LOCUS       sequence-270718-       16303 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor plasmid of targeted inducible-dCAS9-P300core knockin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16303)
  TITLE     Lian Lab CRISPR plasmids
REFERENCE   2  (bases 1 to 16303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYJ28-AAVS1-XLone-dCas9-p300core
FEATURES             Location/Qualifiers
     source          1..16303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     339..358
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    357..412
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     458..477
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    587..1063
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     845..864
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     misc_feature    1095..1683
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1201..1212
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1610..1630
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1714..2460)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(2461..2484)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2484..2493
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2513..2724)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     promoter        2766..3133
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    2774..2792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2810..2828
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2846..2864
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2882..2900
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2918..2936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2954..2972
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2990..3008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      3140..3149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3146..3172,3173..3175,3176..5662,5663..5665,
                     5666..7249)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3146..7249
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7250..7297
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7304..9154
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      8918..8927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9161..9184
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    9368..9502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9446..9465
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    9527..9751
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9740..9757
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(9789..10388)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9872..9892)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     10369..10388
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(10398..10451)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    10475..10500
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    10507..11310
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(11349..11367)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11388..11404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11412..11428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11436..11442,11443..11460,11461..11466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11481..11502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11619..11636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11790..12378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11870..11889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(12502..12570,12571..13362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12720..12739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(13723..13742)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     14337..14356
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(14759..15061)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(14861..14880)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     15184..15206
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15198..15215
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15199..15215
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15222..15241
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15222..15240
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    15275..16111
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    16185..16206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16221..16226,16227..16244,16245..16251)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16259..16275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16283..16299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 attacgccaa gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg
       61 actgggagta gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt
      121 agggatgtta aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc
      181 aaggcttttc tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct
      241 ctttatagcc aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa
      301 accaaatact cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag
      361 agacacaaaa aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa
      421 gtcagatgct caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct
      481 cagtggtatt tgtgagccag ggcattggcc acaccagcca ccaccttctg ataggcagcc
      541 tgcacctgag gagtgaattc acgcgcctgg ttacctacaa aatcagaagg acagggaagg
      601 gagcagtggt tcacgcctgt aatcccagca atttgggagg ccaaggtggg tagatcacct
      661 gagattagga gttggagacc agcctggcca atatggtgaa accccgtctc taccaaaaaa
      721 acaaaaatta gctgagcctg gtcatgcatg cctggaatcc caacaactcg ggaggctgag
      781 gcaggagaat cgcttgaacc caggaggcgg agattgcagt gagccaagat tgtgccactg
      841 cactccagct tggttcccaa tagaccccgc aggccctaca ggttgtcttc ccaacttgcc
      901 ccttgctcca taccaccccc ctccacccca taatattata gaaggacacc tagtcagaca
