LOCUS       sequence-270080-        9536 bp    DNA     circular SYN 14-APR-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            TNFRSF9-mTagBFP fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. TNFRSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 9536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF139
FEATURES             Location/Qualifiers
     source          1..9536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        482..1664
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     1612..1632
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1693..1702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2278..2298
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2332..2349
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2356..3057
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(3109..3126)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3115..3339
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3701..4004
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4005..4208
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4159..4179
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4205..4229
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4346..4442
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     4499..4518
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4499..4517
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4528..5550
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    5567..5701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5591..5610)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5645..5664
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5854..5958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5959..6027,6028..6819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6177..6196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6990..7578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7479..7498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7732..7749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8123..8142
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    8141..8196
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     8242..8261
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(8361..9386)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8361..9386)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 cgctaaggcc gagagaaagg cgaagttccg tctacggcta tataccttga aacaggggcg
       61 gcagaagtga cgcaacgcgg ttgcgcggac ggcttcctgt gttttcattg gtcgaagcgt
      121 tctgcgcgtg cgcagagatt ccccagggaa ggcgggccct cttccgaatg tccaatccgg
      181 acattcttcc ggttggacct ccgcggtcag cgctgtgccc ggcgagagat cacgtggggc
      241 gcggaggcgg tgctgctggg gcacggccgt ccagcctcgg cggccatatt tttgaggggc
      301 tgttcatctc gttcacacgc tctgtccgcc atgtttgtga gtggaagcgc cattacccct
      361 tcaagcgact gaaggctgca gggcctctgg tggcccgcat ggggagacca gacccgccag
      421 gcccgccttt ccgcactcag tccgggctta ctttattttg tgagacaggg tctcgcctag
      481 aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      541 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      601 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      661 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      721 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      781 tacttccacg cccctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
      841 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
      901 cctggcttgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
      961 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     1021 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     1081 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     1141 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     1201 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     1261 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     1321 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     1381 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     1441 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     1501 cgatggagtt tccccatact gagtgggtgg agactgaagt taggccagct tggcacttga
     1561 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     1621 agacagtggt tcaaagtttt tttcttccat ttaaggtgtc gtgaaaacta ccccaagctg
     1681 gcctctgcgg ccgccaccat ggtaaacagc tacaatagac tggaggcgat atcaactctt
     1741 gttatactac tctgggtaac tctggtccag ggttcctgtg gcagctgtcc agctggtact
     1801 ttctggaatg gtacagattg tggaaaaaac aaaaatcaag cttgcatccc atgtccccca
     1861 aatagtttct ctagtacagc tggcgaacag aggacatgta acatatgcag gagatgtgaa
     1921 ggtattttta gaacaaagag agaatgtact ccaacaagta atacagagtg tgattgtatg
     1981 tctgggtatc attgttccgg agtaggatgt aataggtgtc aaccagattg caagcaaggt
     2041 caagaacttc taggaaaagg ttgtagagac tgtagctttg gtacctttaa tgatcagaca
     2101 cgggggaact gcaaacgctg gacaaactgc tctttgaatg gactagttgt gcttgtaaat
     2161 ggaaccaata aaagagatgt gatatgtgga tccacccaaa cagttgccac tctggacaca
     2221 atctccatga caattccagc ttctctcgtg cacattgata agaaccatca gcccggggag
     2281 aacctttact tccagggatc cgctggctcc gctgctggtt ctggcgaatt ccatcatcac
     2341 catcaccatg tcgacatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
     2401 gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac
     2461 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     2521 gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc
     2581 atccccgact tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca
     2641 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     2701 atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag
     2761 aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa
     2821 ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag
     2881 accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg
     2941 gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag
     3001 gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct gaactaggct
     3061 agcgtcaggc caagcttggg gatcaattct ctagagctcg ctgatcagcc tcgactgtgc
     3121 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     3181 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     3241 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     3301 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     3361 gctgggccta ctagatagcg gaccccttac cgaaacatcg ccgcattctg cagaggagtc
     3421 gaacttagtg tatgtaaact tctgacccac tggaattgtg atacagtgaa ttataagtga
     3481 aataatctgt ctgtaaacaa ttgttggaaa aatgacttgt gtcatgcaca aagtagatgt
     3541 cctaactgac ttgccaaaac tattgtttgt taacaagaaa tttgtggagt agttgaaaaa
