LOCUS       sequence-27314-e         799 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: FLAG-PIWI(aa478-860)
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..104
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    117..141
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      672..681
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagaat taaccatgga ctacaaagac catgacggtg
       61 attataaaga tcatgacatc gattacaagg atgacgatga caagcttgcg gccatcacaa
      121 gtttgtacaa aaaagcaggc tctttaaagg aaccaattca gtcgactgga tcgatctcga
      181 gagacgctgg catgcccatc cagggccagc cgtgcttctg caaatacgcg cagggggcgg
      241 acagcgtgga gcccatgttc cggcacctga agaacacgta tgcgggcctg cagctggtgg
      301 tggtcatcct gcccggcaag acgcccgtgt acgccgaggt caagcgcgtg ggagacacgg
      361 tgctggggat ggccacgcag tgcgtgcaga tgaagaacgt gcagaggacc acgccacaga
      421 ccctgtccaa cctctgcctg aagatcaacg tcaagctggg aggcgtgaac aacatcctgc
      481 tgccccaggg caggccgccg gtgttccagc agcccgtcat ctttctggga gcagacgtca
      541 ctcacccccc cgccggggat gggaagaagc cctccattgc cgccgtggtg ggcagcatgg
      601 acgcccaccc caatcgctac tgcgccaccg tgcgcgtgca gcagcaccgg caggagatca
      661 tacaagacct ggccgccatg gtccgcgagc tcctcatcca gttctacaag tccacgcgct
      721 tcaagcccac ccgcatcatc ttctaccgcg acggtgtctc tgaaggccag ttccagcagg
      781 ttctccacca cgagttgct
//