      961 aaatgatgca acttaatttt attaggacaa ggctggtggg cactggagtg gcaacttcca
     1021 gggccaggag aggcactggg gaggggtcac agggatgcca cccgtagatc tctcgagcag
     1081 cgctcggtat cgatgcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     1141 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     1201 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacata
     1261 ggcgagcagc caaggaaagg acgatgattt ccccgacaac accacggaat tgtcagtgcc
     1321 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     1381 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     1441 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     1501 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     1561 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     1621 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     1681 attatcgata agcttgatat cgaattcacg cgtttacccg gggagcatgt caaggtcaaa
     1741 atcgtcaaga gcgtcagcag gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg
     1801 gagcatgtct aagtcaaaat cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag
     1861 ctgtttctcc aggccacata tgattagttc caggccgaaa aggaaggcag gttcggctcc
     1921 ctgccggtcg aacagctcaa ttgcttgttt cagaagtggg ggcatagaat cggtggtagg
     1981 tgtctctctt tcctcttttg ctacttgatg ctcctgttcc tccaatacgc agcccagtgt
     2041 aaagtggccc acggcggaca gagcgtacag tgcgttctcc agggagaagc cttgctgaca
     2101 caggaacgcg agctgatttt ccagggtttc gtactgtttc tctgttgggc gggtgccgag
     2161 atgcacttta gccccgtcgc gatgtgagag gagagcacag cggtatgact tggcgttgtt
     2221 ccgcagaaag tcttgccatg actcgccttc cagggggcag gagtgggtat gatgcctgtc
     2281 cagcatctcg attggcaggg catcgagcag ggcccgcttg ttcttcacgt gccagtacag
     2341 ggtaggctgc tcaactccca gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc
     2401 gactccattg agtaattcca gagcagagtt tatgactttg ctcttgtcca gtctagacat
     2461 cttatcgtca tcgtctttgt aatccatggt ggcggatccc gcgtcacgac acctgtgttc
     2521 tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt atatacggtt
     2581 ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc cagtttaccc
     2641 cgcgccacct tctctaggca ccggttcaat tgccgacccc tccccccaac ttctcgggga
     2701 ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gccactcgag tggaattatc
     2761 acctcgagtt tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt
     2821 gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta
     2881 ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt
     2941 atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt
     3001 gatagagaac gtataagctt tgcttatgta aaccagggcg cctataaaag agtgctgatt
     3061 ttttgagtaa acttcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc
     3121 ctaccctcgt aaaggtaccg ccaccatgga caagaagtac agcatcggcc tggccatcgg
     3181 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
     3241 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
     3301 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
     3361 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
     3421 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
     3481 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
     3541 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
     3601 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
     3661 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     3721 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
     3781 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
     3841 gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt
     3901 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
     3961 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
     4021 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
     4081 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
     4141 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
     4201 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt
     4261 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
     4321 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
     4381 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt
     4441 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt
     4501 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac
     4561 catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat
     4621 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag
     4681 cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga
     4741 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
     4801 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
     4861 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
     4921 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
     4981 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     5041 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     5101 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     5161 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     5221 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     5281 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     5341 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     5401 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     5461 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     5521 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     5581 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     5641 gctgtccgac tacgatgtgg acgccatcgt gcctcagagc tttctgaagg acgactccat
     5701 cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc
     5761 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
     5821 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     5881 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     5941 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
     6001 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     6061 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     6121 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     6181 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     6241 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
