LOCUS       sequence-268361-        2031 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Constitutive expression of VPR-FRB under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD355 VPR-FRB (NLS)
FEATURES             Location/Qualifiers
     source          1..2031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             121..270
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             727..1083
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             1102..1671
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatca
      121 gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac
      181 cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac
      241 gcccttgatg atttcgacct ggacatgctg attaactcta gaagttccgg atctgttggt
      301 agccagtacc tgcccgacac cgacgaccgg caccggatcg aggaaaagcg gaagcggacc
      361 tacgagacat tcaagagcat catgaagaag tcccccttca gcggccccac cgaccctaga
      421 cctccaccta gaagaatcgc cgtgcccagc agatccagcg ccagcgtgcc aaaacctgcc
      481 ccccagcctt accccttcac cagcagcctg agcaccatca actacgacga gttccctacc
      541 atggtgttcc ccagcggcca gatctctcag gcctctgctc tggctccagc ccctcctcag
      601 gtgctgcctc aggctcctgc tcctgcacca gctccagcca tggtgtctgc actggctcag
      661 gcaccagcac ccgtgcctgt gctggctcct ggacctccac aggctgtggc tccaccagcc
      721 cctaaaccta cacaggccgg cgagggcaca ctgtctgaag ctctgctgca gctgcagttc
      781 gacgacgagg atctgggagc cctgctggga aacagcaccg atcctgccgt gttcaccgac
      841 ctggccagcg tggacaacag cgagttccag cagctgctga accagggcat ccctgtggcc
      901 cctcacacca ccgagcccat gctgatggaa taccccgagg ccatcacccg gctcgtgaca
      961 ggcgctcaga ggcctcctga tccagctcct gcccctctgg gagcaccagg cctgcctaat
     1021 ggactgctgt ctggcgacga ggacttcagc tctatcgccg atatggattt ctcagccttg
     1081 ctgggctctg gcagcggcag ccgggattcc agggaaggga tgtttttgcc gaagcctgag
     1141 gccggctccg ctattagtga cgtgtttgag ggccgcgagg tgtgccagcc aaaacgaatc
     1201 cggccatttc atcctccagg aagtccatgg gccaaccgcc cactccccgc cagcctcgca
     1261 ccaacaccaa ccggtccagt acatgagcca gtcgggtcac tgaccccggc accagtccct
     1321 cagccactgg atccagcgcc cgcagtgact cccgaggcca gtcacctgtt ggaggatccc
     1381 gatgaagaga cgagccaggc tgtcaaagcc cttcgggaga tggccgatac tgtgattccc
     1441 cagaaggaag aggctgcaat ctgtggccaa atggaccttt cccatccgcc cccaaggggc
     1501 catctggatg agctgacaac cacacttgag tccatgaccg aggatctgaa cctggactca
     1561 cccctgaccc cggaattgaa cgagattctg gataccttcc tgaacgacga gtgcctcttg
     1621 catgccatgc atatcagcac aggactgtcc atcttcgaca catctctgtt ttctggttct
     1681 ggttctggtt caggatccgg tggtggcggg tctggaggag gaggtagtgg tggaggtggc
     1741 tctggtacca tgcttgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt
     1801 ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa
     1861 cggggacccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg
     1921 gaggcccaag agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcctccaa
     1981 gcctgggacc tctattatca tgtgttccga cgaatctcaa agactagatg a
//
