LOCUS       sequence-271771-       12072 bp    DNA     circular SYN 15-APR-2025
DEFINITION  3rd generation lentiviral expression of untagged Casilio-ME2.1 
            effector PUFa-hGADD45A-hTET1(CD) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12072)
  AUTHORS   Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li
            S, Cheng AW
  TITLE     Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated 
            RNA-guided coupling of methylcytosine oxidation and DNA repair 
            pathways.
  JOURNAL   Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
  PUBMED    31541098
REFERENCE   2  (bases 1 to 12072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-20"; volume: "10(1):4296. doi"; pages: " 
            10.1038/s41467-019-12339-7"
COMMENT     Sequence Label: pAT360_CMV/CAG_PUFa-hGADD45A-hTET1(CD)
FEATURES             Location/Qualifiers
     source          1..12072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3289..3888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3289..3308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3785..3805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3930..4047
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4164..4467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4468..4671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4622..4642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4694..4718
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4769..4789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4793..4813
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4817..4837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4928..4948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6005..6025
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6029..6049
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(8873..8897)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8899..8940
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8982..9570
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9035..9055)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9453..9464)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9642..9875
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9953..10074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9990..10009)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10044..10063
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10114..10249
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10269..10288)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10270..10288)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10298..10315)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10298..10314)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10307..10329)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10456..10911
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10543..10562)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10753..10774
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10937..11041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11042..11110,11111..11902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11260..11279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccgg agcttaccat gaccgagtac
     3301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3481 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     3541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attctagatc
     3901 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     3961 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4021 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     4081 tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt
     4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4441 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4501 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4561 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4621 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     4681 actgtcggga tcaacaagtt tgtacaaaaa agcaggctcc gaattcaccg gtgccgccac
     4741 catgatcgat ggcggaggcg gatctgatcc aaaaaagaag agaaaggtag atccaaaaaa
     4801 gaagagaaag gtagatccaa aaaagaagag aaaggtagga tctaccggat ctagaaacga
     4861 tggtggtggt ggaagcgggg gtgggggcag cggtggaggg ggaagcgggc gcgccgggat
     4921 cctccccccc aagaaaaaga ggaaggtatc tagaggccgc agccgccttt tggaagattt
     4981 tcgaaacaac cggtacccca atttacaact gcgggagatt gctggacata taatggaatt
     5041 ttcccaagac cagcatgggt ccagattcat tcagctgaaa ctggagcgtg ccacaccagc
     5101 tgagcgccag cttgtcttca atgaaatcct ccaggctgcc taccaactca tggtggatgt
     5161 gtttggtaat tacgtcattc agaagttctt tgaatttggc agtcttgaac agaagctggc
     5221 tttggcagaa cggattcgag gccacgtcct gtcattggca ctacagatgt atggcagccg
     5281 tgttatcgag aaagctcttg agtttattcc ttcagaccag cagaatgaga tggttcggga
     5341 actagatggc catgtcttga agtgtgtgaa agatcagaat ggcaatcacg tggttcagaa
     5401 atgcattgaa tgtgtacagc cccagtcttt gcaatttatc atcgacgcgt ttaagggaca
     5461 ggtatttgcc ttatccacac atccttatgg ctgccgagtg attcagagaa tcctggagca
     5521 ctgtctccct gaccagacac tccctatttt agaggagctt caccagcaca cagagcagct
     5581 ggtacaggat caatatggaa attatgtaat ccaacatgta ctggagcacg gtcgtcctga
     5641 ggataaaagc aaaattgtag cagaaatccg aggcaatgta cttgtattga gtcagcacaa
     5701 atttgcaagc aatgttgtgg agaagtgtgt tactcacgcc tcacgtacgg agcgcgctgt
     5761 gctcatcgac gaggtgtgca ccatgaacga cggtccccac agtgccttat acaccatgat
     5821 gaaggaccag tatgccaact acgtggtcca gaagatgatt gacgtggcgg agccaggcca
     5881 gcggaagatc gtcatgcata agatccggcc ccacatcgca actcttcgta agtacaccta
     5941 tggcaagcac attctggcca agctggagaa gtactacatg aagaacggtg ttgacttagg
     6001 ggacccaaag aagaagcgca aagtggatcc taaaaagaaa agaaaggtag gcggccgcgg
     6061 cggaggttct ggtggaggat caggtggagg gagtggtgga ggaagtgggg gaggttctgg
     6121 aggtggatcg ttgactttgg aggaattctc ggctggagag cagaagaccg aaaggatgga
     6181 taaggtgggg gatgccctgg aggaagtgct cagcaaagcc ctgagtcagc gcacgatcac
     6241 tgtcggggtg tacgaagcgg ccaagctgct caacgtcgac cccgataacg tggtgttgtg
     6301 cctgctggcg gcggacgagg acgacgacag agatgtggct ctgcagatcc acttcaccct
     6361 gatccaggcg ttttgctgcg agaacgacat caacatcctg cgcgtcagca acccgggccg
     6421 gctggcggag ctcctgctct tggagaccga cgctggcccc gcggcgagcg agggcgccga
     6481 gcagcccccg gacctgcact gcgtgctggt gacgaatcca cattcatctc aatggaagga
