LOCUS       sequence-271817-        9855 bp    DNA     circular SYN 15-APR-2025
DEFINITION  3rd generation lentiviral expression of Casilio-ME2.4 effector 
            hGADD45A-PUFc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li
            S, Cheng AW
  TITLE     Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated 
            RNA-guided coupling of methylcytosine oxidation and DNA repair 
            pathways.
  JOURNAL   Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
  PUBMED    31541098
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-20"; volume: "10(1):4296. doi"; pages: " 
            10.1038/s41467-019-12339-7"
COMMENT     Sequence Label: pAT356-CMV/CAG_hGADD45A-PUFc
FEATURES             Location/Qualifiers
     source          1..9855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3289..3888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3289..3308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3785..3805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3930..4047
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4164..4467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4468..4671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4622..4642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4694..4718
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5243..5269
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5294..5314
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5318..5338
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5342..5362
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5453..5473
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6530..6550
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6554..6574
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6656..6680)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6682..6723
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6765..7353
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6818..6838)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7236..7247)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7425..7658
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7736..7857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7773..7792)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7827..7846
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7897..8032
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8052..8071)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8053..8071)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8081..8098)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8081..8097)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8090..8112)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8239..8694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8326..8345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8536..8557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8720..8824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8825..8893,8894..9685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9043..9062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccgg agcttaccat gaccgagtac
     3301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3481 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     3541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attctagatc
     3901 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     3961 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4021 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     4081 tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt
     4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4441 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4501 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4561 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4621 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     4681 actgtcggga tcaacaagtt tgtacaaaaa agcaggctcc gaattcaccg gtgccgccac
     4741 catgactttg gaggaattct cggctggaga gcagaagacc gaaaggatgg ataaggtggg
     4801 ggatgccctg gaggaagtgc tcagcaaagc cctgagtcag cgcacgatca ctgtcggggt
     4861 gtacgaagcg gccaagctgc tcaacgtcga ccccgataac gtggtgttgt gcctgctggc
     4921 ggcggacgag gacgacgaca gagatgtggc tctgcagatc cacttcaccc tgatccaggc
     4981 gttttgctgc gagaacgaca tcaacatcct gcgcgtcagc aacccgggcc ggctggcgga
     5041 gctcctgctc ttggagaccg acgctggccc cgcggcgagc gagggcgccg agcagccccc
     5101 ggacctgcac tgcgtgctgg tgacgaatcc acattcatct caatggaagg atcctgcctt
     5161 aagtcaactt atttgttttt gccgggaaag tcgctacatg gatcaatggg ttccagtgat
     5221 taatctccct gaacggtcta gataccctta tgatgtgcca gattatgcca tcgatggcgg
     5281 aggcggatct gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtaga
     5341 tccaaaaaag aagagaaagg taggatctac cggatctaga aacgatggtg gtggtggaag
     5401 cgggggtggg ggcagcggtg gagggggaag cgggcgcgcc gggatcctcc cccccaagaa
     5461 aaagaggaag gtatctagag gccgcagccg ccttttggaa gattttcgaa acaaccggta
     5521 ccccaattta caactgcggg agattgctgg acatataatg gaattttccc aagaccagca
     5581 tgggtccaga ttcattcagc tgaaactgga gcgtgccaca ccagctgagc gccagcttgt
     5641 cttcaatgaa atcctccagg ctgcctacca actcatggtg gatgtgtttg gtaattacgt
     5701 cattcagaag ttctttgaat ttggcagtct tgaacagaag ctggctttgg cagaacggat
     5761 tcgaggccac gtcctgtcat tggcactaca gatgtatggc agccgtgtta tcgagaaagc
     5821 tcttgagttt attccttcag accagcagaa tgagatggtt cgggaactag atggccatgt
     5881 cttgaagtgt gtgaaagatc agaatggcaa tcacgtggtt cagaaatgca ttgaatgtgt
     5941 acagccccag tctttgcaat ttatcatcga cgcgtttaag ggacaggtat ttgccttatc
     6001 cacacatcct tatggctgcc gagtgattca gagaatcctg gagcactgtc tccctgacca
     6061 gacactccct attttagagg agcttcacca gcacacagag cagctggtac aggatcaata
     6121 tggaagttat gtaatcgaac atgtactgga gcacggtcgt cctgaggata aaagcaaaat
     6181 tgtagcagaa atccgaggca atgtacttgt attgagtcag cacaaatttg caaacaatgt
     6241 tgtgcagaag tgtgttactc acgcctcacg tacggagcgc gctgtgctca tcgacgaggt
     6301 gtgcaccatg aacgacggtc cccacagtgc cttatacacc atgatgaagg accagtatgc
     6361 caactacgtg gtccagaaga tgattgacgt ggcggagcca ggccagcgga agatcgtcat
     6421 gcataagatc cggccccaca tcgcaactct tcgtaagtac acctatggca agcacattct
     6481 ggccaagctg gagaagtact acatgaagaa cggtgttgac ttaggggacc caaagaagaa
     6541 gcgcaaagtg gatcctaaaa agaaaagaaa ggtaggcggc cgcgggggag gcggttccgg
     6601 tggcggcgga agcggaggtg gaggatcagg gccggcctga ttaattaaga attcgaccca
     6661 gctttcttgt acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct
     6721 acgtagtaat gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt
     6781 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6841 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6901 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6961 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7021 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7081 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7141 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     7201 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7261 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7321 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     7381 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7441 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7501 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7561 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7621 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     7681 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7741 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7801 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7861 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7921 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7981 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     8041 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     8101 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     8161 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     8221 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     8281 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     8341 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     8401 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     8461 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     8521 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     8581 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     8641 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     8701 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8761 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8821 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8881 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8941 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9001 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9061 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9121 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9181 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9241 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9301 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9361 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9421 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9481 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9541 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9601 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9661 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9721 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9781 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9841 gatcaaagga tcttc
//
