LOCUS       sequence-273366-        3540 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1496.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1496
FEATURES             Location/Qualifiers
     source          1..3540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..46
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    163..184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(199..204,205..222,223..229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    237..253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     242..264
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     261..277
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     261..277
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        295..313
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     295..312
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      932..941
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      936..945
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(975..994)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(976..994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1001..1018)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1001..1017)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1010..1032)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1158..1613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1245..1264)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1455..1476
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1691..1795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1796..1864,1865..2656)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2014..2033)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2827..3415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3316..3335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
       61 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      121 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      181 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      241 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctatttag
      301 gtgacactat agaatactca agctatgcat ccaacgcgtt gggagctctc ccatatggtc
      361 gacctgcagg cggccgcgaa ttcactagtg attccaggct gagaagcaaa aactcatctt
      421 tgtgattttc ttgagtatgt acctggtcac tgtgattgga aacagtctca taattctggc
      481 cattggtttg gatatacatc ttcatactcc tatgtatctt ttccttgcca acctatcttt
      541 tgctgatatt tcctcctctt cgacttcagt ccctaaaatg ctaatgaata ttcagaccaa
      601 tagtcaatcc atttcctatg agggctgcat tacacaaatg tacttttcta ttgtgtttgt
      661 tgtcattgac aattttcttt tgggggtaat ggcttatgat cgatatgtgg ctatctgcca
      721 ccctctgaat tatacaaaca tcatgcaccc caggttctgt cttttacttt ctttctgtcc
      781 atgggctcta agcaatattg ttgccctgac acatactctt ctggccaatc aattgatttt
      841 ttgcaaccat aacaccatcc aacacttctt ctgtgacttg gcccctctga tcaaactttc
      901 ctgctcagat gcaatgaatc gaattcccgc ggccgccatg gcggccggga gcatgcgacg
      961 tcgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     1021 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     1081 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     1141 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     1201 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     1261 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     1321 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     1381 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     1441 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     1501 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1561 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg
     1621 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg
     1681 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1741 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1801 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1861 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1921 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1981 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2041 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2101 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2161 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2221 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2281 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2341 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2401 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2461 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     2521 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2581 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2641 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2701 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2761 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2881 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2941 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3001 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3061 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3121 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3181 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3241 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3301 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3361 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3421 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3481 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
//
