cagcccggcg attaagaaag gcattctgca aaccgtgaaa gtggtggatg
     3061 aactggtgaa agtgatgggc cgccacaagc cggagaacat tgtgatcgag atggcccgtg
     3121 agaaccagac cacccagaaa ggccagaaga acagccgcga gcgcatgaag cgtattgaag
     3181 agggtatcaa ggagctgggc agccagatcc tgaaggaaca cccggtggaa aacacccagc
     3241 tgcaaaacga gaaactgtat ctgtactacc tgcaaaacgg ccgtgacatg tatgtggatc
     3301 aggaactgga catcaaccgc ctgagcgact acgacgtgga tgcgatcgtg ccgcagagct
     3361 tcctgaaaga cgacagcatt gataacaagg tgctgacccg tagcgacaag aaccgtggca
     3421 aaagcgataa cgtgccgagc gaggaggtgg tgaaaaagat gaaaaactat tggcgccagc
     3481 tgctgaacgc gaagctgatt acccagcgca agttcgacaa cctgaccaag gctgagcgtg
     3541 gtggcctgag cgagctggac aaggctggct ttatcaaacg ccagctggtg gagacccgcc
     3601 agatcaccaa gcacgtggcg cagatcctgg atagccgtat gaacaccaaa tacgatgaga
     3661 acgataagct gatccgcgag gtgaaagtga tcaccctgaa gagcaagctg gtgagcgatt
     3721 ttcgcaagga cttccagttc tacaaggtgc gcgaaatcaa caactaccac cacgcgcacg
     3781 atgcgtatct gaacgcggtg gtgggcaccg ctctgatcaa gaaatatccg aagctggaga
     3841 gcgagttcgt gtacggtgac tataaagtgt acgacgtgcg caaaatgatt gccaagagcg
     3901 agcaggagat tggtaaagct accgctaaat atttctttta cagcaacatc atgaacttct
     3961 ttaagaccga gatcaccctg gccaacggtg aaatccgtcc gaagaagaaa cgtaaggtgg
     4021 gtagcagcgg tagcccgaaa aagaagcgca aagtgggcag cgacgcgctg gacgatttcg
     4081 atctggacat gctgggcagc gatgcgctgg atgatttcgg cggtggcagc tctgaagtcg
     4141 agtttagcca cgagtattgg atgaggcacg cactgaccct ggcaaagcga gcatgggatg
     4201 aaagagaagt ccccgtgggc gccgtgctgg tgcacaacaa tagagtgatc ggagagggat
     4261 ggaacaggcc aatcggccgc cacgacccta ccgcacacgc agagatcatg gcactgaggc
     4321 agggaggcct ggtcatgcag aattaccgcc tgatcgatgc caccctgtat gtgacactgg
     4381 agccatgcgt gatgtgcgca ggagcaatga tccacagcag gatcggaaga gtggtgttcg
     4441 gagcacggga cgccaagacc ggcgcagcag gctccctgat ggatgtgctg caccaccccg
     4501 gcatgaacca ccgggtggag atcacagagg gaatcctggc agacgagtgc gccgccctgc
     4561 tgagcgattt ctttagaatg cggagacagg agatcaaggc ccagaagaag gcacagagct
     4621 ccaccgactc tggaggatct agcggaggat cctctggaag cgagacacca ggcacaagcg
     4681 agtccgccac accagagagc tccggcggct cctccggagg atcctctgag gtggagtttt
     4741 cccacgagta ctggatgaga catgccctga ccctggccaa gagggcacgc gatgagaggg
     4801 aggtgcctgt gggagccgtg ctggtgctga acaatagagt gatcggcgag ggctggaaca
     4861 gagccatcgg cctgcacgac ccaacagccc atgccgaaat tatggccctg agacagggcg
     4921 gcctggtcat gcagaactac agactgattg acgccaccct gtacgtgaca ttcgagcctt
     4981 gcgtgatgtg cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg tttggcgtga
     5041 ggaacgcaaa aaccggcgcc gcaggctccc tgatggacgt gctgcactac cccggcatga
     5101 atcaccgcgt cgaaattacc gagggaatcc tggcagatga atgtgccgcc ctgctgtgct
     5161 atttctttcg gatgcctaga caggtgttca atgctcagaa gaaggcccag agctccaccg
     5221 acggcggtag cggcggtagc ggtggtagcg gcggcagcgg tggtagcggt ggcagcggtg
     5281 gtcgcaaacg cccgctgatc gaaaccaacg gcgaaaccgg tgaaatcgtg tgggataagg
     5341 gtcgcgattt cgcgaccgtg cgcaaggtgc tgagcatgcc gcaggtgaac atcgtgaaga
     5401 agaccgaagt gcagaccggc ggttttagca aggaaagcat cctgccgaag cgcaacagcg
     5461 ataagctgat cgcgcgtaag aaggactggg acccgaaaaa gtatggtggc ttcgacagcc
     5521 cgaccgtggc gtacagcgtg ctggtggtgg ccaaggtgga aaagggcaaa agcaaaaaac
     5581 tgaaaagcgt gaaggagctg ctgggtatta ccattatgga acgtagcagc tttgagaaga
     5641 acccgatcga cttcctggag gcgaagggtt acaaggaagt gaagaaagat ctgatcatca
     5701 aactgccgaa gtacagcctg tttgagctgg aaaacggccg taagcgtatg ctggcgagcg
     5761 cgggtgagct gcaaaaaggt aacgagctgg cgctgccgag caagtacgtg aactttctgt
     5821 acctggcgag ccactatgaa aaactgaaag gcagcccgga ggacaacgag cagaagcagc
     5881 tgtttgtgga gcagcacaag cactatctgg acgaaatcat cgaacagatc agcgagttca
     5941 gcaaacgcgt gattctggcc gacgccaacc tggacaaagt gctgagcgcc tacaacaagc
     6001 accgcgataa accgattcgc gaacaggccg agaacatcat tcacctgttt accctgacca
     6061 acctgggcgc gccggcggcg ttcaaatact ttgataccac cattgatcgc aaacgctaca
     6121 ccagcaccaa ggaagtgctg gatgcgaccc tgatccacca gagcattacc ggtctgtacg
     6181 aaacccgcat cgatctgagc cagctgggcg gcgatagcgg tggtagcaag cgtccggcgg
     6241 cgaccaaaaa ggccggccag gcgaagaaga agaaggacta caaggatgac gatgacaaag
     6301 gcagcggcgc caccaacttc agcctgctga agcaggccgg tgacgtggaa gaaaaccccg
     6361 gcccgatggc gaagccgctg agccaggagg aaagcaccct gattgagcgc gccaccgcga
     6421 ccatcaacag cattccgatt agcgaagact acagcgtggc gagcgccgct ctgagcagcg
     6481 atggccgtat cttcaccggc gtgaacgtgt accactttac cggcggtccg tgcgcggaac
     6541 tggtggtgct gggcaccgcg gcggctgcgg ctgcgggtaa cctgacctgc atcgtggcca
     6601 ttggtaacga aaaccgcggc attctgagcc cgtgcggccg ttgccgccag gtgctgctgg
     6661 acctgcaccc cggtatcaaa gcgattgtga aagatagcga tggtcaaccg accgctgtgg
     6721 gtatccgtga actgctgcca tccggctatg tttgggaggg ctaaggtacc gagctcggat
     6781 ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc
     6841 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     6901 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6961 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7021 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7081 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     7141 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7201 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7261 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7321 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7381 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7441 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     7501 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     7561 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     7621 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     7681 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7741 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7801 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     7861 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7921 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     7981 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     8041 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     8101 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     8161 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     8221 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     8281 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     8341 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     8401 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     8461 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     8521 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     8581 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     8641 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     8701 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     8761 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     8821 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8881 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8941 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9001 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9061 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     9121 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9181 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9241 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9301 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9361 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9421 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9481 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9541 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9601 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9661 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9721 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9781 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9841 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9901 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9961 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10021 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10081 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10141 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10201 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10261 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10321 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10381 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10441 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10501 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10561 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10621 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10681 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10741 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10801 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10861 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10921 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10981 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    11041 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    11101 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    11161 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11221 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    11281 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    11341 cgcatagtta agccagtatc tgctccctgc ttgtgtg
//
