LOCUS       sequence-270560-       11384 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Contains genes of Type I-C CRISPR-Cas system for inducible 
            expression and chromosomal integration at attTn7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Csorgo B, Leon LM, Chau-Ly IJ, Vasquez-Rifo A, Berry JD, Mahendra C,
            Crawford ED, Lewis JD, Bondy-Denomy J
  TITLE     A compact Cascade-Cas3 system for targeted genome engineering.
  JOURNAL   Nat Methods. 2020 Oct 19. pii: 10.1038/s41592-020-00980-w. doi: 
            10.1038/s41592-020-00980-w.
  PUBMED    33077967
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2020 Oct 19. pii: 10.1038/s41592-020-00980-w. doi: 
            10.1038/s41592-020-00980-w."
COMMENT     Sequence Label: pJW31
FEATURES             Location/Qualifiers
     source          1..11384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..22,23..29)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     42..64
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             complement(777..788)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      5224..5233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     mobile_element  5725..5890
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      complement(6160..6748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6240..6259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6919..7710,7711..7779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7542..7561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7780..7884)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7952..7970
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8008..8030)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8130..8149
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8563..8579
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8564..8580
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      8608..8702
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      8805..8891
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    8937..8984
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(9114..9647)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(9836..9841,9842..9858,9859..9864))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    9905..9952
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(9995..11077)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     11039..11058
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(11078..11155)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
       61 caggaaacag aattcgagct gtgaaaggga gggccgatgg cctacggaat tcgcttaatg
      121 gtctggggcg agcgtgcctg cttcacccgc ccggaaatga aggtggaacg cgtctcttac
      181 gatgcgatca cgccgtccgc cgcgcgcggc attctcgagg ctatccactg gaagccggcg
      241 attcgctggg tggtggatcg cattcaagtg cttaagccga tccgcttcga atccatccgg
      301 cgcaacgagg tcggcggcaa gctgtccgct gtcagcgtcg gtaaggcaat gaaggccggg
      361 cgtactaatg gtctggtgaa tctggtcgag gaggatcgcc agcagcgcgc gactactctg
      421 ctgcgcgatg tctcctatgt catcgaggcg catttcgaga tgactgacag ggctggcgcc
      481 gacgatacgg tgggcaagca tctggatatc ttcaaccgtc gcgcacggaa ggggcagtgc
      541 ttccatacac cctgcctagg cgtgcgcgag tttccggcca gttttcggtt gctggaagag
      601 ggcagtgccg agcctgaagt cgatgccttt ctgcgcggcg agcgtgatct gggctggatg
      661 ctgcatgaca ttgacttcgc cgatggcatg accccgcact tcttccgtgc cctgatgcgc
      721 gatgggctga tcgaggtgcc ggccttcagg gcggcagagg acaaggcatg atcctttcgg
      781 ccctcaatga ctattatcag cgactgctgg agcggggtga agcgaatatc tcacccttcg
      841 gctacagcca agaaaagatc agttacgccc tgctgctgtc cgcacaagga gagttgctgg
      901 acgtgcagga cattcgcttg ctctctggca agaagcctca acccaggctt atgagtgtgc
      961 cgcagccgga gaagcgcacc tcgggcatca agtccaacgt actgtgggac aagaccagct
     1021 atgtgctggg tgttagtgcc aagggcggag agcgtactca gcaggagcac gagtccttca
     1081 agacgctgca ccggcagatc ttggttgggg aaggcgaccc cggtctgcag gccttgctcc
     1141 agttcctcga ctgttggcag ccggagcagt tcaagccccc gctgttcagc gaagcaatgc
     1201 tcgacagcaa cttagtgttc cgcctagacg gccaacaacg ctatctgcac gagactccgg
     1261 cggccctggc gttgcgtacc cggctgttgg ccgacggcga cagccgcgag gggctgtgcc
     1321 tagtctgcgg ccaacgtcag ccgttggcgc gcctgcatcc agcggtcaag ggcgtcaatg
     1381 gtgcccagag ttcgggggct tccatcgtct ccttcaacct cgacgctttt tcctcctacg
     1441 gcaagagcca gggggaaaat gctccggtct ccgaacaggc cgcctttgcc tacaccacgg
     1501 tgctcaacca tttgttgcgt cgcgacgagc acaaccgcca gcgcctgcag attggcgacg
     1561 cgagtgtggt gttctgggcg caggcggata ctcctgctca ggtggccgcc gccgagtcga
     1621 ccttctggaa cctgctggag ccacccgcag atgatggtca ggaagcggaa aagctgcgcg
     1681 gcgtgctgga tgctgtggcc acggggcggc ccttgcatga gctcgactcg ctaatggagg
