LOCUS       sequence-27292-e         721 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: GST-GW1D12(aa895-1211)
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(378..402)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      429..438
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    442..576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(479..498)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     533..552
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(670..690)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ttgctgcggc atccagcaca tccacgtggg gctccagctc tgttggtcca caagcattaa
       61 gcaaatctgg gccaaaatct atgcaagatg gctggtgtgg tgatgatatg ccattgcctg
      121 gaaatcgccc cactggctgg gaagaggaag aggatgtgga gattggaatg tggaatagta
      181 attcatctca agagcttaac tcatctttaa attggccacc atatacaaag aaaatgtcat
      241 cgaagggtct gagtggcaaa aaaaggagaa gggaaagggg aatgatgaaa ggtggaaaca
      301 aacaagaaga agcgtggata aatccatttg ttaaacagtt ttcaaacatc agtttttcga
      361 gagactcacc agagtgaacc cagctttctt gtacaaagtg gttgatggcc gctgactgac
      421 taagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc
      481 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      541 tccaaactca tcaatgtatc ttatcatgtc tggatcggga aattaattcg gcgcagcacc
      601 atggccttga aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga
      661 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
      721 g
//
