LOCUS       sequence-268623-        8950 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Kim DS, Matsuda T, Cepko CL
  TITLE     A core paired-type and POU homeodomain-containing transcription 
            factor program drives retinal bipolar cell gene expression.
  JOURNAL   J Neurosci. 2008 Jul 30. 28(31):7748-64.
  PUBMED    18667607
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Jul 30. 28(31):7748-64."
COMMENT     Sequence Label: Chx10 (2.5kb distal 5' reg)+SV40p-tdTomato-IRE...
FEATURES             Location/Qualifiers
     source          1..8950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1521..1717
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1568..1703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      1756..1765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1762..3192
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    3202..3754
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3346..3363)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3573..3592
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3766..5265
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     primer_bind     complement(5760..5779)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5825..5880
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5879..5898)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6241..6257)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6241..6257)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6254..6276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6265..6281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6289..6295,6296..6313,6314..6319))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6334..6355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6414..6609
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6460..6595
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6615..6749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6652..6671)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6706..6725
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6817..6834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6988..7576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7068..7087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7747..8538,8539..8607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8370..8389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8608..8712)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttttttttt tttttttttt tttttttttt tgacaaagtc tcattgtata cccctggcta
       61 gcctagagct taatatgtag accaggctgt ccttgaactc acagaaaccc tcccttctct
      121 gcctcccagg cgctgggatt aaattatgta cctccacatc tggatggttt ctcctcttgc
      181 tattgggtgg acagacaggc ttacaaagga ctctttcaca tcccacgagg catgacaggc
      241 ccatgagaca ggtacaactc cggttctcag gtctgggcag tgcatcccct gtggtgggtg
      301 ggtgtggaca tcttccaggc cccaggacac accaggaatc aagagggagg ttgccagagg
      361 aaacaggact aaccagaagg gccaaagagg tgtagcggct agtctgagca agcagggcca
      421 gcatggatca gtctgtaaag aaaatggctt gtagcactga gggtcggatc ttccccagac
      481 accgggggct ggagagaggt gggcaaggtg tttgttgatt ggccagctga gatttttcca
      541 gactttccta gtcatcaaaa tagtcttttg agctgagcag tggtggcaca ggaacttaat
      601 ctgaacactc aggaggcaga ggcaggagga tctctgtgag ttctgaggcc aacctggtct
      661 acagagtgag gtccaggata accagggtta cacagagaaa ccctgtcttg gaaaaaaaaa
      721 aaaaccaaac agacagatgg cagaatattt gcatgtaatt catgtatgtc cctcgggcca
      781 cttcagctcc tctagtgata actacgacga tgtgagcatg gtgtaaactg tatgcttata
      841 ttatcattac tttatactgt attattaaat gacaagaaaa atctgtccat gttccctgaa
      901 ggtttgacaa ggggatcagt ctcccaatca ggaagttcct gtctcctccc tgtcctcctt
      961 ctcaggcctg ggatgggctg actggtgcca ttttccccca gcacacctga taccatcagc
     1021 atcctctggc ccagtctggt ctcttcagtg tcccccatgg atctcagaac acatccatcc
     1081 atattgcagt ggggagatgg gggtagggga agctcccagg cctgtctagg ctatgggata
     1141 atttgatatt caaatcctgg caaagacctg cttcaaattg ctacaaatta agctgatttt
     1201 ggccctctgg atgaaggatt tgctccggtg gatcagcctt cccagggctt gatgaggtca
     1261 gattacagaa ggagggacaa gagaaagaaa cggggggggg gggctcccca tctgagagtt
     1321 tatcagaacc taatccgggg agaagagcac tggctggggc tgcttgcccg ctaatcccag
     1381 ctgccattaa aatattaaag ataaatctaa tcgtctcttt atccaaaata agcgactttt
     1441 gtgtggggag aaaacgtcta accccttagg aggagaatta gttctaatgc atcaaatgga
     1501 attgctcgag atctgcgatc tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     1561 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atcgctgact
     1621 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     1681 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggtaccg cgggcccggg
     1741 atccaccggg atcccgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg
     1801 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     1861 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     1921 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     1981 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     2041 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     2101 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     2161 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     2221 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     2281 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     2341 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     2401 cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac cggcagcacc