     3601 cgagttttaa tgactccaac ttaagtgtat gtaaacttcc gacttcaact gtatagggat
     3661 ccaattttaa caaaatattc gagcttgcat gcctgcaggt cgttacataa cttacggtaa
     3721 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     3781 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     3841 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     3901 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     3961 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     4021 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     4081 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     4141 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     4201 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     4261 tccatagaag acaccgggac cgatccagcc tccggactct agaggatccg gtactcgagg
     4321 aactgaaaaa ccagaaagtt aactggtaag tttagtcttt ttgtctttta tttcaggtcc
     4381 cggatccggt ggtggtgcaa atcaaagaac tgctcctcag tggatgttgc ctttacttct
     4441 aggcctgtac ggaagtgtta cttctgctct aaaagctgcg gaattgtacc cgcggaatta
     4501 atacgactca ctatagggac tagtaccatg ggaaaatcaa aagaaatcag ccaagacctc
     4561 agaaaaagaa ttgtagacct ccacaagtct ggttcatcct tgggagcaat ttccaaacgc
     4621 ctggcggtac cacgttcatc tgtacaaaca atagtacgca agtataaaca ccatgggacc
     4681 acgcagccgt cataccgctc aggaaggaga cgcgttctgt ctcctagaga tgaacgtact
     4741 ttggtgcgaa aagtgcaaat caatcccaga acaacagcaa aggaccttgt gaagatgctg
     4801 gaggaaacag gtacaaaagt atctatatcc acagtaaaac gagtcctata tcgacataac
     4861 ctgaaaggcc actcagcaag gaagaagcca ctgctccaaa accgacataa gaaagccaga
     4921 ctacggtttg caactgcaca tggggacaaa gatcgtactt tttggagaaa tgtcctctgg
     4981 tctgatgaaa caaaaataga actgtttggc cataatgacc atcgttatgt ttggaggaag
     5041 aagggggagg cttgcaagcc gaagaacacc atcccaaccg tgaagcacgg gggtggcagc
     5101 atcatgttgt gggggtgctt tgctgcagga gggactggtg cacttcacaa aatagatggc
     5161 atcatggacg cggtgcagta tgtggatata ttgaagcaac atctcaagac atcagtcagg
     5221 aagttaaagc ttggtcgcaa atgggtcttc caacacgaca atgaccccaa gcatacttcc
     5281 aaagttgtgg caaaatggct taaggacaac aaagtcaagg tattggagtg gccatcacaa
     5341 agccctgacc tcaatcctat agaaaatttg tgggcagaac tgaaaaagcg tgtgcgagca
     5401 aggaggccta caaacctgac tcagttacac cagctctgtc aggaggaatg ggccaaaatt
     5461 cacccaaatt attgtgggaa gcttgtggaa ggctacccga aacgtttgac ccaagttaaa
     5521 caatttaaag gcaatgctac caaatactag gggccctaac cgcggggatc cagacatgat
     5581 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     5641 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     5701 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     5761 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     5821 accgcatcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5881 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5941 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6001 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6061 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6121 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6181 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6241 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6301 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6361 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6421 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6481 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6541 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6601 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6661 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6721 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6781 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6841 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6901 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6961 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7021 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7081 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7141 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7201 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7261 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7321 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7381 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7441 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7501 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7561 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7621 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7681 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7741 gtgagcgagg aagcggatcc ctatacagtt gaagtcggaa gtttacatac accttagcca
     7801 aatacattta aactcacttt ttcacaattc ctgacattta atcctagtaa aaattccctg
     7861 tcttaggtca gttaggatca ccactttatt ttaagaatgt gaaatatcag aataatagta
     7921 gagagaatga ttcatttcag cttttatttc tttcatcaca ttcccagtgg gtcagaagtt
     7981 tacatacact cgactcctct gcagaatgcg gcgatgtttc ggtaaggggt cccgagtagc
     8041 tagttcatgg cagccagcat atggcatatg ttgccaaact ctaaaccaaa tactcattct
     8101 gatgttttaa atgatttgcc ctcccatatg tccttccgag tgagagacac aaaaaattcc
     8161 aacacactat tgcaatgaaa ataaatttcc tttattagcc agaagtcaga tgctcaaggg
     8221 gcttcatgat gtccccataa tttttggcag agggaaaaag atctcagtgg tatttgtgag
     8281 ccagggcatt ggccacacca gccaccacct tctgataggc agcctgcacc tgaggagtga
     8341 attcacgaca ggccttcgaa ctattccttt gccctcggac gagtgctggg gcgtcggttt
     8401 ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg
     8461 atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc
     8521 gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca
     8581 atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg
     8641 aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg
     8701 acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg
     8761 ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg
     8821 gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg
     8881 gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa
     8941 tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc
     9001 tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg
     9061 ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa
     9121 taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc
     9181 gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc
     9241 cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc
     9301 gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac
     9361 gtcgcggtga gttcaggctt tttcatggtg gcctcaggtg cagaggtttc tacagggaaa
     9421 gagagaaagg ctacagttag tttgctgtga tgatgctaag tgccgaggtc agatgtctat
     9481 ggattgtaca cggaatccca acgcaggaac caaaaaacac tcacccaaaa aaatgg
//
