LOCUS       sequence-271905-        7777 bp    DNA     circular SYN 14-APR-2025
DEFINITION  N-terminal myc-tagged mouse KIF5A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Twelvetrees AE, Yuen EY, Arancibia-Carcamo IL, MacAskill AF, 
            Rostaing P, Lumb MJ, Humbert S, Triller A, Saudou F, Yan Z, Kittler 
            JT
  TITLE     Delivery of GABAARs to synapses is mediated by HAP1-KIF5 and 
            disrupted by mutant huntingtin.
  JOURNAL   Neuron. 2010 Jan 14;65(1):53-65. doi: 10.1016/j.neuron.2009.12.007.
  PUBMED    20152113
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.12"; journalName: "Neuron"; date: 
            "2010-01-14- 14"; volume: "65"; issue: "1"; pages: "53-65"
COMMENT     Sequence Label: myc-KIF5A
FEATURES             Location/Qualifiers
     source          1..7777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      912..921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             921..950
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      4064..4073
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4077..4211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4114..4133)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4168..4187
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4280..4637
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4280..4471
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4303..4323)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4488..4623
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4657..4674)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4657..4673)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4666..4688)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4886..5341
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4973..4992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5183..5204
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5358..5377)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5477..5499
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5537..5555)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5623..5727
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5728..5796,5797..6588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5946..5965)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6759..7347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7248..7267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7501..7518
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7635..7656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7671..7676,7677..7694,7695..7701)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7709..7725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7714..7736
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7733..7749
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7733..7749
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgattgaatt ggccaccatg gagcagaagc tgatctccga ggaggacctg ggatccatgg
      961 cggagactaa caacgaatgc agcatcaagg tgctttgccg atttcggccc ctgaaccagg
     1021 ccgagattct gcggggggac aagttcatcc ccattttcca aggggacgac agcgtcatta
     1081 ttgggggaaa gccatatgtc tttgaccgcg tcttcccccc aaacaccact caggagcagg
     1141 tttaccacgc ctgtgccatg cagatcgtca aagacgtcct tgctggttac aatggcacaa
     1201 tcttcgctta tggacagaca tcctcaggga aaacgcatac catggagggg aagctgcacg
     1261 accctcagct gatgggcatc attccccgga tcgctcgaga catcttcaac cacatctact
     1321 ccatggatga gaaccttgaa ttccacatta aggtatctta cttcgagatt tacctggata
     1381 agatccgtga ccttttggat gtgaccaaga cgaacctgtc cgtgcatgag gacaaaaacc
     1441 gggtgccgtt tgtcaagggt tgtaccgaac gctttgtgtc cagcccagag gagattctgg
     1501 atgtgatcga tgaggggaag tccaaccgtc acgtagctgt caccaacatg aacgagcaca
     1561 gttctcggag ccacagcatc ttcctcatca acatcaagca ggagaacgta gagaccgagc
     1621 agaagctcag cgggaagctg tacctcgtgg atctggccgg aagcgagaag gtcagcaaga
     1681 caggggcaga gggagccgtt ctggacgagg caaagaatat caacaagtcg ctgtcggccc
     1741 tggggaacgt gatctctgca ctggcagagg gcaccaaaag ctacgtgccg taccgcgaca
     1801 gcaaaatgac gaggattctc caggactctc tgggagggaa ctgcaggact accatgttca
     1861 tctgctgctc gccgtccagc tacaatgacg cagagaccaa gtccacgctc atgtttggac
     1921 agcgggcgaa gaccatcaag aacactgcct cagtgaatct ggagctgact gctgagcagt
     1981 ggaagaagaa gtatgagaag gagaaggaga agaccaaggc ccagaaggag acaattgcga
     2041 agctagaggc tgagcttagc cggtggcgca atggagagaa tgtgcctgag actgagcgcc
     2101 tggctggaga ggactcagct ctgggagctg agctctgcga ggagacccct gtgaatgaca
     2161 actcatccat tgtggtacgc atcgcacctg aggaaaggca gaaatatgag gaagagatcc
     2221 gccgtctcta caagcagctt gatgacaagg atgatgagat caaccagcag agccagctca
     2281 ttgagaagct gaagcagcag atgctggacc aggaagagct gctcgtgtcc actcggggag
     2341 acaacgagaa ggtccagcgg gagcttagcc acctgcagtc cgagaacgat gctgcgaagg
     2401 acgaggtgaa ggaagtgctg caggccctag aggagctggc ggtcaactac gaccagaagt
     2461 cccaggaggt ggaggagaag agccagcaga accagctgct ggtggacgag ctgtcccaga
     2521 aagtggccac catgctgtcc ctggagtccg agctacagcg gctccaggag gtcagtggac
     2581 accagcgaaa gcggatcgct gaggtgctga atgggctgat gagggacctg agtgagttca
     2641 gtgtcatcgt gggcaacggc gagattaagc tgcccgtgga gatcagtggg gccatcgagg
     2701 aggagttcac ggtggcccgg ctctacatca gcaagatcaa gtcggaggtg aagtccgtgg
