LOCUS       sequence-267995-        1273 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pSECC-sgSIK2-B
FEATURES             Location/Qualifiers
     source          1..1273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        72..147
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    195..312
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        411..622
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      667..676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             676..699
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             706..726
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cccgtaattc gatttcttgg ctttatatat cttgtggaag gacgaaacac cggtggggtt
       61 ctacgacatc ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      121 cttgaaaaag tggcaccgag tcggtgcttt tttgaattct agatcttgag acaaatggca
      181 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      241 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      301 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccggcaaacc
      361 gctgttccta ggaatcccga ggccttcgag tggctccggt gcccgtcagt gggcagagcg
      421 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
      481 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
      541 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
      601 cgggtttgcc gccagaacac aggtgtcgtg acgcgggacc cgacattagc gctacagctt
      661 aagcgggcca ccatggatta caaagacgat gacgataaga tggccccaaa gaagaagcgg
      721 aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg cctggacatc
      781 ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa
      841 ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg
      901 ctgttcgaca gcggcgaaac agccgaggcc acccggctga gagaccgcca gaaggaagat
      961 acaccagacg gaagaaccgg atctgctatc tgcaagagat tcttcagcaa cgagatggca
     1021 agtggacgac agcttcttca cagactgaga gtccttcctg gtgagagata gagcacgagc
     1081 ggcaccatct tcggcacatc gtggacgagt gctaccacag agtaccccac catctacact
     1141 ggnangacct gtgacagcac ggaccagcga cctggcgctg atcttatctn ncctnnccaa
     1201 tgatcannnc ngncnnnctg aatcaggcga actgaaaccg acaaactacg gcgtacgtgg
     1261 acacagccgt ggt
//
