LOCUS       sequence-271659-        9586 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of gRNA against human PLOD2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Sato K, Parag-Sharma K, Terajima M, Musicant AM, Murphy RM, Ramsey 
            MR, Hibi H, Yamauchi M, Amelio AL
  TITLE     Lysyl hydroxylase 2-induced collagen cross-link switching promotes 
            metastasis in head and neck squamous cell carcinomas.
  JOURNAL   Neoplasia. 2021 Jun 6;23(6):594-606. doi: 10.1016/j.neo.2021.05.014.
  PUBMED    34107376
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neo.2021.05"; journalName: "Neoplasia"; date: "2021-06-6-
            6"; volume: "23"; issue: "6"; pages: "594-606"
COMMENT     Sequence Label: pSico_U6-PLOD2 sgRNA4
FEATURES             Location/Qualifiers
     source          1..9586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..582
                     /label=chicken beta-actin promoter
     intron          583..1599
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1523..1545
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1607..1626
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1664..1684
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1688..1708
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1712..1732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1748..2443
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             2444..2497
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(2941..2960)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3195..3228)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3284..3872
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3337..3357)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3755..3766)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3875..3891)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3876..3892)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4401..4581
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4643..5231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4723..4742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5402..6193,6194..6262))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6025..6044
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6263..6367)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6442..6461)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6633..7012
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7014..7212
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7167..7187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7230..7410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7457..7582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8079..8312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8497..8541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8839..8956
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(9209..9242)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     9246..9266
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      361 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      421 ggggggsgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
      481 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      541 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      601 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      661 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
      721 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
      781 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
      841 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
      901 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
      961 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
     1021 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
     1081 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
     1141 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
     1201 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
     1261 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
     1321 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     1381 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     1441 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     1501 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1561 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1621 attgtgctgt ctcatcattt tggcaaagaa ttccgccacc atgccaaaaa agaagagaaa
     1681 ggtagatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg tacacgtgtc
     1741 aacgcgtatg gtgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     1801 caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     1861 cacccagacc atgagaatca aggcggtcga gggcggccct ctccccttcg ccttcgacat
     1921 cctggctacc agcttcatgt acggcagcaa aaccttcatc aaccacaccc agggcatccc
     1981 cgacttcttt aagcagtcct tccccgaggg cttcacatgg gagagagtca ccacatacga
     2041 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     2101 caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac
     2161 actcggctgg gaggcctcca ccgagaccct gtaccccgct gacggcggcc tggaaggcag
     2221 agccgacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaact tgaagaccac
     2281 atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacag
     2341 aagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc
     2401 tgtggccaga tactgcgacc tccctagcaa actggggcac agagagggca gaggaagtct
     2461 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg aagaccttca acatctccca
     2521 gcaggatcta gaattagtag aagtagcgac agagaagatt acaatgcttt atgaggataa
     2581 taaacatcat gtgggagcgg caattcgtac gaaaacagga gaaatcattt cggcagtaca
     2641 tattgaagcg tatataggac gagtaactgt ttgtgcagaa gccattgcga ttggtagtgc
     2701 agtttcgaat ggacaaaagg attttgacac gattgtagct gttagacacc cttattctga
     2761 cgaagtagat agaagtattc gagtggtaag tccttgtggt atgtgtaggg agttgatttc
     2821 agactatgca ccagattgtt ttgtgttaat agaaatgaat ggcaagttag tcaaaactac
     2881 gattgaagaa ctcattccac tcaaatatac ccgaaattaa tcgacatgat aagatacatt
     2941 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3001 tgtgatgcta ttgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3061 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcaga
     3121 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt agatcgaatt
     3181 cgtcgaggga cctaataact tcgtatagca tacattatac gaagttatac atgtttaagg
     3241 gttccggttc cactaggtac aattcgatat caagcttatc gataatcaac ctctggatta
     3301 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3361 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3421 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3481 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3541 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3601 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3661 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3721 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3781 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3841 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc
     3901 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     3961 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     4021 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4081 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     4141 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     4201 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     4261 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     4321 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     4381 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4441 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4501 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4561 cagtgtggaa aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5041 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5221 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5281 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5341 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5401 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5461 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5521 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5581 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5641 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5701 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5761 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5821 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5881 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5941 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6001 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6061 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6121 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6181 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6241 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6301 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6361 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     6421 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     6481 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     6541 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     6601 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     6661 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     6721 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     6781 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     6841 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     6901 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     6961 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     7021 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     7081 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     7141 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     7201 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     7261 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7321 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7381 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     7441 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     7501 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     7561 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     7621 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     7681 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     7741 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     7801 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     7861 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     7921 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     7981 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8041 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8101 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8161 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     8221 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     8281 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     8341 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8401 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     8461 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     8521 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     8581 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     8641 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     8701 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     8761 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     8821 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     8881 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     8941 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc
     9001 gggcccgctc tagagatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacgga
     9061 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag
     9121 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct
     9181 acattttaca tgataggctt ggatttctat aacttcgtat agcatacatt atacgaagtt
     9241 ataaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat
     9301 aagtatccct tggagaacca ccttgttggt gagcaaacag tccagacgtg tttaagagct
     9361 atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg
     9421 gcaccgagtc ggtgcttttt ttctcgagta ctaggatcca ttaggcggcc gcgtggataa
     9481 ccgtattacc gccatgcatc ccgggtgact gactgagcgg ccgcactagt tattaatagt
     9541 aatcaattac ggggtcatta gttcatagcc catatatgga gttccg
//