     6541 tcctgcctta agtcaactta tttgtttttg ccgggaaagt cgctacatgg atcaatgggt
     6601 tccagtgatt aatctccctg aacggtctag aggccgcggg ggaggcggtt ccggtggcgg
     6661 cggaagcgga ggtggaggat cagggccggc cgaactgccc acctgcagct gtcttgatcg
     6721 agttatacaa aaagacaaag gcccatatta tacacacctt ggggcaggac caagtgttgc
     6781 tgctgtcagg gaaatcatgg agaataggta tggtcaaaaa ggaaacgcaa taaggataga
     6841 aatagtagtg tacaccggta aagaagggaa aagctctcat gggtgtccaa ttgctaagtg
     6901 ggttttaaga agaagcagtg atgaagaaaa agttctttgt ttggtccggc agcgtacagg
     6961 ccaccactgt ccaactgctg tgatggtggt gctcatcatg gtgtgggatg gcatccctct
     7021 tccaatggcc gaccggctat acacagagct cacagagaat ctaaagtcat acaatgggca
     7081 ccctaccgac agaagatgca ccctcaatga aaatcgtacc tgtacatgtc aaggaattga
     7141 tccagagact tgtggagctt cattctcttt tggctgttca tggagtatgt actttaatgg
     7201 ctgtaagttt ggtagaagcc caagccccag aagatttaga attgatccaa gctctccctt
     7261 acatgaaaaa aaccttgaag ataacttaca gagtttggct acacgattag ctccaattta
     7321 taagcagtat gctccagtag cttaccaaaa tcaggtggaa tatgaaaatg ttgcccgaga
     7381 atgtcggctt ggcagcaagg aaggtcgtcc cttctctggg gtcactgctt gcctggactt
     7441 ctgtgctcat ccccacaggg acattcacaa catgaataat ggaagcactg tggtttgtac
     7501 cttaactcga gaagataacc gctctttggg tgttattcct caagatgagc agctccatgt
     7561 gctacctctt tataagcttt cagacacaga tgagtttggc tccaaggaag gaatggaagc
     7621 caagatcaaa tctggggcca tcgaggtcct ggcaccccgc cgcaaaaaaa gaacgtgttt
     7681 cactcagcct gttccccgtt ctggaaagaa gagggctgcg atgatgacag aggttcttgc
     7741 acataagata agggcagtgg aaaagaaacc tattccccga atcaagcgga agaataactc
     7801 aacaacaaca aacaacagta agccttcgtc actgccaacc ttagggagta acactgagac
     7861 cgtgcaacct gaagtaaaaa gtgaaaccga accccatttt atcttaaaaa gttcagacaa
     7921 cactaaaact tattcgctga tgccatccgc tcctcaccca gtgaaagagg catctccagg
     7981 cttctcctgg tccccgaaga ctgcttcagc cacaccagct ccactgaaga atgacgcaac
     8041 agcctcatgc gggttttcag aaagaagcag cactccccac tgtacgatgc cttcgggaag
     8101 actcagtggt gccaatgcag ctgctgctga tggccctggc atttcacagc ttggcgaagt
     8161 ggctcctctc cccaccctgt ctgctcctgt gatggagccc ctcattaatt ctgagccttc
     8221 cactggtgtg actgagccgc taacgcctca tcagccaaac caccagccct ccttcctcac
     8281 ctctcctcaa gaccttgcct cttctccaat ggaagaagat gagcagcatt ctgaagcaga
     8341 tgagcctcca tcagacgaac ccctatctga tgaccccctg tcacctgctg aggagaaatt
     8401 gccccacatt gatgagtatt ggtcagacag tgagcacatc tttttggatg caaatattgg
     8461 tggggtggcc atcgcacctg ctcacggctc ggttttgatt gagtgtgccc ggcgagagct
     8521 gcacgctacc actcctgttg agcaccccaa ccgtaatcat ccaacccgcc tctcccttgt
     8581 cttttaccag cacaaaaacc taaataagcc ccaacatggt tttgaactaa acaagattaa
     8641 gtttgaggct aaagaagcta agaataagaa aatgaaggcc tcagagcaaa aagaccaggc
     8701 agctaatgaa ggtccagaac agtcctctga agtaaatgaa ttgaaccaaa ttccttctca
     8761 taaagcatta acattaaccc atgacaatgt tgtcaccgtg tccccttatg ctctcacaca
     8821 cgttgcgggg ccctataacc attgggtcat cgattgatta attaagaatt cgacccagct
     8881 ttcttgtaca aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     8941 tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggattaca
     9001 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     9061 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     9121 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     9181 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     9241 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     9301 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     9361 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     9421 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     9481 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     9541 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     9601 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     9661 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9721 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9781 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9841 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
     9901 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     9961 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10021 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10081 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    10141 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    10201 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
    10261 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
    10321 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    10381 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    10441 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
    10501 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
    10561 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
    10621 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
    10681 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
    10741 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
    10801 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
    10861 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
    10921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    10981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    11041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    11101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    11161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    11221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    11281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    11341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    11401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    11461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    11521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    11581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    11641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    11701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    11761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    11821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    11881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    11941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    12001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    12061 caaaggatct tc
//