     1741 aaggcacccg catttttgtg ttagggctgg cgcccaatac ctcgcgactg tccattcggt
     1801 tctgggcagt cgatagcctt gcggtattca cccagcatct ggccgagcat ttccgggata
     1861 tgcaccttga gcctctgccc tggaagacgg agccggccat ctggcgcttg ctctatgcta
     1921 ccgcgcccag tcgtgacggc agagccaaga ccgaagactt actcccacaa ctggccggtg
     1981 aaatgacccg cgccatcctg accggcagcc gctatccgcg cagtttgcta gccaacctga
     2041 tcatgcgcat gcgtgccgac ggcgacgtct ctggcatacg cgtcgcgctg tgcaaggccg
     2101 tgctcgctcg cgaggcacgc ctgagcggca aaattcacca agaggagcta cctatgagtc
     2161 tcgacaagga cgccagcaac cccggctatc gcttggggag gctgttcgcc gtgttggaag
     2221 gcgcccagcg cgcagccctg ggcgacaggg tcaatgccac tatccgtgac cgctactacg
     2281 gtgccgcgtc cagcacgcca gccacggttt tcccgatact gctgcgcaac acacaaaacc
     2341 acttggccaa gctgcgcaag gagaagcccg gactagcagt gaacctagag cgcgatatag
     2401 gcgaaatcat tgacggtatg cagagccaat tcccgcgttg cctgcgcctg gaggaccagg
     2461 gacgctttgc tattggttac taccaacagg cccaggcccg tttcaaccgt ggccccgatt
     2521 ccgtcgagta aggagcagaa gaatgaccgc catctccaac cgctacgagt tcgtttacct
     2581 ctttgatgtc agcaatggca atcccaatgg cgacccggat gctggcaaca tgccgcgtct
     2641 cgatccggaa accaaccagg ggttggtcac tgacgtttgc ctcaagcgca agatccgcaa
     2701 ctacgtcagc ctggagcagg aaagtgcccc cggctatgcc atctatatgc aggaaaaatc
     2761 cgtgctgaat aaccagcaca aacaggccta cgaggcgctc ggtatcgagt cagaggcaaa
     2821 gaaactgccc aaggacgaag ccaaggcgcg cgaactgacc tcttggatgt gcaagaactt
     2881 cttcgatgtg cgtgctttcg gggcggtgat gaccaccgag attaatgccg gccaggtgcg
     2941 tggaccgatc caactggcat tcgccacgtc tatcgacccg gtattgccta tggaggtatc
     3001 catcacccgc atggcggtga ctaacgaaaa ggatttggag aaggaacgca ccatgggacg
     3061 caagcacatc gtgccttacg gcttgtaccg cgcccatggt ttcatctctg ccaagttggc
     3121 cgagcgaacc ggcttttccg acgacgactt ggaactgcta tggcgcgctt tggccaatat
     3181 gttcgaacac gaccgctcgg cggcacgtgg cgagatggca gcgcgcaagt tgatcgtctt
     3241 caagcatgag catgccatgg gcaatgcacc cgcccatgtg ctgttcggca gcgttaaggt
     3301 cgagcgagtc gagggggacg cagttacacc agcacgcggt ttccaggatt accgtgtcag
     3361 catcgatgcg gaagctctgc ctcagggcgt gagcgtgcgc gagtacctct agagacttgg
     3421 cagggagcgc acctatggac gcggaggcta gcgatactca cttttttgct cactccacct
     3481 taaaggcaga tcgcagcgat tggcagcctc tggtcgagca tctacaggct gttgcccgtt
     3541 tggcaggaga gaaggctgcc ttcttcggcg gcggtgaatt agctgctctt gctggtctgt
     3601 tgcatgactt gggtaaatac actgacgagt ttcagcggcg tattgcgggt gatgccatcc
     3661 gtgtcgatca ctctactcgc ggggccatac tggcggtaga acgctatggc gcgctaggtc
     3721 aattgctagc ctacggcatc gctggccacc atgccgggtt ggccaatggc cgcgaggctg
     3781 gtgagcgaac tgccttggtc gaccgcctga aaggggttgg gctgccacgg ttattggagg
     3841 ggtggtgcgt ggaaatcgtg ctacccgagc gccttcaacc accgccacta aaagcgcgcc
     3901 tggaaagagg tttctttcag ttggcctttc ttggccggat gctcttttcc tgcttggttg
     3961 atgcggatta tctagatacc gaagccttct accaccgcgt cgaaggacgg cgctcccttc
     4021 gcgagcaagc gcggccgacc ttggccgagt tacgcgcagc ccttgatcgg catctgactg
     4081 agttcaaggg agatacgccg gtcaaccgcg ttcgcgggga gatattggcc ggcgtgcgcg
     4141 gcaaggcgag cgaacttccc gggctgtttt ctctcacagt gcccacagga ggcggcaaga
     4201 ccctggcctc tctggctttc gccctggatc acgctctagc tcatgggctg cgccgggtga
     4261 tctacgtgat tcccttcact agcatcgtcg agcagaacgc tgcggtattc cgtcgtgcac
     4321 tcggggcctt aggcgaagag gcggtgctgg agcatcacag cgccttcgtt gatgaccgcc
     4381 ggcagagcct ggaggccaag aagaaactga acctagcgat ggagaactgg gacgcgccta
     4441 tcgtggtgac cactgcagtg cagttcttcg aaagcctgtt tgccgaccgt ccagcccagt
     4501 gccgcaagct acacaacatc gccggcagcg tggtgattct tgacgaggca cagaccctac
     4561 cgctcaagct gttgcggccc tgcgttgccg cccttgatga actggcgctc aactaccgtt
     4621 gtagcccagt tctctgtact gccacgcagc cagcgcttca atcgccggat ttcatcggtg
     4681 ggctgcagga cgtacgtgag ctggcgcccg agccgcagcg gctgttccgg gagttggtgc
     4741 gggtacgaat acggacattg ggcccgctcg aagatgcggc cttgactgag cagatcgcca
     4801 ggcgtgaaca agtgctgtgc atcgtcaaca atcgacgcca ggcccgtgcg ctctatgagt
     4861 cgcttgccga gttgcccggt gcccgccatc tcaccaccct gatgtgcgcc aagcaccgta
     4921 gcagcgtgct ggccgaggtg cgccagatgc tcaaaaaggg ggagccctgt cgcctggtgg
     4981 ccacctcgct gatcgaggcc ggtgtggatg tggattttcc cgtggtactg cgtgccgagg
     5041 ctggattgga ttccatcgcc caggccgcgg gacgctgcaa tcgcgaaggc aagcggccgc
     5101 tggccgaaag cgaggtgctg gtgttcgccg cggccaattc tgactgggcg ccacccgagg
     5161 aactcaagca gttcgcccag gccgcccgcg aagtgatgcg cctgcacccg gatgattgcc
     5221 tgtccatggc ggccatcgag cggtattttc gcatactgta ctggcagaag ggcgcggagg
     5281 agttggatgc gggtaacctg ctcggcctga ttgagagagg ccggctcgat ggcctgccct
     5341 acgagacttt ggccaccaag ttccgcatga tcgacagcct tcaactgccg gtgatcatcc
