LOCUS       sequence-26817-e         841 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Stelnicki EJ, Arbeit J, Cass DL, Saner C, Harrison M, Largman C
  TITLE     Modulation of the human homeobox genes PRX-2 and HOXB13 in scarless 
            fetal wounds.
  JOURNAL   J Invest Dermatol 1998 Jul;111(1):57-63.
  PUBMED    9665387
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Invest 
            Dermatol"; date: "1998-07"; volume: "111(1)"; pages: "57-63"
COMMENT     Sequence Label: PRRX2-pET
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             24..46
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             65..82
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..109
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             113..145
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tgnnnnnntc cctctagaat aattttgttt aactttaaga aggagatata ccatgggcag
       61 cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatggctag
      121 catgactggt ggacagcaaa tgggtcggga tccgaattcg agctccaccg cggtggcggc
      181 cgccgccttc gccctagacc cgccagcgcc cggcccgggg cccccgcccg cacccggcga
      241 ttgcgcccag gcgcgcaaga acttctcggt gagccacctc ctggacctgg aggaggtggc
      301 ggctgctggg cgcagggctg cggggcccgt ttcggggccc gccgaggcgc gggagggagc
      361 ggcgcgcgaa ccgtccgggg gcagcagcgg cagcgaggcg gcgccgcagg acggtgactg
      421 tcccagcccc ggccgtggca ccaaacgaaa gaagaagcag cgccggaatc gaaccacatt
      481 caacagcagc cagctgcagg cgctggagcg tgtatttgag cgcacacact accctgacgc
      541 ctttgtgcgt gaagagctag ctcgccgtgt caacctcagt gaggcacgtg tccaagtctg
      601 gttccagaac cgccgtgcca agttttcncc ggaatgaacg tgccatgctg gctacccgct
      661 ctgcctcgtt gctcaagtct tatggccagg aggcggccat tgtagaacan gcgggtgncc
      721 cccccaacct accacnatga gcnnnanatt atcgtatcct gncagcatcc nnccccctac
      781 agctccgngn cctccctata ncccccggag gttcaagttc ctnncnaact tctnnnnnnn
      841 c
//
