LOCUS       sequence-272837-       15112 bp    DNA     circular SYN 15-APR-2025
DEFINITION  iSTU-CRISPR/Cas9 with RcCat intron, NU structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15112)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL083
FEATURES             Location/Qualifiers
     source          1..15112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2005
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3519..3594
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4031..6415
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6428..6448
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6459..6707
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6728..6752
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8053..8682
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9111..10184
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10250..10444
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10680..10702)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10778..10918
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10833..10852
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10933..10950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11104..11692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11184..11203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11779..12573)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12998..13022
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13100..13274
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14364..15040)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14407..14427)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctnc cccccccccc
     1021 tctctacctt ctcaagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct
     1081 gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctactagcg ttcgtacacg
     1141 gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg
     1201 aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt
     1261 cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt
     1321 gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc
     1381 ggtcgttcaa gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat
     1441 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
     1501 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
     1561 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
     1621 aagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
     1681 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
     1741 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
     1801 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
     1861 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
     1921 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
     1981 ctgttgtttg gtgttacttc tgcagcctgc aggtagatcg ctcgtcgaca tggataagaa
     2041 gtactctatc ggactcgata tcggaactaa ctctgtggga tgggctgtga tcaccgatga
     2101 gtacaaggtg ccatctaaga agttcaaggt tctcggaaac accgataggc actctatcaa
     2161 gaaaaacctt atcggtgctc tcctcttcga ttctggtgaa actgctgagg ctaccagact
     2221 caagagaacc gctagaagaa ggtacaccag aagaaagaac aggatctgct acctccaaga
     2281 gatcttctct aacgagatgg ctaaagtgga tgattcattc ttccacaggc tcgaagagtc
     2341 attcctcgtg gaagaagata agaagcacga gaggcaccct atcttcggaa acatcgttga
     2401 tgaggtggca taccacgaga agtaccctac tatctaccac ctcagaaaga agctcgttga
     2461 ttctactgat aaggctgatc tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt
     2521 cagaggacac ttcctcatcg agggtgatct caaccctgat aactctgatg tggataagtt
     2581 gttcatccag ctcgtgcaga cctacaacca gcttttcgaa gagaacccta tcaacgcttc
     2641 aggtgtggat gctaaggcta tcctctctgc taggctctct aagtcaagaa ggcttgagaa
     2701 cctcattgct cagctccctg gtgagaagaa gaacggactt ttcggaaact tgatcgctct
     2761 ctctctcgga ctcaccccta acttcaagtc taacttcgat ctcgctgagg atgcaaagct
     2821 ccagctctca aaggatacct acgatgatga tctcgataac ctcctcgctc agatcggaga
     2881 tcagtacgct gatttgttcc tcgctgctaa gaacctctct gatgctatcc tcctcagtga
     2941 tatcctcaga gtgaacaccg agatcaccaa ggctccactc tcagcttcta tgatcaagag
     3001 atacgatgag caccaccagg atctcacact tctcaaggct cttgttagac agcagctccc
     3061 agagaagtac aaagagattt tcttcgatca gtctaagaac ggatacgctg gttacatcga
     3121 tggtggtgca tctcaagaag agttctacaa gttcatcaag cctatcctcg agaagatgga
     3181 tggaaccgag gaactcctcg tgaagctcaa tagagaggat cttctcagaa agcagaggac
     3241 cttcgataac ggatctatcc ctcatcagat ccacctcgga gagttgcacg ctatccttag
     3301 aaggcaagag gatttctacc cattcctcaa ggataacagg gaaaagattg agaagattct
     3361 caccttcaga atcccttact acgtgggacc tctcgctaga ggaaactcaa gattcgcttg
     3421 gatgaccaga aagtctgagg aaaccatcac cccttggaac ttcgaagagg taaatttcta
     3481 gtttttctcc ttcattttct tgggagacct tggtctcagt tttagagcta gaaatagcaa
     3541 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcgttagg
     3601 acccttttct ctttttattt ttttgagctt tgatctttct ttaaactgat ctatttttta
     3661 attgattggt tatggtgtaa atattacata gctttaactg ataatctgat tactttattt
     3721 cgtgtgtcta tgatgatgat gatagttaca ggtggtggat aagggtgcta gtgctcagtc
     3781 tttcatcgag aggatgacca acttcgataa gaaccttcca aacgagaagg tgctccctaa
     3841 gcactctttg ctctacgagt acttcaccgt gtacaacgag ttgaccaagg ttaagtacgt
     3901 gaccgaggga atgaggaagc ctgctttttt gtcaggtgag caaaagaagg ctatcgttga
     3961 tctcttgttc aagaccaaca gaaaggtgac cgtgaagcag ctcaaagagg attacttcaa
     4021 gaaaatcgag tgcttcgatt cagttgagat ttctggtgtt gaggataggt tcaacgcatc
     4081 tctcggaacc taccacgatc tcctcaagat cattaaggat aaggatttct tggataacga
     4141 ggaaaacgag gatatcttgg aggatatcgt tcttaccctc accctctttg aagatagaga
     4201 gatgattgaa gaaaggctca agacctacgc tcatctcttc gatgataagg tgatgaagca
     4261 gttgaagaga agaagataca ctggttgggg aaggctctca agaaagctca ttaacggaat
     4321 cagggataag cagtctggaa agacaatcct tgatttcctc aagtctgatg gattcgctaa
     4381 cagaaacttc atgcagctca tccacgatga ttctctcacc tttaaagagg atatccagaa