     5401 catttgatga cgaggccaga gcagccctgc gcgagctgga gttcgccgac ggctgcgccg
     5461 ccatcgcccg tcgcctgcag ccatatctgg tgcagatgcc acgcaagggt tatcaggcat
     5521 tgcgggaagc cggtgcgatc caggcggcgg caggtacgcg ttatggtgag cagtttatgg
     5581 cgttggtcaa ccctgatctg tatcaccacc aattcgggtt gcactgggat aatccggcct
     5641 ttgtcagcag cgagcggcta tgttggtagt gcaggaattc ctcgagaagc ttgggcccgg
     5701 tacctcgcga aggccttgca ggccaaccag ataagtgaaa tctagttcca aactattttg
     5761 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     5821 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     5881 tccgcccaca gcggggcatt tttcttcctg ttatgtttgg gcgctgcatt aatgaatcgg
     5941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6001 cccgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     7921 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     7981 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8041 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8101 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8161 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     8221 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     8281 acgccagcta gaggaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat
     8341 ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat
     8401 ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca
     8461 gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc
     8521 tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggg gttcgaggtc gacggtatcg
     8581 ataagctagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag
     8641 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     8701 atccaagcta gactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
     8761 gtgcccttaa acgcctgggg taatgactct ctagcttgag gcatcaaata aaacgaaagg
     8821 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     8881 gtaggacaaa tccgccgcta ggagcttgcg gcccggacga tcgaattggg gatcttgaag
     8941 tacctattcc gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaagc
     9001 tgatgtgctt aaaaacttac tcaatggaat tctagatcga attggccgcg gcgttgtgac
     9061 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     9121 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     9181 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     9241 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc
     9301 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     9361 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     9421 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     9481 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     9541 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     9601 ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca
     9661 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     9721 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     9781 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
     9841 acgaaccgaa caggcttatg tcaattcgat ccattgctgt tgacaaaggg aatcagggga
     9901 tcttgaagtt cctattccga agttcctatt ctctagaaag tataggaact tcagagcgct
     9961 tttgaagcta attcgatcat gcattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    10021 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    10081 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
    10141 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
    10201 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
    10261 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
    10321 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt aggaacgatg ccctcattca
    10381 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
    10441 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
    10501 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
    10561 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
    10621 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
    10681 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
    10741 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
    10801 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
    10861 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
    10921 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
    10981 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
    11041 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
    11101 cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt
    11161 aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc gactgcacgg
    11221 tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
    11281 taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg
    11341 cgccgacatc ataacggttc tggcaaatat tctgaaatga gctg
//
