LOCUS       sequence-272116-        3045 bp    DNA     circular SYN 15-APR-2025
DEFINITION  AtzC expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB
  TITLE     Design and Optimization of a Cell-Free Atrazine Biosensor.
  JOURNAL   ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 
            10.1021/acssynbio.9b00388. Epub 2020 Mar 3.
  PUBMED    32078765
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00388"; journalName: "ACS Synth Biol"; date: 
            "2020-03-20- 20"; volume: "9"; issue: "3"; pages: "671-677"
COMMENT     Sequence Label: pJBL7036
FEATURES             Location/Qualifiers
     source          1..3045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1414..1432)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1428..1475
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1493..2308)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2216..2235
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2300..2319
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2430..3018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2919..2938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtggagc
       61 ccggaccatg tctaaagact tcgatttaat tatccgcaat gcatatctgt ccgaaaaaga
      121 ctccgtttac gatatcggca tcgtggggga ccgtatcatt aaaattgagg cgaaaatcga
      181 aggaaccgtg aaggacgaga tcgatgcgaa aggtaacctt gtttcgccgg gattcgtaga
      241 tgcgcacacg cacatggaca agagctttac ttctactggg gagcgccttc cgaaattttg
      301 gtcgcgcccg tacacgcgtg acgctgcgat tgaagatggt ttgaaatatt acaaaaatgc
      361 tacccacgaa gaaatcaagc gccatgttat tgaacacgcc catatgcagg tcttgcacgg
      421 caccctgtac acccgtacgc atgttgacgt agattctgtt gccaagacta aggctgtaga
      481 agccgtattg gaggcgaagg aggaattgaa agaccttatc gacatccaag tagtggcatt
      541 tgcacaatca ggtttctttg tggatttgga atcggagtct ctgatccgca aaagcctgga
      601 tatgggctgt gacttagttg gcggggttga ccccgctacc cgtgagaaca atgttgaggg
      661 gtcacttgac ttatgtttca aattggcaaa ggaatacgat gttgacatcg attaccacat
      721 ccatgatatc ggaactgttg gtgtatattc aattaaccgc ttagctcaga agacgatcga
      781 gaacggatat aagggtcgcg tcacaacatc gcatgcttgg tgcttcgcgg atgctccatc
      841 agaatggctg gatgaagcca tcccattgta taaggattcc ggtatgaagt ttgtaacgtg
      901 tttcagtagc acccctccaa caatgccagt cattaagttg ttagaagctg gtatcaacct
      961 gggttgcgcc agtgataata tccgcgactt ttgggttcct tttggaaatg gcgatatggt
     1021 ccaaggggcg cttattgaaa cgcaacgctt ggaactgaag acgaaccgtg atttaggatt
     1081 aatctggaaa atgatcacat cggagggggc ccgtgtgctg ggaatcgaga agaactatgg
     1141 catcgaagta gggaagaagg ctgatcttgt agttttgaat tcactgtccc cacagtgggc
     1201 cattatcgac caagctaaac gcctttgtgt catcaagaac ggacgtatta tcgtgaagga
     1261 cgaggtgatc gtagcttaac atggacgagc tgtacaagta actcggtacc aaattccaga
     1321 aaagaggcct cccgaaaggg gggccttttt tcgttttggt ccgtcgaccg gctgctaaca
     1381 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     1441 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa agccaattct gattagaaaa
     1501 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     1561 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     1621 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     1681 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     1741 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac
     1801 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     1861 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     1921 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     1981 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     2041 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     2101 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     2161 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     2221 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcttcgagc
     2281 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     2341 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
     2401 gagacacaac gtgagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2461 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2521 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2581 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2641 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2701 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2761 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2821 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2881 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2941 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3001 gggcggagcc tatggaaaaa cgccagcaac gcgatcccgc gaaat
//