     4441 ggctcaggtt tcaggacagg gtgatagtct ccatgagcat atcgctaacc tcgctggatc
     4501 tcctgcaatc aagaagggaa tcctccagac tgtgaaggtt gtggatgagt tggtgaaggt
     4561 gatgggaagg cataagcctg agaacatcgt gatcgaaatg gctagagaga accagaccac
     4621 tcagaaggga cagaagaact ctagggaaag gatgaagagg atcgaggaag gtatcaaaga
     4681 gcttggatct cagatcctca aagagcaccc tgttgagaac actcagctcc agaatgagaa
     4741 gctctacctc tactacctcc agaacggaag ggatatgtat gtggatcaag agttggatat
     4801 caacaggctc tctgattacg atgttgatca tatcgtgcca cagtcattct tgaaggatga
     4861 ttctatcgat aacaaggtgc tcaccaggtc tgataagaac aggggtaaga gtgataacgt
     4921 gccaagtgaa gaggttgtga agaaaatgaa gaactattgg aggcagctcc tcaacgctaa
     4981 gctcatcact cagagaaagt tcgataactt gactaaggct gagaggggag gactctctga
     5041 attggataag gcaggattca tcaagaggca gcttgtggaa accaggcaga tcactaagca
     5101 cgttgcacag atcctcgatt ctaggatgaa caccaagtac gatgagaacg ataagttgat
     5161 cagggaagtg aaggttatca ccctcaagtc aaagctcgtg tctgatttca gaaaggattt
     5221 ccaattctac aaggtgaggg aaatcaacaa ctaccaccac gctcacgatg cttaccttaa
     5281 cgctgttgtt ggaaccgctc tcatcaagaa gtatcctaag ctcgagtcag agttcgtgta
     5341 cggtgattac aaggtgtacg atgtgaggaa gatgatcgct aagtctgagc aagagatcgg
     5401 aaaggctacc gctaagtatt tcttctactc taacatcatg aatttcttca agaccgagat
     5461 taccctcgct aacggtgaga tcagaaagag gccactcatc gagacaaacg gtgaaacagg
     5521 tgagatcgtg tgggataagg gaagggattt cgctaccgtt agaaaggtgc tctctatgcc
     5581 acaggtgaac atcgttaaga aaaccgaggt gcagaccggt ggattctcta aagagtctat
     5641 cctccctaag aggaactctg ataagctcat tgctaggaag aaggattggg accctaagaa
     5701 atacggtggt ttcgattctc ctaccgtggc ttactctgtt ctcgttgtgg ctaaggttga
     5761 gaagggaaag agtaagaagc tcaagtctgt taaggaactt ctcggaatca ctatcatgga
     5821 aaggtcatct ttcgagaaga acccaatcga tttcctcgag gctaagggat acaaagaggt
     5881 taagaaggat ctcatcatca agctcccaaa gtactcactc ttcgaactcg agaacggtag
     5941 aaagaggatg ctcgcttctg ctggtgagct tcaaaaggga aacgagcttg ctctcccatc
     6001 taagtacgtt aactttcttt acctcgcttc tcactacgag aagttgaagg gatctccaga
     6061 agataacgag cagaagcaac ttttcgttga gcagcacaag cactacttgg atgagatcat
     6121 cgagcagatc tctgagttct ctaaaagggt gatcctcgct gatgcaaacc tcgataaggt
     6181 gttgtctgct tacaacaagc acagagataa gcctatcagg gaacaggcag agaacatcat
     6241 ccatctcttc acccttacca acctcggtgc tcctgctgct ttcaagtact tcgatacaac
     6301 catcgatagg aagagataca cctctaccaa agaagtgctc gatgctaccc tcatccatca
     6361 gtctatcact ggactctacg agactaggat cgatctctca cagctcggtg gtgattcaag
     6421 ggctgatcct aagaagaaga ggaaggtttg actcgagata tgaagatgaa gatgaaatat
     6481 ttggtgtgtc aaataaaaag cttgtgtgct taagtttgtg tttttttctt ggcttgttgt
     6541 gttatgaatt tgtggctttt tctaatatta aatgaatgta agatcacatt ataatgaata
     6601 aacaaatgtt tctataatcc attgtgaatg ttttgttgga tctcttctgc agcatataac
     6661 tactgtatgt gctatggtat ggactatgga atatgattaa agataaggag ctcaaactat
     6721 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     6781 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     6841 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
     6901 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     6961 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     7021 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     7081 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     7141 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     7201 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     7261 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     7321 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     7381 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     7441 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     7501 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     7561 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     7621 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     7681 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     7741 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     7801 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acggctcaac
     7861 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     7921 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     7981 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     8041 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     8101 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     8161 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     8221 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     8281 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     8341 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     8401 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     8461 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     8521 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     8581 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     8641 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     8701 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     8761 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     8821 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     8881 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     8941 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     9001 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     9061 gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     9121 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     9181 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     9241 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     9301 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     9361 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     9421 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     9481 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     9541 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     9601 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     9661 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     9721 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     9781 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     9841 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     9901 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     9961 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
