LOCUS       sequence-270499-        2106 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Protein expression/Streptavidin pull-down.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2106)
  TITLE     Opher Gileadi SGC expression vectors
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TBXTA-c028
FEATURES             Location/Qualifiers
     source          1..2106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..681
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGADHPPK"
     primer_bind     639..661
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             709..729
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2059..2103
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctatg tcccctatac taggttattg gaaaattaag
       61 ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat
      121 ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag
      181 tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc
      241 atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag
      301 atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat
      361 agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa
      421 atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct
      481 gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat
      541 gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag
      601 tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt
      661 ggtgccgacc atcctccaaa atcgagctca ggtgtagatc tgggtaccga gaacctgtac
      721 ttccaatcca tgtccaatag cagccctggc accgaaagcg ccggtaagag cctacagtac
      781 cgtgtggacc atttactgag cgccgtggag aacgaactac aggcaggcag cgaaaaaggt
      841 gacccgaccg aacgcgaact gcgtgtgggt ctggaagaaa gcgaactgtg gctgcgcttt
      901 aaagagctga ccaacgaaat gatcgtgacc aaaaacggcc gccgcatgtt tcctgtgctg
      961 aaagttaacg tgagcggcct ggacccgaac gccatgtaca gcttcctgct ggatttcgtt
     1021 gccgcagata accaccgctg gaagtatgtg aacggcgaat gggtgccggg tggcaaaccg
     1081 gaaccgcagg cacctagctg cgtgtatatc cacccggata gccctaactt cggtgcccat
     1141 tggatgaaag ccccggtgag cttcagcaag gtgaagctga ccaacaaact gaacggcggc
     1201 ggccaaatca tgctgaacag cttacataaa tatgaacctc gcatccacat tgtgcgtgtg
     1261 ggcggcccgc aacgtatgat caccagccat tgcttcccgg agacacagtt cattgccgtt
     1321 accgcctacc agaacgagga aattaccgca ctgaaaatta aatacaaccc ttttgcaaaa
     1381 gccttcttag atgccaaaga gcgcagcgac cacaaagaaa tgatggagga accgggcgac
     1441 agccagcagc ctggttatag ccaatggggc tggctgctgc cgggtaccag tacactgtgc
     1501 ccgccggcaa acccgcatcc tcagtttggt ggcgccctga gcttaccgag cacccacagt
     1561 tgtgatcgct atcctaccct gcgtagccat cgtagcagtc cgtacccgag cccgtatgca
     1621 catcgtaaca acagcccgac atatagcgac aacagcccgg cctgtctgag catgctacag
     1681 agccatgata actggagcag cctgggcatg ccggcacatc cgagcatgct gccggtgagt
     1741 cacaatgcca gcccgccgac cagtagcagc cagtatccta gcctgtggag cgttagtaac
     1801 ggtgcagtga ccccgggtag ccaggcagca gcagttagca atggtctggg cgcccagttt
     1861 tttcgcggca gtccggcaca ttacaccccg ctgacccatc ctgtgagtgc cccgagcagt
     1921 agtggtagcc cgctgtatga aggtgccgca gccgccaccg acattgttga cagccagtat
     1981 gatgccgccg cacagggtcg cctgattgca agctggaccc ctgtgagtcc gccgagcatg
     2041 agcagtaaag gtggatacgg cctgaatgat atctttgaag cgcagaagat tgaatggcat
     2101 gaatga
//