     2461 ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt catcaaagag
     2521 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
     2581 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     2641 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
     2701 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
     2761 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
     2821 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
     2881 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
     2941 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
     3001 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
     3061 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
     3121 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag
     3181 ctgtacaagt aaagcsccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     3241 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     3301 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     3361 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     3421 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     3481 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     3541 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     3601 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     3661 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     3721 acgtggtttt cctttgaaaa acacgatgat aatatggggt cgaggctgct gctgctgctg
     3781 ctgctgggcc tgaggctaca gctctccctg ggcatcatcc cagttgagga ggagaacccg
     3841 gacttctgga accgcgaggc agccgaggcc ctgggtgccg ccaagaagct gcagcctgca
     3901 cagacagccg ccaagaacct catcatcttc ctgggcgatg ggatgggggt gtctacggtg
     3961 acagctgcca ggatcctaaa agggcagaag aaggacaaac tggggcctga gatacccctg
     4021 gccatggacc gcttcccata tgtggctctg tccaagacat acaatgtaga caaacatgtg
     4081 ccagacagtg gagccacagc cacggcctac ctgtgcgggg tcaagggcaa cttccagacc
     4141 attggcttga gtgcagccgc ccgctttaac cagtgcaaca cgacacgcgg caacgaggtc
     4201 atctccgtga tgaatcgggc caagaaagca gggaagtcag tgggagtggt aaccaccaca
     4261 cgagtgcagc acgcctcgcc agccggcacc tacgcccaca cggtgaaccg caactggtac
     4321 tcggacgccg acgtgcctgc ctcggcccgc caggaggggt gccaggacat cgctacgcag
     4381 ctcatctcca acatggacat tgacgtgatc ctaggtggag gccgaaagta catgtttcgc
     4441 atgggaaccc cagaccctga gtacccagat gactacagcc aaggtgggac caggctggac
     4501 gggaagaatc tggtgcagga atggctggcg aagcgccagg gtgcccggta tgtgtggaac
     4561 cgcactgagc tcatgcaggc ttccctggac ccgtctgtga cccatctcat gggtctcttt
     4621 gagcctggag acatgaaata cgagatccac cgagactcca cactggaccc ctccctgatg
     4681 gagatgacag aggctgccct gcgcctgctg agcaggaacc cccgcggctt cttcctcttc
     4741 gtggagggtg gtcgcatcga ccatggtcat catgaaagca gggcttaccg ggcactgact
     4801 gagacgatca tgttcgacga cgccattgag agggcgggcc agctcaccag cgaggaggac
     4861 acgctgagcc tcgtcactgc cgaccactcc cacgtcttct ccttcggagg ctaccccctg
     4921 cgagggagct ccatcttcgg gctggcccct ggcaaggccc gggacaggaa ggcctacacg
     4981 gtcctcctat acggaaacgg tccaggctat gtgctcaagg acggcgcccg gccggatgtt
     5041 accgagagcg agagcgggag ccccgagtat cggcagcagt cagcagtgcc cctggacgaa
     5101 gagacccacg caggcgagga cgtggcggtg ttcgcgcgcg gcccgcaggc gcacctggtt
     5161 cacggcgtgc aggagcagac cttcatagcg cacgtcatgg ccttcgccgc ctgcctggag
     5221 ccctacaccg cctgcgacct ggcgcccccc gccggcacca ccgacgccgc gcacccgggg
     5281 cggtccgtgg tccccgcgtt gcttcctctg ctggccggga ccctgctgct gctggagacg
     5341 gccactgctc cctgagtgtc ccgtccctgg ggctcctgct tccccatccc ggagttctcc
     5401 tgctccccac ctcctgtcgt cctgcctggc ctccagcccg agtcgtcatc cccggagtcc
     5461 ctatacagag gtcctgccat ggaaccttcc cctccccgtg cgctctgggg actgagccca
     5521 tgacaccaaa cctgcccctt ggctgctctc ggactcccta ccccaacccc agggactgca
     5581 ggttgtgccc tgtggctgcc tgcaccccag gaaaggaggg ggctcaggcc atccagccac
     5641 cacctacagc ccagtggggt cgaggatatc gcggccggcc gcactcctca ggtgcaggct
     5701 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     5761 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     5821 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     5881 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     5941 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     6001 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     6061 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     6121 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     6181 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg
     6241 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
     6421 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6481 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6541 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6601 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6661 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6721 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
     6781 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8041 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8341 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8701 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacaattc aaggaatggc
     8761 ttttttttaa agatttattt atttattata tgtaagtaca ctgtagctgt cttcagacac
     8821 tccagaagag ggcgtgagat ctcattacag atggttgtga gccaccatgt ggttgctggg
     8881 atttgaactc aggaccttcg gaagagcatt cagtgctctt aaccgctgag ccatctctcc
     8941 aggccccggc
//