    10021 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
    10081 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
    10141 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
    10201 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg cctctttcct gtggatagca
    10261 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
    10321 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
    10381 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
    10441 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
    10501 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
    10561 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
    10621 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
    10681 ctctgacaca tgcagctccc ggaaacggtc acagcttgtc tgtaagcgga tgccgggagc
    10741 agacaagcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
    10801 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
    10861 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10921 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    10981 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    11041 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    11101 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    11161 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    11221 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    11281 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    11341 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    11401 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    11461 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    11521 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    11581 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    11641 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    11701 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    11761 ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    11821 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    11881 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    11941 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    12001 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    12061 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    12121 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    12181 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    12241 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    12301 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    12361 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    12421 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    12481 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    12541 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    12601 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    12661 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    12721 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc
    12781 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
    12841 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
    12901 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
    12961 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
    13021 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
    13081 acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga
    13141 aattttattg atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct
    13201 caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca
    13261 ttattatgga gaaaatagag cttgtcgatc gttaattaat cacctccaag atcccggacg
    13321 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
    13381 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
    13441 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
    13501 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
    13561 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
    13621 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
    13681 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
    13741 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
    13801 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
    13861 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
    13921 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
    13981 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
    14041 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc
    14101 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
    14161 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
    14221 acgagattct tcgccctccg agagctgcat caggtcggac acgctgtcga acttttcgat
    14281 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatcctag gagagataga
    14341 tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat
    14401 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
    14461 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
    14521 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
    14581 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
    14641 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
    14701 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
    14761 tagacgagag tgtcgtgctc caccatgttc acatcaatcc acttgctttg aagacgtggt
    14821 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
    14881 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
    14941 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
    15001 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
    15061 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tg
//