     2761 ttaagcgatg tcggcagctg gagaacctcc aggtggagtg tcatcgcaag atggaggtga
     2821 ccggtaggga gctgtcatct tgccaactgc tcatctcaca gcatgaggcc aagatccgtt
     2881 cactcacgga gtacatgcag actgtggagt tgaagaaacg gcacctggaa gagtcctacg
     2941 actccctgag cgatgagctt gccaggctcc aggcgcacga aactgtacac gaggtagctc
     3001 tgaaagacaa ggagccagac acacaggacg cggaggaggt gaagaaggcc ctggaactac
     3061 agatggagaa tcaccgtgag gcccatcacc ggcagctggc ccgcctccgg gatgagatta
     3121 atgagaaaca gaaaaccatt gatgagctga aagacctgaa ccagaagctc cagttagagc
     3181 tggagaagct tcaggccgac tatgagaggc tgaagaatga agagaacgag aagagcgcca
     3241 agctccagga gctgacattt ctgtatgagc gacatgagca gtccaagcag gacctcaagg
     3301 ggctggagga gacagttgcc cgtgaactcc agaccctcca caaccttcgc aagctgttcg
     3361 ttcaagacgt cacgactcga gtcaagaaaa gtgcagaaat ggagcccgag gacagtgggg
     3421 ggattcattc ccaaaagcag aagatctcct ttcttgagaa caacctggaa cagcttacaa
     3481 aggttcacaa acagctggta cgtgacaatg cagatctgcg ttgtgagctt cctaaattgg
     3541 aaaaacgact tcgggctacg gctgagagag ttaaggccct ggagggtgca ctgaaggagg
     3601 ccaaggaggg cgctatgaag gacaagcgta gataccagca ggaggtggac cgcatcaaag
     3661 aagccgtgcg gtacaagagc tccggcaagc ggggccattc tgcccagatc gctaagcctg
     3721 tgaggcctgg ccactatcct gcctcctcac ccaccaaccc ctacggcacc cggagccccg
     3781 agtgtatcag ctacaccaac aacctcttcc agaactacca gaacctgcac ctgcaggctg
     3841 cgcctagctc cacttcagat atgtactttg ccagcagcgg agccacatct gttgccccct
     3901 tggcttccta ccagaaggcc aacatggaca atggaaatgc cacagatatc aacgacaaca
     3961 ggagtgacct gccgtgtggc tatgaggctg aggaccaggc caagcttttc cctctccacc
     4021 aagagacagc agccagctaa tctagaattc ctgcagaagc ttggccgcca tggcccaact
     4081 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4141 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4201 atgtctggat cgggaattaa ttcggcgcag caccatggcc tgaaataacc tctgaaagag
     4261 gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     4321 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4381 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4441 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4501 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4561 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4621 gcctaggctt ttgcaaaaag ctgttaacag cttggcactg gccgtcgttt tacaacgtcg
     4681 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     4741 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4801 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4861 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4921 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4981 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5041 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5101 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5161 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5221 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5281 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     5341 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     5401 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5461 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     5521 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5581 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5641 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5701 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5761 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5821 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5881 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5941 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6001 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6061 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6121 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6181 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6241 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6301 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6361 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6421 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6481 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6541 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6601 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6661 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6721 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6781 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6841 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6901 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6961 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7021 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7081 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7141 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7201 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7261 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7321 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7381 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7441 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7501 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7561 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7621 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7681 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7741 agctatgaca tgattacgaa ttaattcgag ctcgccc
//