     6301 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
     6361 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
     6421 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
     6481 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
     6541 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
     6601 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
     6661 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
     6721 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
     6781 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
     6841 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
     6901 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca
     6961 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc
     7021 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct
     7081 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
     7141 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
     7201 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca agcgacctgc
     7261 cgccacaaag aaggctggac aggctaagaa gaagaaagga tccattttca aaccagaaga
     7321 actacgacag gcactgatgc caactttgga ggcactttac cgtcaggatc cagaatccct
     7381 tccctttcgt caacctgtgg accctcagct tttaggaatc cctgattact ttgatattgt
     7441 gaagagcccc atggatcttt ctaccattaa gaggaagtta gacactggac agtatcagga
     7501 gccctggcag tatgtcgatg atatttggct tatgttcaat aatgcctggt tatataaccg
     7561 gaaaacatca cgggtataca aatactgctc caagctctct gaggtctttg aacaagaaat
     7621 tgacccagtg atgcaaagcc ttggatactg ttgtggcaga aagttggagt tctctccaca
     7681 gacactgtgt tgctacggca aacagttgtg cacaatacct cgtgatgcca cttattacag
     7741 ttaccagaac aggtatcatt tctgtgagaa gtgtttcaat gagatccaag gggagagcgt
     7801 ttctttgggg gatgaccctt cccagcctca aactacaata aataaagaac aattttccaa
     7861 gagaaaaaat gacacactgg atcctgaact gtttgttgaa tgtacagagt gcggaagaaa
     7921 gatgcatcag atctgtgtcc ttcaccatga gatcatctgg cctgctggat tcgtctgtga
     7981 tggctgttta aagaaaagtg cacgaactag gaaagaaaat aagttttctg ctaaaaggtt
     8041 gccatctacc agacttggca cctttctaga gaatcgtgtg aatgactttc tgaggcgaca
     8101 gaatcaccct gagtcaggag aggtcactgt tagagtagtt catgcttctg acaaaaccgt
     8161 ggaagtaaaa ccaggcatga aagcaaggtt tgtggacagt ggagagatgg cagaatcctt
     8221 tccataccga accaaagccc tctttgcctt tgaagaaatt gatggtgttg acctgtgctt
     8281 ctttggcatg catgttcaag agtatggctc tgactgccct ccacccaacc agaggagagt
     8341 atacatatct tacctcgata gtgttcattt cttccgtcct aaatgcttga ggactgcagt
     8401 ctatcatgaa atcctaattg gatatttaga atatgtcaag aaattaggtt acacaacagg
     8461 gcatatttgg gcatgtccac caagtgaggg agatgattat atcttccatt gccatcctcc
     8521 tgaccagaag atacccaagc ccaagcgact gcaggaatgg tacaaaaaaa tgcttgacaa
     8581 ggctgtatca gagcgtattg tccatgacta caaggatatt tttaaacaag ctactgaaga
     8641 tagattaaca agtgcaaagg aattgcctta tttcgagggt gatttctggc ccaatgttct
     8701 ggaagaaagc attaaggaac tggaacagga ggaagaagag agaaaacgag aggaaaacac
     8761 cagcaatgaa agcacagatg tgaccaaggg agacagcaaa aatgctaaaa agaagaataa
     8821 taagaaaacc agcaaaaata agagcagcct gagtaggggc aacaagaaga aacccgggat
     8881 gcccaatgta tctaacgacc tctcacagaa actatatgcc accatggaga agcataaaga
     8941 ggtcttcttt gtgatccgcc tcattgctgg ccctgctgcc aactccctgc ctcccattgt
     9001 tgatcctgat cctctcatcc cctgcgatct gatggatggt cgggatgcgt ttctcacgct
     9061 ggcaagggac aagcacctgg agttctcttc actccgaaga gcccagtggt ccaccatgtg
     9121 catgctggtg gagctgcaca cgcagagcca ggacggatcc gattacaaag acgatgacga
     9181 taagtagact agtagaccac ctcccctgcg agctaagctg gacagccaat gacgggtaag
     9241 agagtgacat ttttcactaa cctaagacag gagggccgtc agagctactg cctaatccaa
     9301 agacgggtaa aagtgataaa aatgtatcac tccaacctaa gacaggcgca gcttccgagg
     9361 gatttgagat ccagacatga taagatacat tgatgagttt ggacaaacca aaactagaat
     9421 gcagtgaaaa aaatgcctta tttgtgaaat ttgtgatgct attgccttat ttgtaaccat
     9481 tataagctgc aataaacaag ttctagtcaa taatcaatgt cgagacccat agagcccacc
     9541 gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc
     9601 ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat
     9661 taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa
     9721 acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc
     9781 cctctagatc aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc
     9841 tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg
     9901 gaggtctcca ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg
     9961 acggcgctgc ccagaccctt gccctggtgg tcgggcgaga cgccgacggt ggccaggaac
    10021 cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc
    10081 agccgggaac cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct
    10141 tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc
    10201 ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg
    10261 atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg
    10321 agggtgcgta cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac
    10381 tcggtcatct cgagcctagg gccgggattc tcctccacgt caccgcatgt tagaagactt
    10441 cctctgccct ctccgctgcc agatctctcg aggccctgtg ggaggaagag aagaggtcag
    10501 aagcttgccc cactgtgggg tggaggggac agataaaagt acccagaacc agagccacat
    10561 taaccggccc tgggaatata aggtggtccc agctcgggga cacaggatcc ctggaggcag
    10621 caaacatgct gtcctgaagt ggacataggg gcccgggttg gaggaagaag actagctgag
    10681 ctctcggacc cctggaagat gccatgacag ggggctggaa gagctagcac agactagaga
    10741 ggtaaggggg gtaggggagc tgcccaaatg aaaggagtga gaggtgaccc gaatccacag
    10801 gagaacgggg tgtccaggca aagaaagcaa gaggatggag aggtggctaa agccagggag
    10861 acggggtact ttggggttgt ccagaaaaac ggtgatgatg caggcctaca agaaggggag
    10921 gcgggacgca agggagacat ccgtcggaga aggccatcct aagaaacgag agatggcaca
    10981 ggccccagaa ggagaaggaa aagggaaccc agcgagtgaa gacggcatgg ggttgggtga
    11041 gggaggagag atgcccggag aggacccaga cacggggagg atccgctcag aggacatcac
