LOCUS       sequence-269480-        9458 bp    DNA     circular SYN 14-APR-2025
DEFINITION  FRET biosensor for ATP measurements in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Botman D, van Heerden JH, Teusink B
  TITLE     An Improved ATP FRET Sensor For Yeast Shows Heterogeneity During 
            Nutrient Transitions.
  JOURNAL   ACS Sens. 2020 Mar 27;5(3):814-822. doi: 10.1021/acssensors.9b02475.
            Epub 2020 Mar 3.
  PUBMED    32077276
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.9b02475"; journalName: "ACS Sens"; date: 
            "2020-03-27- 27"; volume: "5"; issue: "3"; pages: "814-822"
COMMENT     Sequence Label: pDRF1-GW yAT1.03
FEATURES             Location/Qualifiers
     source          1..9458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1909..3339
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(3348..3372)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3434..3453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3435..3453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3601..3788
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3656..3680)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3800..3817)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3800..3816)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3809..3831)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4252..5598
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5709..6512)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6513..6728)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6753..6771)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6839..6943
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6944..7012,7013..7804)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7162..7181)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7975..8563
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8464..8483
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8717..8734
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8851..8872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8887..8892,8893..8910,8911..8917)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8925..8941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8930..8952
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8949..8965
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8949..8965
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8995..9450)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9132..9153)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9344..9363
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgctagc actagtaagc ttttaattaa atggttagta aaggtgaaga attgttcact
      841 ggtgtagtgc caattttagt agaattagat ggtgacgtta acggtcataa gttctctgta
      901 agcggtgaag gtgaaggtga cgcaacatac ggtaaattaa ctttgaaatt catttgtact
      961 acaggtaaat taccagttcc atggccaact ttggttacta ctttgtcttg gggtgttcaa
     1021 tgtttcgcta gatacccaga tcatatgaag caacatgatt tctttaagtc tgctatgcca
     1081 gaaggttacg ttcaagaaag aactatcttc ttcaaggatg atggtaacta taagactaga
     1141 gctgaagtta agttcgaagg agatacttta gttaatagaa ttgaattgaa gggtattgat
     1201 tttaaggaag atggtaatat tttgggtcat aagttggaat ataattactt ctctgataac
     1261 gtttacatta ctgctgataa gcaaaagaac ggtattaagg cgaacttcaa gattagacat
     1321 aacattgaag atggtggtgt tcaattagct gatcattacc aacaaaacac tccaattggt
     1381 gacggtccag ttttgttgcc agataaccat tacttgtcta ctcaatctaa gttgtctaag
     1441 gatccaaacg aaaagagaga tcatatggtt ttgttggaat tcgttactgc tgctatcgat
     1501 atgaaaactg tgaaagtgaa tataacaacc cctgatgggc cagtctacga cgctgatatc
     1561 gagatggtgt ccgtgcgggc cgagagtggt gatctcggca tcctccccgg tcacattccc
     1621 acaaaggccc cactgaagat cggagctgtg cggctgaaga aggacggcca aaccgagatg
     1681 gtcgcagtct caggcggcac tgttgaagtg cggcctgacc acgttaccat taatgctcaa
     1741 gccgctgaaa cagccgaagg aatcgacaaa gagagagcag aagccgcaag acagagggcc
     1801 caggagcggc tgaactctca atccgatgac accgatattc gccgggccga gctggcactg
     1861 cagagggccc tgaacagact ggacgtggct gggaaggcaa acgaattcat ggtgagcaag
     1921 ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     1981 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     2041 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     2101 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     2161 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     2221 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     2281 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     2341 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     2401 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     2461 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     2521 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     2581 ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc ctcctccgag
     2641 gacaacaaca tggccgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     2701 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2761 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     2821 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     2881 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2941 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     3001 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     3061 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     3121 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     3181 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     3241 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     3301 cacctgttcc tgtacggcat ggacgagctg tacaagtaag cggccgcacc cagctttctt
     3361 gtacaaagtg gtgtagttaa ttcatgatgg ccgctgcagg tcgacctcga gggggggccc
     3421 ggtacccaat tcgccctata gtgagtcgta ttacgcgcgg atccagcttt ggacttcttc
     3481 gccagaggtt tggtcaagtc tccaatcaag gttgtcggct tgtctacctt gccagaaatt
     3541 tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa
     3601 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta
     3661 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct
     3721 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc
     3781 tctaccggca tgccaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     3841 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     3901 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     3961 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata atcggatcgt
     4021 acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc tgaaacagat
     4081 agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga caatgtatgt
     4141 atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca cgtcgcatcc
     4201 ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt taacgaagca
     4261 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     4321 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
     4381 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
     4441 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
     4501 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
     4561 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
     4621 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
     4681 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
     4741 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
     4801 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
     4861 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
     4921 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
     4981 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
     5041 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
     5101 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
     5161 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
     5221 cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
     5281 aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
     5341 acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
     5401 ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac
     5461 ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt
     5521 tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat
     5581 ttcctttgat attggatcga tccgatgata agctgtcaaa catgagaatt gggtaataac
     5641 tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat
     5701 acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg
     5761 taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata
     5821 ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg
     5881 tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct
     5941 cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca
     6001 atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca tgacaattct
     6061 gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg
     6121 ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt
     6181 ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg
     6241 tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc
     6301 tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct
     6361 aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag
     6421 tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta
     6481 gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct gcatgttttt
     6541 gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg
     6601 cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt
     6661 accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat
     6721 gaattgaaaa gctaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     6781 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     6841 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6901 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6961 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7021 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7081 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7141 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7201 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7261 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7321 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7381 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7441 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7501 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7561 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7621 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7681 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7741 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7801 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7861 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7921 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7981 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8041 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8101 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8161 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8221 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8281 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8341 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8401 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8461 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8521 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8581 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8641 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8701 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8761 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     8821 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     8881 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     8941 atttcacaca ggaaacagct atgaccatga ttacgccaag cttaccgcat caggaaattg
     9001 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     9061 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     9121 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     9181 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     9241 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     9301 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     9361 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     9421 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgc
//