    11101 gtggtgcagc gccgagaagg aagtgctccg gaaagagcat ccttgggcag caacacagca
    11161 gagagcaagg ggaagaggga gtggaggaag acggaacctg aaggaggcgg cagggaagga
    11221 tctgggccag ccgtagaggt gacccaggcc acaagctgca gacagaaagc ggcacaggcc
    11281 caggggagag aatgctggtc agagaaagca aagggcgaat tcgtttaaac ctgcaggact
    11341 agctagtccc tttagtgagg gttaattctg agcttggcgt aatcatggtc atagctgttt
    11401 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    11461 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    11521 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    11581 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    11641 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    11701 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    11761 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    11821 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    11881 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    11941 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    12001 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    12061 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    12121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    12181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    12241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    12301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    12361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    12421 aacgaaaact cacgttaagg gattttggtc atgagacaat aaccctgata aatgcttcaa
    12481 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    12541 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    12601 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    12661 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    12721 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    12781 cactattctc agaatgactt ggttgagtat tcaccagtca cagaaaagca tcttacggat
    12841 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    12901 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    12961 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    13021 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    13081 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    13141 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    13201 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    13261 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    13321 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    13381 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    13441 atcctttttg ataatctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    13501 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca
    13561 cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaataa ttcagaagaa
    13621 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
    13681 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
    13741 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
    13801 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgcatgg gtcacgacga
    13861 gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc
    13921 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
    13981 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
    14041 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
    14101 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
    14161 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
    14221 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
    14281 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
    14341 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
    14401 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agagcttgat cccctgcgcc
    14461 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
    14521 cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta
    14581 gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc
    14641 ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg
    14701 ctttctacgt gaaaaggatc taggtgaaga tcctttttga taatctcatg cctgacattt
    14761 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
    14821 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
    14881 gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca
    14941 gcagacgtgc actggccagg gggatcacca tccgtcgccc cggcgtgtca ataatatcac
    15001 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
    15061 gccgtacgta taggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    15121 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    15181 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
    15241 gcgaattgaa tttagcggcc gcgaattcgc ccttagagca gagccaggaa cccctgtagg
    15301 gaaggggcag gagagccagg ggcatgagat ggtggacgag gaagggggac agggaagcct
    15361 gagcgcctct cctgggcttg ccaaggactc aaacccagaa gcccagagca gggccttagg
    15421 gaagcgggac cctgctctgg gcggaggaat atgtcccaga tagcactggg gactctttaa
    15481 ggaaagaagg atggagaaag agaaagggag tagaggcggc cacgacctgg tgaacaccta
    15541 ggacgcacca ttctcacaaa gggagttttc cacacggaca cccccctcct caccacagcc
    15601 ctgccaggac ggggctggct actggcctta tctcacaggt aaaactgacg cacggaggaa
    15661 caatataaat tggggactag aaaggtgaag agccaaagtt agaactcagg accaacttat
    15721 tctgattttg tttttccaaa ctgcttctcc tcttgggaag tgtaaggaag ctgcagcacc
    15781 aggatcagtg aaacgcacca gacagccgcg tcagagcagc tcaggttctg ggagagggta
    15841 gcgcagggtg gccactgaga accgggcagg tcacgcatcc cccccttccc tcccaccccc
    15901 tgccaagctc tccctcccag gatcctctct ggctccatcg taagcaaacc ttagaggttc
    15961 tggcaaggag agagatggct ccaggaaatg ggggtgtgtc accagataag gaatctgcct
    16021 aacaggaggt gggggttaga cccaatatca ggagactagg aaggaggagg cctaaggatg
    16081 gggcttttct gtcaccaatc ctgtccctag taaagcttag tactgtcgat ccgctggcac
    16141 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    16201 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    16261 gtgagcggat aacaatttca cacaggaaac agctatgacc atg
//
